pLV2-TRE3GS-PE6c-TetOne-Blast

pLV2-TRE3GS-PE6c-TetOne-Blast plasmid

PVT100081

pLV2-TRE3GS-PE6c-TetOne-Blast plasmid Information

Plasmid name: pLV2-TRE3GS-PE6c-TetOne-Blast
Alias: -
Gene lenght: 5880bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: TRE3G
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-PE6c-TetOne-Blast plasmid Description

pLV2-TRE3GS-PE6c-TetOne-Blast is a plasmid suitable for protein expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-PE6c-TetOne-Blast plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG 4141 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA 4201 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA 4261 CGATACAAGT CAGGTTGCCA GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG 4321 CACAAGGTCC CCCAGTAAAA TGATATACAT TGACACCAGT GAAGATGCGG CCGTCGCTAG 4381 AGAGAGCTGC GCTGGCGACG CTGTAGTCTT CAGAGATGGG GATGCTGTTG ATTGTAGCCG 4441 TTGCTCTTTC AATGAGGGTG GATTCTTCTT GAGACAAAGG CTTGGCCATG GTGGCggtcg 4501 cgttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga 4561 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga 4621 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt 4681 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt 4741 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga 4801 gcctggggac tttccacacc ctaactgaca cacattcgcg cgtttacccg gggagcatgt 4861 caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg 4921 catcggctgg gagcatgtct aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt 4981 cgcactttag ctgtttctcc aggccacata tgattagttc caggccgaaa aggaaggcag 5041 gttcggctcc ctgccggtcg aacagctcaa ttgcttgttt cagaagtggg ggcatagaat 5101 cggtggtagg tgtctctctt tcctcttttg ctacttgatg ctcctgttcc tccaatacgc 5161 agcccagtgt aaagtggccc acggcggaca gagcgtacag tgcgttctcc agggagaagc 5221 cttgctgaca caggaacgcg agctgatttt ccagggtttc gtactgtttc tctgttgggc 5281 gggtgccgag atgcacttta gccccgtcgc gatgtgagag gagagcacag cggtatgact 5341 tggcgttgtt ccgcagaaag tcttgccatg actcgccttc cagggggcag gagtgggtat 5401 gatgcctgtc cagcatctcg attggcaggg catcgagcag ggcccgcttg ttcttcacgt 5461 gccagtacag ggtaggctgc tcaactccca gcttttgagc gagtttcctt gtcgtcaggc 5521 cttcgatacc gactccattg agtaattcca gagcagagtt tatgactttg ctcttgtcca 5581 gtctagacat ggtaattcga tgatccccct ggggagagag gtcggtgatt cggtcaacga 5641 gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac cgcgcgggca 5701 ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct tcccggccgc 5761 tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc ggtcggcgcg 5821 ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt gcggcttccg 5881 tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg ggcggagcag 5941 gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg ctgcgaacgg 6001 acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa ccacgcccag 6061 agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac cccaaccccg 6121 tggaattatc acctcgagtt tactccctat cagtgataga gaacgtatga agagtttact 6181 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat 6241 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga 6301 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact 6361 ccctatcagt gatagagaac gtataagctt tgcttatgta aaccagggcg cctataaaag 6421 agtgctgatt ttttgagtaa acttcaattc cacaacactt ttgtcttata ccaactttcc 6481 gtaccacttc ctaccctcgt aaagaattcc cgtatacacc ggtGCCACCA TGAAACGGAC 6541 AGCCGACGGA AGCGAGTTCG AGTCACCAAA GAAGAAGCGG AAAGTCGACA AGAAGTACAG 6601 CATCGGCCTG GACATCGGCA CCAACTCTGT GGGCTGGGCC GTGATCACCG ACGAGTACAA 6661 GGTGCCCAGC AAGAAATTCA AGGTGCTGGG CAACACCGAC CGGCACAGCA TCAAGAAGAA 6721 CCTGATCGGA GCCCTGCTGT TCGACAGCGG CGAAACAGCC GAGGCCACCC GGCTGAAGAG 6781 AACCGCCAGA AGAAGATACA CCAGACGGAA GAACCGGATC TGCTATCTGC AAGAGATCTT 6841 CAGCAACGAG ATGGCCAAGG TGGACGACAG CTTCTTCCAC AGACTGGAAG AGTCCTTCCT 6901 GGTGGAAGAG GATAAGAAGC ACGAGCGGCA CCCCATCTTC GGCAACATCG TGGACGAGGT 6961 GGCCTACCAC GAGAAGTACC CCACCATCTA CCACCTGAGA AAGAAACTGG TGGACAGCAC 7021 CGACAAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG 7081 CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT 7141 CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT 7201 GGACGCCAAG GCCATCCTGT CTGCCAGACT GAGCAAGAGC AGAAAGCTGG AAAATCTGAT 7261 CGCCCAGCTG CCCGGCGAGA AGAAGAATGG CCTGTTCGGA AACCTGATTG CCCTGAGCCT 7321 GGGCCTGACC CCCAACTTCA AGAGCAACTT CGACCTGGCC GAGGATGCCA AACTGCAGCT 7381 GAGCAAGGAC ACCTACGACG ACGACCTGGA CAACCTGCTG GCCCAGATCG GCGACCAGTA 7441 CGCCGACCTG TTTCTGGCCG CCAAGAACCT GTCCGACGCC ATCCTGCTGA GCGACATCCT 7501 GAGAGTGAAC ACCGAGATCA CCAAGGCCCC CCTGAGCGCC TCTATGATCA AGAGATACGA 7561 CGAGCACCAC CAGGACCTGA CCCTGCTGAA AGCTCTCGTG CGGCAGCAGC TGCCTGAGAA 7621 GTACAAAGAG ATTTTCTTCG ACCAGAGCAA GAACGGCTAC GCCGGCTACA TTGACGGCGG 7681 AGCCAGCCAG GAAGAGTTCT ACAAGTTCAT CAAGCCCATC CTGGAAAAGA TGGACGGCAC 