pTac-GST-MYH9 plasmid

pTac-GST-MYH9 plasmid

PVT100331

pTac-GST-MYH9 plasmid Information

Plasmid name: pTac-GST-MYH9(human)
Alias: NM_002473.6;BDPLT6; DFNA17; EPSTS; FTNS; MATINS; MHA;NMHC-II-A; NMMHC-IIA; NMMHCA
Gene lenght: 5883bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-MYH9 plasmid Description

pTac-GST-MYH9 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-MYH9 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggc acagcaagct gccgataagt 961 atctctatgt ggataaaaac ttcatcaaca atccgctggc ccaggccgac tgggctgcca 1021 agaagctggt atgggtgcct tccgacaaga gtggctttga gccagccagc ctcaaggagg 1081 aggtgggcga agaggccatc gtggagctgg tggagaatgg gaagaaggtg aaggtgaaca 1141 aggatgacat ccagaagatg aacccgccca agttctccaa ggtggaggac atggcagagc 1201 tcacgtgcct caacgaagcc tcggtgctgc acaacctcaa ggagcgttac tactcagggc 1261 tcatctacac ctattcaggc ctgttctgtg tggtcatcaa tccttacaag aacctgccca 1321 tctactctga agagattgtg gaaatgtaca agggcaagaa gaggcacgag atgccccctc 1381 acatctatgc catcacagac accgcctaca ggagtatgat gcaagaccga gaagatcaat 1441 ccatcttgtg cactggtgaa tctggagctg gcaagacgga gaacaccaag aaggtcatcc 1501 agtatctggc gtacgtggcg tcctcgcaca agagcaagaa ggaccagggc gagctggagc 1561 ggcagctgct gcaggccaac cccatcctgg aggccttcgg gaacgccaag accgtgaaga 1621 atgacaactc ctcccgcttc ggcaaattca ttcgcatcaa ctttgatgtc aatggctaca 1681 ttgttggagc caacattgag acttatcttt tggagaaatc tcgtgctatc cgccaagcca 1741 aggaagaacg gaccttccac atcttctatt atctcctgtc tggggctgga gagcacctga 1801 agaccgatct cctgttggag ccgtacaaca aataccgctt cctgtccaat ggacacgtca 1861 ccatccccgg gcagcaggac aaggacatgt tccaggagac catggaggcc atgaggatta 1921 tgggcatccc agaagaggag caaatgggcc tgctgcgggt catctcaggg gttcttcagc 1981 tcggcaacat cgtcttcaag aaggagcgga acactgacca ggcgtccatg cccgacaaca 2041 cagctgccca aaaggtgtcc catctcttgg gtatcaatgt gaccgatttc accagaggaa 2101 tcctcacccc gcgcatcaag gtgggacggg attacgtcca gaaggcgcag actaaagagc 2161 aggctgactt tgccatcgag gccttggcca aggcgaccta tgagcggatg ttccgctggc 2221 tggtgctgcg catcaacaag gctctggaca agaccaagag gcagggcgcc tccttcatcg 2281 ggatcctgga cattgccggc ttcgagatct ttgatctgaa ctcgtttgag cagctgtgca 2341 tcaattacac caatgagaag ctgcagcagc tcttcaacca caccatgttc atcctggagc 2401 aggaggagta ccagcgcgag ggcatcgagt ggaacttcat cgactttggc ctcgacctgc 2461 agccctgcat cgacctcatt gagaagccag caggcccccc gggcattctg gccctgctgg 2521 acgaggagtg ctggttcccc aaagccaccg acaagagctt cgtggagaag gtgatgcagg 2581 agcagggcac ccaccccaag ttccagaagc ccaagcagct gaaggacaaa gctgatttct 2641 gcattatcca ctatgccggc aaggtggatt acaaagctga cgagtggctg atgaagaaca 2701 tggatcccct gaatgacaac atcgccacac tgctccacca gtcctctgac aagtttgtct 2761 cggagctgtg gaaggatgtg gaccgcatca tcggcctgga ccaggtggcc ggcatgtcgg 2821 agaccgcact gcccggggcc ttcaagacgc ggaagggcat gttccgcact gtggggcagc 2881 tttacaagga gcagctggcc aagctgatgg ctacgctgag gaacacgaac cccaactttg 2941 tccgctgcat catccccaac cacgagaaga aggccggcaa gctggacccg catctcgtgc 3001 tggaccagct gcgctgcaac ggtgttctcg agggcatccg tatctgccgc cagggcttcc 3061 ccaacagggt ggtcttccag gagtttcggc agagatatga gatcctgact ccaaactcca 3121 ttcccaaggg tttcatggac gggaagcagg cgtgcgtgct catgataaaa gccctggagc 3181 tcgacagcaa tctgtaccgc attggccaga gcaaagtctt cttccgtgcc ggtgtgctgg 3241 cccacctgga ggaggagcga gacctgaaga tcaccgacgt catcataggg ttccaggcct 3301 gctgcagggg ctacctggcc aggaaagcat ttgccaagcg gcagcagcag cttaccgcca 3361 tgaaggtcct ccagcggaac tgcgctgcct acctgaagct gcggaactgg cagtggtggc 3421 ggctcttcac caaggtcaag ccgctgctgc aggtgagccg gcaggaggag gagatgatgg 3481 ccaaggagga ggagctggtg aaggtcagag agaagcagct ggctgcggag aacaggctca 3541 cggagatgga gacgctgcag tctcagctca tggcagagaa attgcagctg caggagcagc 3601 tccaggcaga aaccgagctg tgtgccgagg ctgaggagct ccgggcccgc ctgaccgcca 3661 agaagcagga attagaagag atctgccatg acctagaggc cagggtggag gaggaggagg 3721 agcgctgcca gcacctgcag gcggagaaga agaagatgca gcagaacatc caggagcttg 3781 aggagcagct ggaggaggag