pLV3-CMV-Rad51d-3×FLAG-CopGFP-

pLV3-CMV-Rad51d-3×FLAG-CopGFP-Puro plasmid

PVT101958

pLV3-CMV-Rad51d-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Rad51d(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_011235.4;R51H3; Rad51l3; TRAD; Trad-d5
Gene lenght: 987
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Rad51d-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Rad51d-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Rad51d-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcatgctcag ggcagggctg 2341 tgcccgggcc tcaccgagga gaccgtccag cttctcagag gccgaaagat aaaaacagtg 2401 gcagacctgg cagctgctga cttggaggaa gtagcccaga agtgtggctt gtcctacaag 2461 gccctcgttg ccctgaggag ggtgttgctg gcgcagttct cggctttccc cttaaatggc 2521 gcagatctct atgaggaact gaagacttcc acggccatcc tgtccaccgg catcggaagc 2581 ctggacaaac tacttgatgc tggcctctat actggggagg tgactgaaat tgtgggtggc 2641 ccaggtagcg gcaaaaccca ggtgtgtctc tgtgtggctg caaatgtggc ccatagcctg 2701 cagcagaatg tactgtatgt ggattccaat ggaggaatga cggcgtcccg cctcctccag 2761 ctactacagg ctagaaccca agatgaggag aaacaggcaa gtgctctcca gaggatacag 2821 gtggtgcgtt catttgacat cttccggatg ctagatatgc tacaggacct tcgcggcacc 2881 atagcccagc aggaagcaac ttcttcaggc gccgtgaagg ttgtgattgt ggactcggtc 2941 actgcagtgg tcgccccact tctgggaggt cagcagaggg aaggcctggc cttgatgatg 3001 cagctggccc gagagctcaa gatcctggcc cgggacctgg gtgtggcagt ggtggtgacc 3061 aaccacttga ctcgagattg ggatggtaga agattcaaac ctgcccttgg acgctcctgg 3121 agctttgtgc ccagtacccg gattctcctg gatgtcactg agggggctgg gacactcggt 3181 agcagccaac gcacagtatg tctgaccaag tctccccgcc agccaacggg tctgcaggag 3241 atgatagaca ttgggacatt ggggactgag gagcagagcc cagaattacc tggcaagcag 3301 acgggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 3361 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 3421 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3481 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3541 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3601 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 3661 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 3721 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 3781 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 3841 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 3901 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 3961 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 4021 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 4081 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 4141 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 4201 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 4261 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 4321 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 4381 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 4441 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4501 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 4561 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 4621 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 4681 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 4741 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4801 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4861 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4921 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4981 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5041 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5101 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5161 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5221 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5281 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5341 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 5401 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 5461 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 5521 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 5581 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 5641 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 5701 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 5761 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 5821 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 5881 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 5941 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 6001 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 6061 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 6121 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6181 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6241 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 6361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 6481 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6601 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 6661 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6721 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6781 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6841 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7021 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7681 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7741 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7801 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7861 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7921 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7981 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8041 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8101 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8161 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 8221 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8281 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8341 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8401 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8461 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8521 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8581 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8641 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8701 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8761 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 8821 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 8881 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 8941 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 9001 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 9061 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 9121 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 9181 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 9241 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.