pLV3-CMV-AARS2-FLAG-TurboID-Pu

pLV3-CMV-AARS2-FLAG-TurboID-Puro plasmid

PVT103739

pLV3-CMV-AARS2-FLAG-TurboID-Puro plasmid Information

Plasmid name: pLV3-CMV-AARS2(human)-FLAG-TurboID-Puro
Alias: NM_020745.4;AARSL; COXPD8; LKENP; MT-ALARS; MTALARS
Gene lenght: 2955
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-AARS2-FLAG-TurboID-Puro plasmid Description

pLV3-CMV-AARS2-FLAG-TurboID-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-AARS2-FLAG-TurboID-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggcagcg tcagtggcag ctgcagcccg gaggctgcgg 2341 cgggccattc gaaggtcgcc cgcatggcgg ggcctcagcc atcggccgct ctcatcggag 2401 ccccctgcag ccaaggcctc ggccgtgagg gccgcctttc tgaacttctt tcgggaccgc 2461 catggccacc ggctggtgcc ctccgcttcc gtgcggcccc gcggcgaccc cagtttgctt 2521 tttgtcaatg cgggcatgaa ccagttcaag ccaatctttc tgggcaccgt ggatccacga 2581 agcgagatgg caggcttccg acgtgtggcc aacagccaga aatgtgtgag agctggagga 2641 caccataacg acctggaaga tgtgggtcga gacctttccc atcatacctt ctttgaaatg 2701 cttggcaatt gggcctttgg gggtgaatat tttaaggagg aggcttgtaa catggcctgg 2761 gaactgctga ctcaggtcta tgggatccct gaggaaaggc tctggatctc ctactttgat 2821 ggtgacccca aggcagggct ggacccagac ctggagacca gggacatctg gctgagctta 2881 ggggtgcctg ctagccgtgt gctttccttt ggaccacaag agaacttctg ggagatgggg 2941 gatactggcc cttgtgggcc ctgtactgag atccactacg accttgctgg tggggtggga 3001 gccccccagc tggtagagct ttggaacctg gtcttcatgc aacacaacag agaggcagat 3061 ggaagcctgc agcccctgcc ccagcggcat gtggacacag gaatgggcct ggaaaggctg 3121 gtggctgtgc tgcaaggcaa acactccacc tatgacactg acctcttttc cccgctgctc 3181 aacgccatac agcagggctg cagggcaccc ccttacttgg gccgagtagg ggtggcagac 3241 gaggggcgca cagacacagc gtaccgcgtg gtggctgacc acatccgcac actcagtgtc 3301 tgcatctctg atggcatctt ccctgggatg tcaggtcccc cgctggttct tcgtcggatc 3361 ctgcgtcgag ctgtgcgttt ctccatggag atcttaaagg caccacctgg cttcctaggc 3421 agcctggtac ctgtagtggt ggagacactg ggagatgctt atccagaact gcaaaggaac 3481 tcagcccaga tcgccaacct ggtgtcagag gacgaggcag ccttcctggc ctccctggag 3541 cggggtaggc ggatcattga tcggactctg aggaccctgg ggccttcaga tatgttccct 3601 gctgaagtgg cctggtcctt gtcactgtgt ggagacctgg gactcccctt ggacatggta 3661 gagctgatgc tggaggagaa aggggtccag ctagactccg ctggactgga gcggttggcc 3721 caagaggagg cccagcaccg ggcacggcag gctgagccag ttcagaagca gggattgtgg 3781 cttgatgtcc atgcgcttgg ggagctgcag cgccaaggag tgcccccaac tgacgacagc 3841 cccaagtaca actactccct gcgacccagc ggaagttatg agttcggcac ctgtgaggcc 3901 caggtgttgc aactgtatac agaggacggg acagcagtgg cctccgtggg gaaaggccag 3961 cgctgtggcc tcctcttgga caggaccaac ttctacgcag aacagggggg ccaggcttca 4021 gaccgtggct acctggtgcg ggcagggcaa gaggacgtgc tgttcccagt agcccgggcc 4081 caggtctgtg gaggtttcat cctgcatgag gcagtagccc ctgagtgcct gcggttaggg 4141 gaccaggtgc agctgcatgt ggatgaggcc tggcgtctag gctgcatggc gaagcatacg 4201 gccacccacc tgctgaactg ggcactgagg cagaccctgg gccctggcac agagcagcag 4261 ggctcccatc tcaatcctga gcagctgcgc ttggatgtga ccacccagac cccattgacc 4321 ccagagcagc tccgggcagt ggagaacact gtgcaggagg ccgtggggca ggatgaggct 4381 gtgtacatgg aggaggtgcc cctggcgctc actgcccagg tccctggcct gcgctctctg 4441 gatgaggttt acccagaccc tgtgcgggtg gtatcagtgg gggtgcccgt ggcccatgca 4501 ttggacccag cctcccaagc cgcactgcag acctctgtgg agctatgctg tgggacgcac 4561 ctgttacgta ctggggctgt aggggacctg gttatcatcg gggaccgcca gctttccaag 4621 ggcactaccc gcctgctggc cgtcactggg gagcaggccc agcaggcccg agagctaggc 4681 cagagcctgg cccaggaagt gaaagcggcc actgagcggc tgagtctggg gagccgggat 4741 gtggcggagg cactgaggct gtccaaggac ataggacgac tcattgaagc tgtggaaact 4801 gctgtgatgc cccagtggca gcggcgggag ctgctggcca cagtgaagat gctgcagcgg 4861 cgtgccaaca ctgccatccg taagctgcaa atgggacagg ctgcaaagaa aactcaggag 4921 ctgctggagc ggcactcgaa ggggcctctg attgtggaca cagtctctgc tgagtctctc 4981 tcagtgctgg tgaaggtggt acggcagctg tgtgagcagg cccccagcac gtctgtgctc 5041 ctactcagcc cccagcccat ggggaaggtg ctgtgtgcct gtcaggtggc ccagggtgcc 5101 atgcccacct tcacagcaga ggcctgggca ctggcagtgt gcagccacat ggggggcaag 5161 gcgtggggct cacgagtggt ggcccaaggc accggaagca ctactgacct ggaagctgcc 5221 ctcagtatag cccaaaccta tgccctcagc cagctcGGAT CCGATTACAA GGATGACGAC 5281 GATAAGGGTG GATCAAAAGA CAATACTGTG CCTCTGAAGC TGATCGCTCT CCTGGCTAAT 5341 GGCGAGTTCC ATAGTGGCGA ACAGCTGGGA GAAACCCTGG GCATGTCCAG GGCCGCTATC 5401 AACAAGCACA TTCAGACTCT GCGCGACTGG GGCGTGGACG TGTTCACCGT GCCCGGAAAG 5461 GGCTACTCTC TGCCCGAGCC TATCCCGCTG CTGAACGCTA AACAGATTCT GGGACAGCTG 5521 GACGGCGGGA GCGTGGCAGT CCTGCCTGTG GTCGACTCCA CCAATCAGTA CCTGCTGGAT 5581 CGAATCGGCG AGCTGAAGAG TGGGGATGCT TGCATTGCAG AATATCAGCA GGCAGGGAGA 5641 GGAAGCAGAG GGAGGAAATG GTTCTCTCCT TTTGGAGCTA ACCTGTACCT GAGTATGTTT 5701 TGGCGCCTGA AGCGGGGACC AGCAGCAATC GGCCTGGGCC CGGTCATCGG AATTGTCATG 5761 GCAGAAGCGC TGCGAAAGCT GGGAGCAGAC AAGGTGCGAG TCAAATGGCC CAATGACCTG 5821 TATCTGCAGG ATAGAAAGCT GGCAGGCATC CTGGTGGAGC TGGCCGGAAT AACAGGCGAT 5881 GCTGCACAGA TCGTCATTGG CGCCGGGATT AACGTGGCTA TGAGGCGCGT GGAGGAAAGC 5941 GTGGTCAATC AGGGCTGGAT CACACTGCAG GAAGCAGGGA TTAACCTGGA CAGGAATACT 6001 CTGGCCGCTA CGCTGATCCG AGAGCTGCGG GCAGCCCTGG AACTGTTCGA GCAGGAAGGC 6061 CTGGCTCCAT ATCTGCCACG GTGGGAGAAG CTGGATAACT TCATCAATAG ACCCGTGAAG 6121 CTGATCATTG GGGACAAAGA GATTTTCGGG ATTAGCCGGG GGATTGATAA ACAGGGAGCC 6181 CTGCTGCTGG AACAGGACGG AGTTATCAAA CCCTGGATGG GCGGAGAAAT CAGTCTGCGG 6241 TCTGCCGAAA AGtaGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA 6301 GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT 6361 GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT 6421 TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT 6481 CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA 6541 CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT 6601 CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG 6661 AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG 6721 CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT 6781 TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGATGACC GAGTACAAGC CCACGGTGCG 6841 CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 6901 CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT 6961 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 7021 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 7081 CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 7141 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 7201 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 7261 GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 7321 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 7381 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGTCGAC AATCAACCTC TGGATTACAA 7441 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 7501 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 7561 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 7621 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 7681 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 7741 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 7801 GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT 7861 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 7921 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG 7981 TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGTACCTTT AAGACCAATG ACTTACAAGG 8041 CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT 8101 CCCAACGAAA ATAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT 8161 GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC 8221 CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC 8281 TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA 8341 TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG 8401 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 8461 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC 8521 TCTAGCTATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 8581 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA 8641 GTGAGGAGGC TTTTTTGGAG GCCTAGACTT TTGCAGAGAC GGCCCAAATT CGTAATCATG 8701 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 8761 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 8821 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 8881 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 8941 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 9001 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 9061 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 9121 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 9181 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 9241 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 9301 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 9361 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 9421 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 9481 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 9541 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 9601 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 9661 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 9721 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 9781 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 9841 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 9901 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 9961 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC 10021 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 10081 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 10141 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 10201 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 10261 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 10321 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 10381 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 10441 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 10501 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 10561 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 10621 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 10681 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 10741 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 10801 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA 10861 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG 10921 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 10981 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 11041 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 11101 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 11161 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 11221 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 11281 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.