pLV3-CMV-VAV3-3×FLAG-CopGFP-Pu

pLV3-CMV-VAV3-3×FLAG-CopGFP-Puro plasmid

PVT102787

pLV3-CMV-VAV3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-VAV3(human)-3×FLAG-CopGFP-Puro
Alias: NM_006113.5;
Gene lenght: 2541
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-VAV3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-VAV3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-VAV3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agccgtggaa gcagtgcgcg 2341 cagtggctca tccattgcaa ggtgctgccc accaaccacc gggtgacctg ggactcggct 2401 caggtgttcg accttgcgca gaccctccgc gatggagtcc tgctctgcca gctgcttaac 2461 aacctccggg cgcactccat caacctgaag gagatcaacc tgaggccgca gatgtcccag 2521 tttctctgtt tgaagaacat aaggacattt ctcacggcct gttgtgagac gtttggaatg 2581 aggaaaagtg aacttttcga ggcatttgac ttgtttgatg ttcgtgactt tggaaaggtt 2641 atagaaacat tatcacgact ttctcgaaca cctatagcat tggccacagg aatcaggccc 2701 ttcccaacag aagaaagcat taatgatgaa gacatctaca aaggccttcc tgatttaata 2761 gatgaaaccc ttgtggaaga tgaagaagat ctctatgact gtgtttatgg ggaagatgaa 2821 ggtggagaag tctatgagga cttaatgaag gcagaggaag cacatcagcc caaatgtcca 2881 gaaaatgata tacgaagttg ttgtctagca gaaattaagc agacagaaga aaaatataca 2941 gaaactttgg agtcaataga aaagtatttc atggcaccac taaaaagatt tctgacagca 3001 gcagaatttg attcagtatt catcaacatt cctgaacttg taaaacttca tcggaaccta 3061 atgcaagaga ttcatgattc cattgtaaat aaaaatgacc agaacttgta ccaagttttt 3121 attaactaca aggaaagatt ggttatttac gggcagtact gcagtggagt ggagtcagcc 3181 atctctagtt tagactacat ttctaagaca aaagaagatg tcaaactgaa attagaggaa 3241 tgttccaaaa gagcaaataa tgggaaattt actcttcgag acttgcttgt ggttcctatg 3301 caacgtgttt taaagtacca ccttctcctc caggaactgg tcaaacatac cactgatccg 3361 actgagaagg caaatctgaa actggctctt gatgccatga aggacttggc acaatatgtg 3421 aatgaagtga aaagagataa tgagaccctt cgtgaaatta aacagtttca gctatctata 3481 gagaatttga accaaccagt tttgcttttt ggacgacctc agggagatgg tgaaattcga 3541 ataaccactc tagacaagca taccaaacaa gaaaggcata tcttcttatt tgatttggca 3601 gtgatcgtat gtaagagaaa aggtgataac tatgaaatga aggaaataat agatcttcag 3661 cagtacaaga tagccaataa tcctacaacc gataaagaaa acaaaaagtg gtcttatggc 3721 ttctacctca tccataccca aggacaaaat gggttagaat tttattgcaa aacaaaagat 3781 ttaaagaaga aatggctaga acagtttgaa atggctttgt ctaacataag accagactat 3841 gcagactcca atttccacga cttcaagatg cataccttca ctcgagtcac atcctgcaaa 3901 gtctgccaga tgctcctgag gggaacattt tatcaaggct atttatgttt taagtgtgga 3961 gcgagagcac acaaagaatg tttgggaaga gtagacaatt gtggcagagt taattctggt 4021 gaacaaggga cactcaaact accagagaaa cggaccaatg gactgcgaag aactcctaaa 4081 caggtggatc caggtttacc aaagatgcag gtcattagga actattctgg aacaccaccc 4141 ccagctctgc atgaaggacc ccctttacag ctccaggccg gggataccgt tgaacttctg 4201 aaaggagatg cacacagtct gttttggcag ggcagaaatt tagcatctgg agaggttgga 4261 ttttttccaa gtgatgcagt caagccttgc ccatgtgtgc ccaaaccagt agattattct 4321 tgccaaccct ggtatgctgg agcaatggaa agattgcaag cagagaccga acttattaat 4381 agggtaaata gtacttacct tgtgaggcac aggaccaaag agtcaggaga atatgcaatt 4441 agcattaagt acaataatga agcaaagcac atcaagattt taacaagaga tggctttttt 4501 cacattgcag aaaatagaaa atttaaaagt ttaatggaac ttgtggagta ctacaagcat 4561 cattctctca aggaagggtt cagaacctta gatacaactc tgcagtttcc atacaaggag 4621 ccagaacatt cagctggaca gaggggtaat agagcaggca acagcttgtt aagtccaaaa 4681 gtgctgggca ttgccatcgc tcggtatgac ttctgtgcaa gagatatgag agagttgtcc 4741 ttgttgaaag gagatgtggt gaagatttac acaaagatga gtgcaaatgg ctggtggaga 4801 ggagaagtaa atggcagggt gggctggttt ccatccacat atgtggaaga ggatgaagga 4861 tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC 4921 GATGACAAGt agcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 4981 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 5041 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 5101 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 5161 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 5221 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 5281 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 5341 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 5401 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 5461 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 5521 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 5581 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 5641 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 5701 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 5761 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 5821 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 5881 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 5941 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 6001 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 6061 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 6121 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 6181 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 6241 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 6301 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 6361 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 6421 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 6481 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 6541 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 6601 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 6661 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 6721 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 6781 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 6841 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 6901 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 6961 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 7021 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 7081 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 7141 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 7201 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 7261 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaATCATC GTCCTTTCCT 7321 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 7381 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 7441 ccgcgtctTc gccttcgccc tcagacgagt cggatctccc tttggGccgc ctccccgcct 7501 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 7561 aaagggggga ctggaagggc taattcactc ccaacgaaGa taagatctgc tttttgcttg 7621 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7681 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 7741 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7801 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7861 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 7921 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 7981 actcatcaat gtatcttatc atgtctggct ctagctaTCC CGCCCCTAAC TCCGCCCAtc 8041 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 8101 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 8161 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc 8221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 8341 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 8401 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 8461 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 8521 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 9061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 9121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 9181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 9361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 9421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 9481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 10021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 10081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 10141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 10201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 10261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 10321 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 10381 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 10441 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 10501 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 10561 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 10621 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 10681 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 10741 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 10801 acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.