pLV3-CMV-gene-ATG9A-3×HA-Blast

pLV3-CMV-gene-ATG9A-3×HA-Blast plasmid

PVT100722

pLV3-CMV-gene-ATG9A-3×HA-Blast plasmid Information

Plasmid name: pLV3-CMV-gene-ATG9A(human)-3×HA-Blast
Alias: NM_001077198.3;APG9L1; mATG9; MGD3208
Gene lenght: 2517bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-gene-ATG9A-3×HA-Blast plasmid Description

pLV3-CMV-gene-ATG9A-3×HA-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-gene-ATG9A-3×HA-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggaccct agcaaagact caaaagctca ggtgtccgct 2341 gccgaggctg gcattactgg aacatggtac aatcagctcg ggagcacctt tattgtgact 2401 gctggagccg atggagccct caccggaaca tacgaatctg ctgtgggaaa cgccgaatca 2461 cggtacgtcc tcactggccg atacgatagt gcccctgcca ccgacggatc tgggactgcc 2521 ctgggatgga ctgtcgcttg gaaaaacaac taccggaatg ctcattctgc cacaacatgg 2581 agtggacagt acgtgggagg cgctgaggct agaatcaata cacagtggct gctcacatct 2641 ggcacaaccg aggcaaatgc ttggaaatcc accctggtgg gacatgacac attcaccaaa 2701 gtgaaaccct ccgccgcttc aatcgatgcc gccaaaaaag ccggagtcaa caacggcaat 2761 cctctggatg ccgtccagca gggtagcggc agcggtagca tggcgcagtt tgacactgaa 2821 taccagcgcc tagaggcctc ctatagtgat tcacccccag gggaggagga cctgttggtg 2881 cacgtcgccg aggggagcaa gtcaccttgg caccatattg aaaaccttga cctcttcttc 2941 tctcgagttt ataatctgca ccagaagaat ggcttcacat gtatgctcat cggggagatc 3001 tttgagctca tgcagttcct ctttgtggtt gccttcacta ccttcctggt cagctgcgtg 3061 gactatgaca tcctatttgc caacaagatg gtgaaccaca gtcttcaccc tactgaaccc 3121 gtcaaggtca ctctgccaga cgcctttttg cctgctcaag tctgtagtgc caggattcag 3181 gaaaatggct cccttatcac catcctggtc attgctggtg tcttctggat ccaccggctt 3241 atcaagttca tctataacat ttgctgctac tgggagatcc actccttcta cctgcacgct 3301 ctgcgcatcc ctatgtctgc ccttccgtat tgcacgtggc aagaagtgca ggcccggatc 3361 gtgcagacgc agaaggagca ccagatctgc atccacaaac gtgagctgac agaactggac 3421 atctaccacc gcatcctccg tttccagaac tacatggtgg cactggttaa caaatccctc 3481 ctgcctctgc gcttccgcct gcctggcctc ggggaagctg tcttcttcac ccgtggtctc 3541 aagtacaact ttgagctgat cctcttctgg ggacctggct ctctgtttct caatgaatgg 3601 agcctcaagg ccgagtacaa acgtgggggg caacggctag agctggccca gcgcctcagc 3661 aaccgcatcc tgtggattgg catcgctaac ttcctgctgt gccccctcat cctcatatgg 3721 caaatcctct atgccttctt cagctatgct gaggtgctga agcgggagcc gggggccctg 3781 ggagcacgct gctggtcact ctatggccgc tgctacctcc gccacttcaa cgagctggag 3841 cacgagctgc agtcccgcct caaccgtggc tacaagcccg cctccaagta catgaattgc 3901 ttcttgtcac ctcttttgac actgctggcc aagaatggag ccttcttcgc tggctccatc 3961 ctggctgtgc ttattgccct caccatttat gacgaagatg tgttggctgt ggaacatgtg 4021 ctgaccaccg tcacactcct gggggtcacc gtgaccgtgt gcaggtcctt tatcccggac 4081 cagcacatgg tgttctgccc tgagcagctg ctccgcgtga tcctcgctca catccactac 4141 atgcctgacc actggcaggg taatgcccac cgctcgcaga cccgggacga gtttgcccag 4201 ctcttccagt acaaggcagt gttcattttg gaagagttgc tgagccccat tgtcacaccc 4261 ctcatcctca tcttctgcct gcgcccacgg gccctggaga ttatagactt cttccgaaac 4321 ttcaccgtgg aggtcgttgg tgtgggagat acctgctcct ttgctcagat ggatgttcgc 4381 cagcatggtc atccccagtg gctatctgct gggcagacag aggcctcagt gtaccagcaa 4441 gctgaggatg gaaagacaga gttgtcactc atgcactttg ccatcaccaa ccctggctgg 4501 cagccaccac gtgagagcac agccttccta ggcttcctca aggagcaggt tcagcgggat 4561 ggagcagctg ctagcctcgc ccaagggggt ctgctccctg aaaatgccct ctttacgtct 4621 atccagtcct tacaatctga gtctgagccc ctgagcctta tcgcaaatgt ggtagctggc 4681 tcatcctgcc ggggccctcc actgcccaga gacctgcagg gctccaggca cagggctgaa 4741 gtcgcctctg ccctgcgctc cttctccccg ctgcaacccg ggcaggcgcc cacaggccgg 4801 gctcacagca ccatgacagg ctctggggtg gatgccagga cagccagctc cgggagcagc 4861 gtgtgggaag gacagctgca gagcctggtg ctgtcagaat atgcatccac agagatgagc 4921 ctgcatgccc tctatatgca ccagctccac aagcagcagg cccaggctga acctgagcgg 4981 catgtatggc accgccggga gagtgatgag agtggagaaa gcgcccctga tgaaggggga 5041 gagggcgccc gggcccccca gtctatccct cgctctgcta gctatccctg tgcagcaccc 5101 cggcctggag ctcctgagac cactgccctg catgggggct tccagaggcg ctacggtggc 5161 atcacagatc ctggcacagt gcccagggtt ccctctcatt tctctcggct gcctcttgga 5221 gggtgggcag aagatgggca gtcggcatca aggcaccctg agcccgtgcc cgaagagggc 5281 tcggaggatg agctaccccc tcaggtgcac aaggtaCTCG AGTACCCATA CGACGTACCT 5341 GATTATGCAA CTTATCCTTA TGATGTACCT GATTATGCTA GCTATCCTTA TGATGTCCCA 5401 GACTACGCAT AGGGATCCGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG 5461 GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC 5521 GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG 5581 CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT 5641 TTTTCGCAAC GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC 5701 TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC 5761 GCCTCCCGCC TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG 