pLV3-CMV-Lck-3×Myc-EF1a-Hyg pl

pLV3-CMV-Lck-3×Myc-EF1a-Hyg plasmid

PVT104336

pLV3-CMV-Lck-3×Myc-EF1a-Hyg plasmid Information

Plasmid name: pLV3-CMV-Lck(mouse)-3×Myc-EF1a-Hyg
Alias: NM_001162432.2;Hck-3; Lsk; Lskt; p56; p56Lck;
Gene lenght: 1560
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Lck-3×Myc-EF1a-Hyg plasmid Description

pLV3-CMV-Lck-3×Myc-EF1a-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Lck-3×Myc-EF1a-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agaGCCACCa tgggggcctc tgagctgacg atctcgggga tcatgggctg tgtctgcagc 2341 tcaaaccctg aagatgactg gatggagaac attgacgtgt gtgaaaactg ccactatccc 2401 atagtcccac tggacagcaa gatctcgctg cccatccgga atggctctga agtgcgggac 2461 ccactggtca cctatgaggg atctctccca ccagcatccc cgctgcaaga caacctggtt 2521 atcgccctgc acagttatga gccctcccat gatggagact tgggctttga gaagggtgaa 2581 cagctccgaa tcctggagca gagcggtgag tggtggaagg ctcagtccct gacgactggc 2641 caagaaggct tcattccctt caacttcgtg gcgaaagcaa acagcctgga gcctgaacct 2701 tggttcttca agaatctgag ccgtaaggac gccgagcggc agcttttggc gcccgggaac 2761 acgcatggat ccttcctgat ccgggaaagc gaaagcactg cggggtcctt ttccctgtcg 2821 gtcagagact tcgaccagaa ccagggagaa gtggtgaaac attacaagat ccgtaaccta 2881 gacaacggtg gcttctacat ctcccctcgt atcacttttc ccggattgca cgatctagtc 2941 cgccattaca ccaacgcctc tgatgggctg tgcacaaagt tgagccgtcc ttgccagacc 3001 cagaagcccc agaaaccatg gtgggaggac gaatgggaag ttcccaggga aacactgaag 3061 ttggtggagc ggctgggagc tggccagttc ggggaagtgt ggatggggta ctacaacgga 3121 cacacgaagg tggcggtgaa gagtctgaaa caagggagca tgtcccccga cgccttcctg 3181 gctgaggcta acctcatgaa gcagctgcag cacccgcggc tagtccggct ttatgcagtg 3241 gtcacccagg aacccatcta catcatcacg gaatacatgg agaacgggag cctagtagat 3301 tttctcaaga ctccctcggg catcaagttg aatgtcaaca aacttttgga catggcagcc 3361 cagattgcag agggcatggc gttcatcgaa gaacagaatt acatccatcg ggacctgcgc 3421 gccgccaaca tcctggtgtc tgacacgctg agctgcaaga ttgcagactt tggcctggcg 3481 cgcctcattg aggacaatga gtacacggcc cgggaggggg ccaaatttcc cattaagtgg 3541 acagcaccag aagccattaa ctatgggacc ttcaccatca agtcagacgt gtggtccttc 3601 gggatcttgc ttacagagat cgtcacccac ggtcgaatcc cttacccagg aatgaccaac 3661 cctgaagtca ttcagaacct ggagagaggc taccgcatgg tgagacctga caactgtccg 3721 gaagagctgt accacctcat gatgctgtgc tggaaggagc gcccagagga ccggcccacg 3781 tttgactacc ttcggagtgt tctggatgac ttcttcacag ccacagaggg ccagtaccag 3841 ccccagcctC TCGAGGAACA AAAACTAATA TCAGAGGAAG ATTTGGAACA GAAACTAATC 3901 AGTGAAGAAG ATTTAGAGCA AAAGTTAATT TCCGAGGAGG ACTTAtagga tccgcggccg 3961 caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc 4021 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg 4081 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 4141 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa 4201 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag 4261 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa 4321 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc 4381 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc 4441 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg 4501 cgcctacgtc gacatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat 4561 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc 4621 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg 4681 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga 4741 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca 4801 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc 4861 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt 4921 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga 4981 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca 5041 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca 5101 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga 5161 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag 5221 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct 5281 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca 5341 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat 5401 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt 5461 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg 5521 tccgagggca aaggaatagg tcgacaatca acctctggat tacaaaattt gtgaaagatt 5581 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 5641 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 5701 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 5761 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 5821 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 5881 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 5941 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 6001 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 6061 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 6121 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta 6181 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag 6241 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 6301 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 6361 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 6421 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 6481 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 6541 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 6601 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 6661 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 6721 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 6781 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc 6841 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 6901 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 6961 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 7021 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 7081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 7141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 7201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 7261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 7321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 7381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 7441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 7501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 7561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 7621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 7681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 7741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 7801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 7861 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 7921 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 7981 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 8041 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 8101 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 8161 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 8221 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 8281 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 8341 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 8401 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 8461 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 8521 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 8581 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 8641 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 8701 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 8761 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 8821 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 8881 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 8941 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 9001 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 9061 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 9121 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 9181 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 9241 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 9301 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 9361 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 9421 acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.