pCMV-Extl1-3×FLAG-Neo plasmid

pCMV-Extl1-3×FLAG-Neo plasmid

PVT103182

pCMV-Extl1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Extl1(mouse)-3×FLAG-Neo
Alias: NM_019578.2;D430033M16Rik;
Gene lenght: 2007
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Extl1-3×FLAG-Neo plasmid Description

pCMV-Extl1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Extl1-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCgccacc ATGCTGTGGA GAAGAAAGTC 961 CTTCTGGCTG GCGTTGTCAG CCTTCTGGCT CCTCCTCGTC CTCCTAGGAG TCTTCCCCCT 1021 TCGCCTGGCA GTGTTGCCCG GTCCTCTTCC AGGGCGCTCC CAAGGCTGGC CCCGCTGGCT 1081 GGATGCTGCC TTCTTACAGA GTTTCTCCCA GTCTGAGACG AACCCAGAAG ATGTCGCACA 1141 GCTCCCTCGG GTCTCTCGGG GAAGCAGCTG CACCTGGGGA GCCTGCTTTG ACACCTCCAA 1201 GTGTAGGGGA AAGGTCCTCA AGATCTTTGT GCACTCCCCG GCTGGTCCGA CCTCTGAGGC 1261 TCAGCGCAGG ATCCTGGATT CCTTGGAAGG CTCCCGCTAC TCTGCACTCA GCCCAGCAGA 1321 CGCTTGCCTT CTCCTTTTTC TCCCCAGCCA GGATCGTAGA GGAGCCTGCG GGCCCCTGCC 1381 TCCGAACTGG AACGGAGGCC GGAACCATCT GGTGTTGAGT CTCTACCCAG CCCCTTGCAC 1441 CAGGCTGGGA CAGGCGATGG TAGCTGAGGC CAGCCCCTCC TCGGACATCT TCCGACCGGG 1501 CTTCGATTTA GCGCTCCCGT ATCTCCCCGA AGCTCACCCA TTGCGAGGTG GGGCTCCCGG 1561 CAAGCTGCAG CAGCACAGTC CCCAACCTGG GGCCACCTTA CTGGCGGTAG CAGAGGAGAA 1621 AGGCAGGTGG CGTATCACAA GCACCCACGC CTCAGCCTGC CTCTGGGACA GGCACTGTGA 1681 GCAAGACCCT GGACCCCAGC AGACCTACCC AGGAGAGACC CTTCCCAATG CCACCTTCTG 1741 CCTCATCCCT GGTCACCGAT CTGCCACCTC CTGCTTTCTC CAAGCCCTTC AGGCCGGCTG 1801 CATCCCCGTG CTCCTCAGCC CCCGCTGGGA GCTGCCTTTC TCTGAAGTCA TCGACTGGAC 1861 CAAGGCAGCC ATCATTGCTG ATGAGAGACT CCCACTGCAG GTCCTGGCTG CCCTCCGTGA 1921 AATGCTCCCT TCGAGGGTCC TCGCCCTGCG CCAGCAGACC CAGTTTCTGT GGACCGCTTA 1981 CTTCTCCTCG GTGGAAAAAG TTATCCATAC CACCCTAGAG ATTATTCAGG ACCGGATCTG 2041 GGGTGCATCA GGTCACCCCT CACTGATGTG GAATAGCCCC CCGGGAGCAC TCTTGGCGCT 2101 GCCCACATTT TCCACGAGCC TTCAAGACTT CCCCTTTTAT CATCTGCAAC TGGGCTCTGG 2161 CCCTGGGAGC AGTTTCAGCG CCGTGATCTG GGTGGGAGCC TCTGGAGAGT CCCTGCTGAA 2221 GCTCATCCAG GAGGTGGCAG GTTCTCGGCA CTGTGCCCAG ATCCTGATTC TCTGGAACAG 2281 TGAGAAGCTG CCCCCTGACA GGTGGCCCGA GACAGCAGTG CCCCTAACAG TCATCAAGGG 2341 GCACAGGAAG GTCAGCAACC GGTTCTTCCC GTACAGCAAC ATCAGCACCA ATGTTATCCT 2401 CAGCCTTGAT GCTCAGAGCA CTCTTTCTAC CAGTGAGGTG GACTTTGCCT TTGTGGTGTG 2461 GCAGAGCTTT CCTGAGCGGA TGGTGGGCTT TCTGTCTGGA AGCCACTTCT GGGATGAGGC 2521 CCAGGGTGGC TGGGGCTACC GTACTGGGAT GACCAATGAG TTCTCCATGG TCCTGACCAC 2581 GGCTGCCTTC TACCACAGGT ACTACCACAC CCTCTTCACC CACTCCCTGC CTAAGGCCCT 2641 GAGGACCATA GCTGATGAGA CTCCCACCTG TGTTGACGTC CTGATGAACT TCCTGGTAGC 2701 CACCGTCACC AAACTGCCCC CTATCAAGGT GCCTTATGGG AGGCAGCACC CGGAGGCTGT 2761 GCCAATGGAT TCTGGGGATC CACGACCAGT GCCGGAGCCT CAGCCCCTGG ATCAGGACTG 2821 CATCAACCGT TTAGCAGCAG GGTTCGGCCA CATGCCTCTG GTTTCCTCTC AAGTGCGTCT 2881 GGATCCGGTG TTGTTCAAGG ACCCGGTATC GGTGCAACGC AAGAAGTATC GCAGTCTAGA 2941 GAAGCCAgGA TATCCAGCAC AGTGGCGGCC GCTCGAGGAC TACAAAGACC ATGACGGTGA 3001 TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG GGCCCGTTTA 3061 AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 3121 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 3181 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 3241 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC 3301 TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG 3361 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 3421 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 3481 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 3541 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 3601 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 3661 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 3721 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA 3781 ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 3841 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 3901 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 3961 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 4021 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 4081 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 4141 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 4201 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 4261 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 4321 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 4381 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 4441 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 4501 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 4561 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 4621 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 4681 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 4741 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 4801 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 4861 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 4921 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 4981 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 5041 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 5101 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 5161 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 5221 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 5281 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 5341 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 5401 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 5461 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 5521 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 5581 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 5641 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 5701 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 5761 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 5821 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 5881 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 5941 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 6001 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 6061 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 6121 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 6181 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 6241 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 6301 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 6361 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 6421 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 6481 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 6541 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 6601 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 6661 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 6721 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 6781 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 6841 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 6901 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 6961 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 7021 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 7081 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 7141 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 7201 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 7261 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 7321 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 7381 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 7441 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT C

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.