pLV3-CMV-MCS-EF1a-mStayGold-Pu

pLV3-CMV-MCS-EF1a-mStayGold-Puro plasmid

PVT104589

pLV3-CMV-MCS-EF1a-mStayGold-Puro plasmid Information

Plasmid name: pLV3-CMV-MCS-EF1a-mStayGold-Puro
Alias: LC756333.1
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-EF1a-mStayGold-Puro plasmid Description

pLV3-CMV-MCS-EF1a-mStayGold-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCS-EF1a-mStayGold-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gtttaGaatc ggatccCTGC GATATCCAGC 2341 gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc 2401 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg 2461 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 2521 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 2581 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc 2641 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc 2701 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg 2761 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct 2821 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 2881 gtgaccggcg cctacgctag acgccaccat ggtgtctaca ggcgaggagc tgtttaccgg 2941 cgtggtgccc ttcaagttcc agctgaaggg caccatcaac ggcaagagct tcaccgtgga 3001 aggcgagggc gagggcaata gccacgaggg cagccacaaa ggcaagtacg tgtgcaccag 3061 cggcaaactg ccaatgtctt gggccgccct gggaactagc ttcggctatg gcatgaagta 3121 ctacaccaag taccccagcg gcctgaagaa ctggttccac gaggtgatgc ccgagggctt 3181 cacctacgac agacacatcc agtacaaggg cgacggcagc atccacgcca agcaccagca 3241 cttcatgaag aacggcacct accacaacat cgtggagttc accggccagg acttcaagga 3301 gaacagcccc gtgctgaccg gcgacatgga cgtgagcctg cccaacgagg tgcagcacat 3361 ccccatagat gacggcgtgg agtgcacagt gaccctgcag taccctctgc tgagcgacga 3421 gagcaagtgc gtggaggcct accagaacac catcatcaag cccctgcaca atcagccagc 3481 ccccgatgtg ccattccact ggatcagaaa gcagtacacc cagagcaagg acgacaccga 3541 ggagagagac cacatcatcc agagcgagac cctggaggcc cacctgggat ctcgcgaggg 3601 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta 3661 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 3721 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 3781 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 3841 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 3901 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 3961 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 4021 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 4081 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 4141 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 4201 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct 4261 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 4321 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 4381 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 4441 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 4501 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 4561 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 4621 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 4681 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 4741 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc 4801 tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac 4861 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 4921 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga 4981 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 5041 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 5101 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 5161 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 5221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 5341 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 5401 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 5461 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg 5521 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 5581 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 5641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 5761 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 5821 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 5881 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5941 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 6001 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 6061 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 6121 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 6181 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 6241 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 6301 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 6361 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 6421 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 6481 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 6541 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 6601 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 6661 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 6721 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 6781 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 6841 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 6901 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 6961 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 7021 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 7081 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 7141 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 7201 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 7261 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 7321 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 7381 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 7441 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7501 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 7561 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 7621 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 7681 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 7741 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 7801 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 7861 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 7921 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 7981 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 8041 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 8101 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.