pLV3-CMV-Eef1a2-3×FLAG-CopGFP-

pLV3-CMV-Eef1a2-3×FLAG-CopGFP-Puro plasmid

PVT103852

pLV3-CMV-Eef1a2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Eef1a2(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_007906.3;Eef1a; EEF1AL; EF-1-alpha-2; S1; STN;wasted; wst
Gene lenght: 1389
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Eef1a2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Eef1a2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Eef1a2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcaaggagaa gacacacatc 2341 aacattgtgg tcattggcca cgtggactca ggcaagtcca ccacgacagg ccacctcatc 2401 tacaagtgtg gtggcatcga caagcggacc atcgagaagt ttgagaagga ggcagcagag 2461 atggggaagg gctcttttaa atatgcctgg gtgctggaca agctgaaggc cgagcgggaa 2521 cgaggcatca ccatcgacat ctccctctgg aagtttgaga ccaccaagta ctacatcacc 2581 atcatcgatg ctccaggaca ccgagacttc atcaagaata tgattacagg cacatcccag 2641 gcggactgcg cagtgctgat cgtggcagcc ggtgtgggcg agtttgaggc gggcatctcc 2701 aagaacgggc aaacccggga acacgcactc ctggcctaca ctctgggtgt gaagcagctc 2761 attgtgggtg tcaacaagat ggactccacg gaaccagcct acagcgagaa gcgctatgat 2821 gagattgtta aggaggtcag cgcctacatc aagaagatcg gctacaaccc agccacggtg 2881 cccttcgtgc ccatctcggg ctggcatggt gacaacatgc tggagccttc acctaatatg 2941 ccatggttca agggctggaa agtagagcgt aaggaaggaa atgcaagcgg cgtgtccctg 3001 ctggaagccc tggacacaat cctgcccccc acccgcccca ctgacaagcc ccttcgtctg 3061 cctctgcagg atgtgtacaa gattgggggc attgggaccg tgcctgtggg ccgagtggag 3121 accggtatcc tccggcctgg tatggtggtg acctttgcgc cagtcaacat caccacagag 3181 gtgaagtctg tggaaatgca ccatgaggca cttagcgagg ccctgcctgg tgacaatgtc 3241 gggttcaatg tgaagaatgt gtccgttaag gatattcgcc ggggcaatgt ctgcggggac 3301 agcaaagctg acccgcctca ggaggctgcc cagttcacct ctcaggttat catcctgaac 3361 caccctgggc aaatcagcgc tggctactcg ccagtcatcg actgtcacac ggcccacatt 3421 gcctgcaagt ttgccgagct aaaggagaag attgaccgtc gttctggcaa gaagctggag 3481 gataacccca agtccctgaa gtctggtgat gcagccattg ttgagatggt ccctggaaaa 3541 cccatgtgtg tggagagctt ctcacagtac ccacctctcg gccgcttcgc cgtgcgcgac 3601 atgcggcaga ctgtggccgt gggcgtcatc aagaacgtgg agaagaagag cggcggcgca 3661 ggcaaggtca ccaagtccgc acagaaggct cagaaagcgg gcaagggatc cGACTACAAA 3721 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag 3781 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3841 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 3901 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3961 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 4021 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 4081 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 4141 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 4201 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 4261 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 4321 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 4381 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 4441 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 4501 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 4561 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 4621 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 4681 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 4741 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 4801 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 4861 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 4921 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 4981 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 5041 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 5101 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 5161 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 5221 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 5281 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5341 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5401 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5461 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5521 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5581 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5641 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5701 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 5761 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5821 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 5881 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 5941 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6001 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6061 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 6121 ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg gctgctcgcc 6181 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 6241 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctTcgc 6301 cttcgccctc agacgagtcg gatctccctt tggGccgcct ccccgcctgg tacctttaag 6361 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6421 ggaagggcta attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct 6481 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6541 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6601 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6661 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6721 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6841 atcttatcat gtctggctct agctaTCCCG CCCCTAACTC CGCCCAtccc gcccctaact 6901 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 6961 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 7021 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 7081 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 7141 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7201 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 9001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 9061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 9121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9181 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 9241 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9421 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9481 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9541 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9601 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9661 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.