7741 CGAGGAACTG CTCGTGAAGC TGAAGAGAGA GGACCTGCTG CGGAAGCAGC GGACCTTCGA 7801 CAACGGCAGC ATCCCCCACC AGATCCACCT GGGAGAGCTG CACGCCATTC TGCGGCGGCA 7861 GGAAGATTTT TACCCATTCC TGAAGGACAA CCGGGAAAAG ATCGAGAAGA TCCTGACCTT 7921 CCGCATCCCC TACTACGTGG GCCCTCTGGC CAGGGGAAAC AGCAGATTCG CCTGGATGAC 7981 CAGAAAGAGC GAGGAAACCA TCACCCCCTG GAACTTCGAG GAAGTGGTGG ACAAGGGCGC 8041 TTCCGCCCAG AGCTTCATCG AGCGGATGAC CAACTTCGAT AAGAACCTGC CCAACGAGAA 8101 GGTGCTGCCC AAGCACAGCC TGCTGTACGA GTACTTCACC GTGTATAACG AGCTGACCAA 8161 AGTGAAATAC GTGACCGAGG GAATGAGAAA GCCCGCCTTC CTGAGCGGCG AGCAGAAAAA 8221 GGCCATCGTG GACCTGCTGT TCAAGACCAA CCGGAAAGTG ACCGTGAAGC AGCTGAAAGA 8281 GGACTACTTC AAGAAAATCG AGTGCTTCGA CTCCGTGGAA ATCTCCGGCG TGGAAGATCG 8341 GTTCAACGCC TCCCTGGGCA CATACCACGA TCTGCTGAAA ATTATCAAGG ACAAGGACTT 8401 CCTGGACAAT GAGGAAAACG AGGACATTCT GGAAGATATC GTGCTGACCC TGACACTGTT 8461 TGAGGACAGA GAGATGATCG AGGAACGGCT GAAAACCTAT GCCCACCTGT TCGACGACAA 8521 AGTGATGAAG CAGCTGAAGC GGCGGAGATA CACCGGCTGG GGCAGGCTGA GCCGGAAGCT 8581 GATCAACGGC ATCCGGGACA AGCAGTCCGG CAAGACAATC CTGGATTTCC TGAAGTCCGA 8641 CGGCTTCGCC AACAGAAACT TCATGCAGCT GATCCACGAC GACAGCCTGA CCTTTAAAGA 8701 GGACATCCAG AAAGCCCAGG TGTCCGGCCA GGGCGATAGC CTGCACGAGC ACATTGCCAA 8761 TCTGGCCGGC AGCCCCGCCA TTAAGAAGGG CATCCTGCAG ACAGTGAAGG TGGTGGACGA 8821 GCTCGTGAAA GTGATGGGCC GGCACAAGCC CGAGAACATC GTGATCGAAA TGGCCAGAGA 8881 GAACCAGACC ACCCAGAAGG GACAGAAGAA CAGCCGCGAG AGAATGAAGC GGATCGAAGA 8941 GGGCATCAAA GAGCTGGGCA GCCAGATCCT GAAAGAACAC CCCGTGGAAA ACACCCAGCT 9001 GCAGAACGAG AAGCTGTACC TGTACTACCT GCAGAATGGG CGGGATATGT ACGTGGACCA 9061 GGAACTGGAC ATCAACCGGC TGTCCGACTA CGATGTGGAC GCTATCGTGC CTCAGAGCTT 9121 TCTGAAGGAC GACTCCATCG ACAACAAGGT GCTGACCAGA AGCGACAAGA ACCGGGGCAA 9181 GAGCGACAAC GTGCCCTCCG AAGAGGTCGT GAAGAAGATG AAGAACTACT GGCGGCAGCT 9241 GCTGAACGCC AAGCTGATTA CCCAGAGAAA GTTCGACAAT CTGACCAAGG CCGAGAGAGG 9301 CGGCCTGAGC GAACTGGATA AGGCCGGCTT CATCAAGAGA CAGCTGGTGG AAACCCGGCA 9361 GATCACAAAG CACGTGGCAC AGATCCTGGA CTCCCGGATG AACACTAAGT ACGACGAGAA 9421 TGACAAGCTG ATCCGGGAAG TGAAAGTGAT CACCCTGAAG TCCAAGCTGG TGTCCGATTT 9481 CCGGAAGGAT TTCCAGTTTT ACAAAGTGCG CGAGATCAAC AACTACCACC ACGCCCACGA 9541 CGCCTACCTG AACGCCGTCG TGGGAACCGC CCTGATCAAA AAGTACCCTA AGCTGGAAAG 9601 CGAGTTCGTG TACGGCGACT ACAAGGTGTA CGACGTGCGG AAGATGATCG CCAAGAGCGA 9661 GCAGGAAATC GGCAAGGCTA CCGCCAAGTA CTTCTTCTAC AGCAACATCA TGAACTTTTT 9721 CAAGACCGAG