pCMV-E7(HPV18)-Neo plasmid

pCMV-E7(HPV18)-Neo plasmid

PVT104059

pCMV-E7(HPV18)-Neo plasmid Information

Plasmid name: pCMV-E7(HPV18)-Neo
Alias:
Gene lenght: 315
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human tumor Virus18
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-E7(HPV18)-Neo plasmid Description

pCMV-E7(HPV18)-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-E7(HPV18)-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccgccaccAT GCATGGACCT AAGGCAACAT TGCAAGACAT TGTATTGCAT 961 TTAGAGCCCC AAAATGAAAT TCCGGTTGAC CTTCTATGTC ACGAGCAATT AAGCGACTCA 1021 GAGGAAGAAA ACGATGAAAT AGATGGAGTT AATCATCAAC ATTTACCAGC CCGACGAGCC 1081 GAACCACAAC GTCACACAAT GTTGTGTATG TGTTGTAAGT GTGAAGCCAG AATTGAGCTA 1141 GTAGTAGAAA GCTCAGCAGA CGACCTTCGA GCATTCCAGC AGCTGTTTCT GAACACCCTG 1201 TCCTTTGTGT GTCCGTGGTG TGCATCCCAG CAGTAAgaat tctgcagata tccatcacac 1261 tggcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag 1321 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 1381 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 1441 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 1501 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 1561 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 1621 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 1681 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 1741 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg 1801 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 1861 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 1921 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 1981 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 2041 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag 2101 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 2161 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 2221 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 2281 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 2341 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 2401 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 2461 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 2521 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 2581 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 2641 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 2701 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 2761 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 2821 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 2881 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 2941 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 3001 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 3061 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 3121 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 3181 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 3241 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 3301 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 3361 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 3421 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3481 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 3541 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 3601 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 3661 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 3721 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 3781 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 3841 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 3901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 3961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 4021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 4081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 4141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg 4201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 4261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 4321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 4381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 4441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 4501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 4561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 4621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 4681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 4741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 4801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 4861 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 4921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 4981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 5041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 5101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 5161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 5221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 5281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 5341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 5401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 5461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 5521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 5581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 5641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 5701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.