5821 GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC 5881 CACGCTTTGC CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG 5941 CCGTTACAGA TCCAAGCTGT GACCGGCGCC TACGCTAGGC CACCATGGCC AAGCCTTTGT 6001 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT 6061 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG 6121 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG 6181 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA 6241 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG 6301 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT 6361 CTGGTTATGT GTGGGAGGGC TAAGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA 6421 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 6481 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 6541 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 6601 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 6661 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 6721 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 6781 GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 6841 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 6901 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC 6961 TTTGGGCCGC CTCCCCGCCT GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT 7021 CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAAA 7081 TAAGATCTGC TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG 7141 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT 7201 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT 7261 TAGTCAGTGT GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA 7321 TAACTTGCAA AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC AGCTTATAAT 7381 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT 7441 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTAGCTATCC 7501 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 7561 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 7621 TTTTTGGAGG CCTAGACTTT TGCAGAGACG GCCCAAATTC GTAATCATGG TCATAGCTGT 7681 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 7741 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 7801 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 7861 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 7921 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 7981 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 8041 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 8101 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 8161 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 8221 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 8281 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 8341 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 8401 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 8461 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT 8521 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 8581 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 8641 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 8701 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 8761 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 8821 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 8881 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 8941 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 9001 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 9061 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 9121 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 9181 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 9241 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 9301 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 9361 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 9421 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 9481 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 9541 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 9601 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 9661 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 9721 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 9781 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA 9841 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT 9901 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 9961 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT 10021 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC 10081 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT 10141 TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC 10201 TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT 10261 CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.