pLV3-CMV-TRIM45-FLAG-TurboID-I

pLV3-CMV-TRIM45-FLAG-TurboID-IRES-Puro plasmid

PVT103663

pLV3-CMV-TRIM45-FLAG-TurboID-IRES-Puro plasmid Information

Plasmid name: pLV3-CMV-TRIM45(human)-FLAG-TurboID-IRES-Puro
Alias: NM_025188.4;RNF99
Gene lenght: 1740
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TRIM45-FLAG-TurboID-IRES-Puro plasmid Description

pLV3-CMV-TRIM45-FLAG-TurboID-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TRIM45-FLAG-TurboID-IRES-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCg atatcCTCGA GaaTTCgcca ccatgtcaga aaacagaaaa ccgctgctgg 2341 gctttgtaag caaactcact agtgggactg cacttgggaa ctcaggcaag actcactgcc 2401 ccctgtgctt ggggcttttc aaagccccca ggctcttgcc ttgtttgcat acagtttgca 2461 ccacgtgtct ggagcagctg gagcccttct cagtagtgga catccgaggg ggagactctg 2521 acacaagctc tgaggggtca atattccagg aactcaagcc acgaagtctg cagtcgcaga 2581 tcggcatcct ttgtcctgta tgtgatgctc aggtggacct gcccatgggt ggagtgaagg 2641 ctttaaccat agaccacctg gccgtgaatg atgtgatgct ggagagccta cgtggggaag 2701 gccagggcct ggtgtgtgac ctgtgcaacg acagggaagt agagaagagg tgtcagacct 2761 gcaaagccaa cctctgccac ttctgctgcc aggctcatag gcggcagaag aaaacgactt 2821 accacaccat ggtggaccta aaagacttga aaggctacag ccggattggg aagcccatcc 2881 tgtgtcctgt tcaccctgca gaggaactga ggctgttctg tgagttctgt gaccggcccg 2941 tgtgccagga ttgtgtggtg ggggagcatc gggaacaccc ctgtgacttc accagcaatg 3001 tcatccacaa gcatggggac tctgtgtggg agctcctcaa aggtactcag ccccacgtgg 3061 aggccctgga ggaagccctg gctcagatcc acataataaa cagtgccctc cagaagcgag 3121 tggaggcagt ggcagctgat gtccggacat tctcggaggg ctacattaag gccattgagg 3181 agcatcggga caagctgctg aagcagctgg aagacatacg ggcccagaag gaaaattccc 3241 tgcagctgca gaaggcccag ctggaacagt tactggcaga catgcggact ggagtggagt 3301 tcaccgagca cttgctgacc agcggctcag acttggagat cctcatcacc aagagggtgg 3361 tggtagaacg gctcaggaag ctgaacaaag ttcaatatag cacccgtcct ggagtaaatg 3421 ataagatacg cttctgtcct caggagaaag caggccagtg ccgtggctat gaaatttatg 3481 gtacgattaa taccaaagag gttgatccag ccaaatgtgt cctacaagga gaagacctcc 3541 acagagcccg ggagaaacag acggcctctt tcaccctgct ttgtaaggat gccgcaggag 3601 aaatcatggg caggggagga gacaacgttc aagttgccgt tgtccctaaa gataagaaag 3661 acagcccagt cagaacaatg gtccaggata acaaggatgg gacatactac atttcctaca 3721 cccccaagga acctggcgtc tatactgtgt gggtctgcat caaagaacag catgtgcagg 3781 gctcgccatt cactgtgatg gtgaggagaa agcaccgccc acactcaggc gtgtttcact 3841 gctgcacctt ctgctccagc gggggccaga aaaccgctcg ctgcgcctgt ggaggcacca 3901 tgccaggtgg gtacctaggc tgtggccatg gacacaaagg ccacccaggt catccccact 3961 ggtcatgctg tggaaaattt aatgagaaat ctgaatgcac atggacaggt gggcagagcg 4021 caccgaggag tctacttagg actgtggctc tcGATTACAA GGATGACGAC GATAAGGGTG 4081 GATCAAAAGA CAATACTGTG CCTCTGAAGC TGATCGCTCT CCTGGCTAAT GGCGAGTTCC 4141 ATAGTGGCGA ACAGCTGGGA GAAACCCTGG GCATGTCCAG GGCCGCTATC AACAAGCACA 4201 TTCAGACTCT GCGCGACTGG GGCGTGGACG TGTTCACCGT GCCCGGAAAG GGCTACTCTC 4261 TGCCCGAGCC TATCCCGCTG CTGAACGCTA AACAGATTCT GGGACAGCTG GACGGCGGGA 4321 GCGTGGCAGT CCTGCCTGTG GTCGACTCCA CCAATCAGTA CCTGCTGGAT CGAATCGGCG 4381 AGCTGAAGAG TGGGGATGCT TGCATTGCAG AATATCAGCA GGCAGGGAGA GGAAGCAGAG 4441 GGAGGAAATG GTTCTCTCCT TTTGGAGCTA ACCTGTACCT GAGTATGTTT TGGCGCCTGA 4501 AGCGGGGACC AGCAGCAATC GGCCTGGGCC CGGTCATCGG AATTGTCATG GCAGAAGCGC 4561 TGCGAAAGCT GGGAGCAGAC AAGGTGCGAG TCAAATGGCC CAATGACCTG TATCTGCAGG 4621 ATAGAAAGCT GGCAGGCATC CTGGTGGAGC TGGCCGGAAT AACAGGCGAT GCTGCACAGA 4681 TCGTCATTGG CGCCGGGATT AACGTGGCTA TGAGGCGCGT GGAGGAAAGC GTGGTCAATC 4741 AGGGCTGGAT CACACTGCAG GAAGCAGGGA TTAACCTGGA CAGGAATACT CTGGCCGCTA 4801 CGCTGATCCG AGAGCTGCGG GCAGCCCTGG AACTGTTCGA GCAGGAAGGC CTGGCTCCAT 4861 ATCTGCCACG GTGGGAGAAG CTGGATAACT TCATCAATAG ACCCGTGAAG CTGATCATTG 4921 GGGACAAAGA GATTTTCGGG ATTAGCCGGG GGATTGATAA ACAGGGAGCC CTGCTGCTGG 4981 AACAGGACGG AGTTATCAAA CCCTGGATGG GCGGAGAAAT CAGTCTGCGG TCTGCCGAAA 5041 AGtaGGGATC CGCGGCCGCg cccctctccc tccccccccc ctaacgttac tggccgaagc 5101 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct 5161 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt 5221 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct 5281 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc 5341 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag 5401 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc 5461 acctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga 5521 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc 5581 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggtg 5641 agcaagggcg aggaggataa catggCCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC 5701 ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC 5761 GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA 5821 CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC 5881 GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC 5941 GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC 6001 CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG 6061 CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC 6121 GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC 6181 GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG 6241 TGCATGACCC GCAAGCCCGG TGCCTGAGTC GACAATCAAC CTCTGGATTA CAAAATTTGT 6301 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 6361 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 6421 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 6481 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 6541 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 6601 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 6661 TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 6721 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 6781 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 6841 TCCCTTTGGG CCGCCTCCCC GCCTGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT 6901 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG 6961 AAAATAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 7021 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 7081 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 7141 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA 7201 TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA 7261 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 7321 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT 7381 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 7441 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 7501 GGCTTTTTTG GAGGCCTAGA CTTTTGCAGA GACGGCCCAA ATTCGTAATC ATGGTCATAG 7561 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 7621 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 7681 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 7741 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 7801 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 7861 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 7921 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 7981 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 8041 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 8101 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 8161 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 8221 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 8281 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 8341 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA 8401 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 8461 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 8521 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 8581 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 8641 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 8701 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 8761 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 8821 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT 8881 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 8941 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 9001 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 9061 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 9121 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 9181 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 9241 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 9301 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 9361 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 9421 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 9481 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 9541 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 9601 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 9661 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC 9721 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG 9781 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT 9841 TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC 9901 GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT 9961 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG 10021 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC 10081 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC 10141 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.