pCMV-WWP2-3×Myc-Neo plasmid

pCMV-WWP2-3×Myc-Neo plasmid

PVT100122

pCMV-WWP2-3×Myc-Neo plasmid Information

Plasmid name: pCMV-WWP2(human)-3×Myc-Neo
Alias: NM_001270454.2;AIP2; WWp2-like
Gene lenght: 2610bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-WWP2-3×Myc-Neo plasmid Description

pCMV-WWP2-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-WWP2-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc atctgccagc 721 tctagccggg caggagtggc cctgcctttt gagaagtctc agctcacttt gaaagtggtg 781 tccgcaaagc ccaaggtgca taatcgtcaa cctcgaatta actcctacgt ggaggtggcg 841 gtggatggac tccccagtga gaccaagaag actgggaagc gcattgggag ctctgagctt 901 ctctggaatg agatcatcat tttgaatgtc acggcacaga gtcatttaga tttaaaggtc 961 tggagctgcc ataccttgag aaatgaactg ctaggcaccg catctgtcaa cctctccaac 1021 gtcttgaaga acaatggggg caaaatggag aacatgcagc tgaccctgaa cctgcagacg 1081 gagaacaaag gcagcgttgt ctcaggcgga gagctgacaa ttttcctgga cgggccaact 1141 gttgatctgg gaaatgtgcc taatggcagt gccctgacag atggatcaca gctgccttcg 1201 agagactcca gtggaacagc agtagctcca gagaaccggc accagccccc cagcacaaac 1261 tgctttggtg gaagatcccg gacgcacaga cattcgggtg cttcagccag aacaacccca 1321 gcaaccggcg agcaaagccc cggtgctcgg agccggcacc gccagcccgt caagaactca 1381 ggccacagtg gcttggccaa tggcacagtg aatgatgaac ccacaacagc cactgatccc 1441 gaagaacctt ccgttgttgg tgtgacgtcc ccacctgctg cacccttgag tgtgaccccg 1501 aatcccaaca cgacttctct ccctgcccca gccacaccgg ctgaaggaga ggaacccagc 1561 acttcgggta cacagcagct cccagcggct gcccaggccc ccgacgctct gcctgctgga 1621 tgggaacagc gagagctgcc caacggacgt gtctattatg ttgaccacaa taccaagacc 1681 accacctggg agcggcccct tcctccaggc tgggaaaaac gcacagatcc ccgaggcagg 1741 ttttactatg tggatcacaa tactcggacc accacctggc agcgtccgac cgcggagtac 1801 gtgcgcaact atgagcagtg gcagtcgcag cggaatcagc tccagggggc catgcagcac 1861 ttcagccaaa gattcctcta ccagtcttcg agtgcttcga ctgaccatga tcccctgggc 1921 cccctccctc ctggctggga gaagagacag gacaatggac gggtgtatta cgtgaaccat 1981 aacactcgca cgacccagtg ggaggatccc cggacccagg ggatgatcca ggaaccagct 2041 ctgcccccag gatgggagat gaaatacacc agcgaggggg tgcgatactt tgtggaccac 2101 aatacccgca ccaccacctt taaggatcct cgcccggggt ttgagtcggg gacgaagcaa 2161 ggttcccctg gtgcttatga ccgcagtttt cggtggaagt atcaccagtt ccgtttcctc 2221 tgccattcaa atgccctacc tagccacgtg aagatcagcg tttccaggca gacgcttttc 2281 gaagattcct tccaacagat catgaacatg aaaccctatg acctgcgccg ccggctctac 2341 atcatcatgc gtggcgagga gggcctggac tatgggggca tcgccagaga gtggtttttc 2401 ctcctgtctc atgaggtgct caaccctatg tattgtttat ttgaatatgc cggaaagaac 2461 aattactgcc tgcagatcaa ccccgcctcc tccatcaacc cggaccacct cacctacttt 2521 cgctttatag gcagattcat cgccatggcg ctgtaccatg gaaagttcat cgacacgggc 2581 ttcaccctcc ctttctacaa gcggatgctc aataagagac caaccctgaa agacctggag 2641 tccattgacc ctgagttcta caactccatt gtctggatca aagagaacaa cctggaagaa 2701 tgtggcctgg agctgtactt catccaggac atggagatac tgggcaaggt gacgacccac 2761 gagctgaagg agggcggcga gagcatccgg gtcacagagg agaacaagga agagtacatc 2821 atgctgctga ctgactggcg tttcacccga ggcgtggaag agcagaccaa agccttcctg 2881 gatggcttca acgaggtggc cccgctggag tggctgcgct actttgacga gaaagagctg 2941 gagctgatgc tgtgcggcat gcaggagata gacatgagcg actggcagaa gagcaccatc 3001 taccggcact acaccaagaa cagcaagcag atccagtggt tctggcaggt ggtgaaggag 3061 atggacaacg agaagaggat ccggctgctg cagtttgtca ccggtacctg ccgcctgccc 3121 gtcgggggat ttgccgaact catcggtagc aacggaccac agaagttttg cattgacaaa 3181 gttggcaagg aaacctggct gcccagaagc cacacctgct tcaaccgtct ggatcttcca 3241 ccctacaaga gctacgaaca gctgagagag aagctgctgt atgccattga ggagaccgag 3301 ggctttggac aggagCTCGA GGAACAAAAA CTAATATCAG AGGAAGATTT GGAACAGAAA 3361 CTAATCAGTG AAGAAGATTT AGAGCAAAAG TTAATTTCCG AGGAGGACTT ATAGTGAGGG 3421 CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC 3481 TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG 3541 CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC 3601 TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG 3661 ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG 3721 CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA 3781 CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT 3841 TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG 3901 TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC 3961 CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC 4021 CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG 4081 CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA 4141 AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG 4201 GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC 4261 CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG 4321 GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 4381 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 4441 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC 4501 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 4561 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 4621 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 4681 TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC 4741 TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 4801 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 4861 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 4921 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 4981 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 5041 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 5101 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG 5161 ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG 5221 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC 5281 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG 5341 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 5401 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG 5461 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT 5521 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA 5581 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA 5641 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 5701 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA 5761 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC 5821 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA 5881 ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC 5941 CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG 6001 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 6061 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 6121 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 6181 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 6241 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 6301 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 6361 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 6421 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 6481 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 6541 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 6601 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 6661 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 6721 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 6781 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 6841 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 6901 TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 6961 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 7021 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 7081 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 7141 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 7201 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT 7261 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 7321 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 7381 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 7441 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 7501 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 7561 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 7621 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 7681 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 7741 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 7801 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 7861 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 7921 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 7981 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 8041 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 8101 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 8161 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 8221 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 8281 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 8341 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 8401 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 8461 TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC 8521 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT 8581 CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG 8641 CATGAAGAAT CTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.