gagagcgccc ggcagaagct gcagctggag aaggtgacca 3841 ccgaggcgaa gctgaaaaag ctggaggagg agcagatcat cctggaggac cagaactgca 3901 agctggccaa ggaaaagaaa ctgctggaag acagaatagc tgagttcacc accaacctca 3961 cagaagagga ggagaaatct aagagcctcg ccaagctcaa gaacaagcat gaggcaatga 4021 tcactgactt ggaagagcgc ctccgcaggg aggagaagca gcgacaggag ctggagaaga 4081 cccgccggaa gctggaggga gactccacag acctcagcga ccagatcgcc gagctccagg 4141 cccagatcgc ggagctcaag atgcagctgg ccaagaaaga ggaggagctc caggccgccc 4201 tggccagagt ggaagaggaa gctgcccaga agaacatggc cctcaagaag atccgggagc 4261 tggaatctca gatctctgaa ctccaggaag acctggagtc tgagcgtgct tccaggaata 4321 aagctgagaa gcagaaacgg gaccttgggg aagagctaga ggctctgaaa acagagttgg 4381 aggacacgct ggattccaca gctgcccagc aggagctcag gtcaaaacgt gagcaggagg 4441 tgaacatcct gaagaagacc ctggaggagg aggccaagac ccacgaggcc cagatccagg 4501 agatgaggca gaagcactca caggccgtgg aggagctggc ggagcagctg gagcagacga 4561 agcgggtgaa agcaaacctc gagaaggcaa agcagactct ggagaacgag cggggggagc 4621 tggccaacga ggtgaaggtg ctgctgcagg gcaaagggga ctcggagcac aagcgcaaga 4681 aagtggaggc gcagctgcag gagctgcagg tcaagttcaa cgagggagag cgcgtgcgca 4741 cagagctggc cgacaaggtc accaagctgc aggtggagct ggacaacgtg accgggcttc 4801 tcagccagtc cgacagcaag tccagcaagc tcaccaagga cttctccgcg ctggagtccc 4861 agctgcagga cactcaggag ctgctgcagg aggagaaccg gcagaagctg agcctgagca 4921 ccaagctcaa gcaggtggag gacgagaaga attccttccg ggagcagctg gaggaggagg 4981 aggaggccaa gcacaacctg gagaagcaga tcgccaccct ccatgcccag gtggccgaca 5041 tgaaaaagaa gatggaggac agtgtggggt gcctggaaac tgctgaggag gtgaagagga 5101 agctccagaa ggacctggag ggcctgagcc agcggcacga ggagaaggtg gccgcctacg 5161 acaagctgga gaagaccaag acgcggctgc agcaggagct ggacgacctg ctggtggacc 5221 tggaccacca gcgccagagc gcgtgcaacc tggagaagaa gcagaagaag tttgaccagc 5281 tcctggcgga ggagaagacc atctctgcca agtatgcaga ggagcgcgac cgggctgagg 5341 cggaggcccg agagaaggag accaaggctc tgtcgctggc ccgggccctg gaggaagcca 5401 tggagcagaa ggcggagctg gagcggctca acaagcagtt ccgcacggag atggaggacc 5461 ttatgagctc caaggatgat gtgggcaaga gtgtccacga gctggagaag tccaagcggg 5521 ccctagagca gcaggtggag gagatgaaga cgcagctgga agagctggag gacgagctgc 5581 aggccaccga agatgccaag ctgcggttgg aggtcaacct gcaggccatg aaggcccagt 5641 tcgagcggga cctgcagggc cgggacgagc agagcgagga gaagaagaag cagctggtca 5701 gacaggtgcg ggagatggag gcagagctgg aggacgagag gaagcagcgc tcgatggcag 5761 tggccgcccg gaagaagctg gagatggacc tgaaggacct ggaggcgcac atcgactcgg 5821 ccaacaagaa ccgggacgaa gccatcaaac agctgcggaa gctgcaggcc cagatgaagg 5881 actgcatgcg cgagctggat gacacccgcg cctctcgtga ggagatcctg gcccaggcca 5941 aagagaacga gaagaagctg aagagcatgg aggccgagat gatccagttg caggaggaac 6001 tggcagccgc ggagcgtgcc aagcgccagg cccagcagga gcgggatgag ctggctgacg 6061 agatcgccaa cagcagcggc aaaggagccc tggcgttaga ggagaagcgg cgtctggagg 6121 cccgcatcgc ccagctggag gaggagctgg aggaggagca gggcaacacg gagctgatca 6181 acgaccggct gaagaaggcc aacctgcaga tcgaccagat caacaccgac ctgaacctgg 6241 agcgcagcca cgcccagaag aacgagaatg ctcggcagca gctggaacgc cagaacaagg 6301 agcttaaggt caagctgcag gagatggagg gcactgtcaa gtccaagtac aaggcctcca 6361 tcaccgccct cgaggccaag attgcacagc tggaggagca gctggacaac gagaccaagg 6421 agcgccaggc agcctgcaaa caggtgcgtc ggaccgagaa gaagctgaag gatgtgctgc 6481 tgcaggtgga tgacgagcgg aggaacgccg agcagtacaa ggaccaggcc gacaaggcat 6541 ctacccgcct gaagcagctc aagcggcagc tggaggaggc cgaagaggag gcccagcggg 6601 ccaacgcctc ccgccggaaa ctgcagcgcg agctggagga cgccactgag acggccgatg 6661 ccatgaaccg cgaagtcagc tccctaaaga acaagctcag gcgcggggac ctgccgtttg 6721 tcgtgccccg ccgaatggcc cggaaaggcg ccggggatgg ctccgacgaa gaggtagatg 6781 gcaaagcgga tggggctgag gccaaacctg ccgaataact cgagcggccg catcgtgact 6841 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 6901 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 6961 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 7021 gcggagtgta taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta 7081 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 7141 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 7201 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 7261 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 7321 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 7381 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 7441 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag 7501 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 7561 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 7621 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 7681 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 7741 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa 7801 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 7861 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 7921 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 7981 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 8041 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 8101 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 8161 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 8221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 8281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 8341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 8401 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 8461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 8521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 8581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 8641 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 8701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 8761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 8821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 8881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 8941 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 9001 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt 9061 ttctccttac gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggt 9121 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga 9181 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg 9241 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag 9301 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac 9361 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg 9421 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag 9481 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca 9541 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct 9601 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta 9661 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc 9721 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg 9781 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact 9841 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca 9901 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt 9961 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct 10021 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca 10081 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc 10141 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc 10201 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 10261 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 10321 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 10381 aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga 10441 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 10501 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 10561 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga 10621 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc 10681 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga 10741 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg 10801 ccagacgcga attatttttg atggcgttgg aatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.