pT7-6×His-KDM1A-6×His plasmid

pT7-6×His-KDM1A-6×His plasmid

PVT102672

pT7-6×His-KDM1A-6×His plasmid Information

Plasmid name: pT7-6×His-KDM1A(human)-6×His
Alias: NM_001009999.3;AIMAH3; AOF2; BHC110; CPRF; KDM1; LSD1
Gene lenght: 2628
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-6×His-KDM1A-6×His plasmid Description

pT7-6×His-KDM1A-6×His is a plasmid suitable for protein expression in Escherichia coli.

pT7-6×His-KDM1A-6×His plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa 5161 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatgg ttctggttct 5221 ggccatatgc accatcatca tcatcattct tctggtctgg tgccacgcgg ttctggtatg 5281 aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg acagcccaga tctgggtacc 5341 gacgacgacg acaaggccat ggctgatatc ggatccatgt tatctgggaa gaaggcggca 5401 gccgcggcgg cggcggctgc agcggcagca accgggacgg aggctggccc tgggacagca 5461 ggcggctccg agaacgggtc tgaggtggcc gcgcagcccg cgggcctgtc gggcccagcc 5521 gaggtcgggc cgggggcggt gggggagcgc acaccccgca agaaagagcc tccgcgggcc 5581 tcgccccccg ggggcctggc ggaaccgccg gggtccgcag ggcctcaggc cggccctact 5641 gtcgtgcctg ggtctgcgac ccccatggaa actggaatag cagagactcc ggaggggcgt 5701 cggaccagcc ggcgcaagcg ggcgaaggta gagtacagag agatggatga aagcttggcc 5761 aacctctcag aagatgagta ttattcagaa gaagagagaa atgccaaagc agagaaggaa 5821 aagaagcttc ccccaccacc ccctcaagcc ccacctgagg aagaaaatga aagtgagcct 5881 gaagaaccat cggggcaagc aggaggactt caagacgaca gttctggagg gtatggagac 5941 ggccaagcat caggtgtgga gggcgcagct ttccagagcc gacttcctca tgaccggatg 6001 acttctcaag aagcagcctg ttttccagat attatcagtg gaccacaaca gacccagaag 6061 gtttttcttt tcattagaaa ccgcacactg cagttgtggt tggataatcc aaagattcag 6121 ctgacatttg aggctactct ccaacaatta gaagcacctt ataacagtga tactgtgctt 6181 gtccaccgag ttcacagtta tttagagcgt catggtctta tcaacttcgg catctataag 6241 aggataaaac ccctaccaac taaaaagaca ggaaaggtaa ttattatagg ctctggggtc 6301 tcaggcttgg cagcagctcg acagttacaa agttttggaa tggatgtcac acttttggaa 6361 gccagggatc gtgtgggtgg acgagttgcc acatttcgca aaggaaacta tgtagctgat 6421 cttggagcca tggtggtaac aggtcttgga gggaatccta tggctgtggt cagcaaacaa 6481 gtaaatatgg aactggccaa gatcaagcaa aaatgcccac tttatgaagc caacggacaa 6541 gctgacactg tcaaggttcc taaagagaaa gatgaaatgg tagagcaaga gtttaaccgg 6601 ttgctagaag ctacatctta ccttagtcat caactagact tcaatgtcct caataataag 6661 cctgtgtccc ttggccaggc attggaagtt gtcattcagt tacaagagaa gcatgtcaaa 6721 gatgagcaga ttgaacattg gaagaagata gtgaaaactc aggaagaatt gaaagaactt 6781 cttaataaga tggtaaattt gaaagagaaa attaaagaac tccatcagca atacaaagaa 6841 gcatctgaag taaagccacc cagagatatt actgccgagt tcttagtgaa aagcaaacac 6901 agggatctga ccgccctatg caaggaatat gatgaattag ctgaaacaca aggaaagcta 6961 gaagaaaaac ttcaggagtt ggaagcgaat cccccaagtg atgtatatct ctcatcaaga 7021 gacagacaaa tacttgattg gcattttgca aatcttgaat ttgctaatgc cacacctctc 7081 tcaactctct cccttaagca ctgggatcag gatgatgact ttgagttcac tggcagccac 7141 ctgacagtaa ggaatggcta ctcgtgtgtg cctgtggctt tagcagaagg cctagacatt 7201 aaactgaata cagcagtgcg acaggttcgc tacacggctt caggatgtga agtgatagct 7261 gtgaataccc gctccacgag tcaaaccttt atttataaat gcgacgcagt tctctgtacc 7321 cttcccctgg gtgtgctgaa gcagcagcca ccagccgttc agtttgtgcc acctctccct 7381 gagtggaaaa catctgcagt ccaaaggatg ggatttggca accttaacaa ggtggtgttg 7441 tgttttgatc gggtgttctg ggatccaagt gtcaatttgt tcgggcatgt tggcagtacg 7501 actgccagca ggggtgagct cttcctcttc tggaacctct ataaagctcc aatactgttg 7561 gcactagtgg caggagaagc tgctggtatc atggaaaaca taagtgacga tgtgattgtt 7621 ggccgatgcc tggccattct caaagggatt tttggtagca gtgcagtacc tcagcccaaa 7681 gaaactgtgg tgtctcgttg gcgtgctgat ccctgggctc ggggctctta ttcctatgtt 7741 gctgcaggat catctggaaa tgactatgat ttaatggctc agccaatcac tcctggcccc 7801 tcgattccag gtgccccaca gccgattcca cgactcttct ttgcgggaga acatacgatc 7861 cgtaactacc cagccacagt gcatggtgct ctgctgagtg ggctgcgaga agcgggaaga 7921 attgcagacc agtttttggg ggccatgtat acgctgcctc gccaggccac accaggtgtt 7981 cctgcacagc agtccccaag catgctcgag caccaccacc accaccactg agatccggct 8041 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca 8101 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata 8161 tccggat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.