ATTACCCTGG CCAACGGCGA GATCCGGAAG CGGCCTCTGA TCGAGACAAA 9781 CGGCGAAACC GGGGAGATCG TGTGGGATAA GGGCCGGGAT TTTGCCACCG TGCGGAAAGT 9841 GCTGAGCATG CCCCAAGTGA ATATCGTGAA AAAGACCGAG GTGCAGACAG GCGGCTTCAG 9901 CAAAGAGTCT ATCCTGCCCA AGAGGAACAG CGATAAGCTG ATCGCCAGAA AGAAGGACTG 9961 GGACCCTAAG AAGTACGGCG GCTTCGACAG CCCCACCGTG GCCTATTCTG TGCTGGTGGT 10021 GGCCAAAGTG GAAAAGGGCA AGTCCAAGAA ACTGAAGAGT GTGAAAGAGC TGCTGGGGAT 10081 CACCATCATG GAAAGAAGCA GCTTCGAGAA GAATCCCATC GACTTTCTGG AAGCCAAGGG 10141 CTACAAAGAA GTGAAAAAGG ACCTGATCAT CAAGCTGCCT AAGTACTCCC TGTTCGAGCT 10201 GGAAAACGGC CGGAAGAGAA TGCTGGCCTC TGCCGGCGAA CTGCAGAAGG GAAACGAACT 10261 GGCCCTGCCC TCCAAATATG TGAACTTCCT GTACCTGGCC AGCCACTATG AGAAGCTGAA 10321 GGGCTCCCCC GAGGATAATG AGCAGAAACA GCTGTTTGTG GAACAGCACA AGCACTACCT 10381 GGACGAGATC ATCGAGCAGA TCAGCGAGTT CTCCAAGAGA GTGATCCTGG CCGACGCTAA 10441 TCTGGACAAA GTGCTGTCCG CCTACAACAA GCACCGGGAT AAGCCCATCA GAGAGCAGGC 10501 CGAGAATATC ATCCACCTGT TTACCCTGAC CAATCTGGGA GCCCCTGCCG CCTTCAAGTA 10561 CTTTGACACC ACCATCGACC GGAAGAGGTA CACCAGCACC AAAGAGGTGC TGGACGCCAC 10621 CCTGATCCAC CAGAGCATCA CCGGCCTGTA CGAGACACGG ATCGACCTGT CTCAGCTGGG 10681 AGGTGACTCC GGCGGAAGCT CTGGTGGCAG CAAGCGGACC GCCGACGGCT CTGAATTCGA 10741 GAGCCCTAAG AAGAAAAGAA AGGTGAGCGG AGGCTCTAGC GGCGGAAGCA TCAGCAGCTC 10801 TAAGCACACC CTGAGCCAGA TGAACAAGGT GAGCAACATC GTGAAGGAAC CCGAGCTGCC 10861 CGACATCTAC AAGGAATTTA AGGACATCAC CGCCGACACC AATACCGAGA AGCTGCCTAA 10921 GCCTATTAAG GGCCTGGAAT TTGAAGTGGA ACTGACACAG GAGAACTACA GACTGCCTAT 10981 CCGGAACTAT CCTCTGACTC CAGTCAAGAT GCAGGCCATG AACGACGAGA TCAATCAAGG 11041 CCTGAAAGGC GGCATCATCA GAGAGAGCAA GGCCATCAAC GCCTGCCCTG TTATATTCGT 11101 GCCCAGAAAG GAAGGCACAC TGCGCATGGT GGTCGACTAC AGGCCCCTGA ACAAGTACGT 11161 GAAGCCCAAC GTCTACCCCC TGCCACTGAT TGAACAACTG CTGGCCAAGA TCCAGGGCAG 11221 CACCATTTTC ACCAAGCTGG ACCTGAAAAG CGCCTACCAC CAGATCAGAG TGCGAAAGGG 11281 AGATGAGCAC AAGCTGGCCT TCCGGTGCCC CAGAGGAGTC TTCGAGTACC TGGTGATGCC 11341 TTACGGCATC AAGACAGCCC CTGCCCACTT TCAGTACTTC ATCAACACAA TCCTGGGCGA 11401 GGCCAAGGAA AGCCACGTGG TGTGCTACAT GGACGACATC CTGATCCATT CCAAGTCCGA 11461 GTCCGAACAC GTGAAACATG TGAAGGACGT GCTGCAAAAG CTGAAGAACG CTAATCTGAT 11521 CATCAACCAG GCCAAATGCG AGTTTCACCA GAGCCAAGTG AAGTTCCTGG GCTACCACAT 11581 CAGCGAGAAG GGCTTAACCC CATGTCAGGA GAACATCGAC AAGGTGCTGC AGTGGAAACA 11641 GCCTAAAAAC CAGAAGGAAC TGAGACAGTT CCTGGGCCAG GTGAACTACC TGAGAAAATT 11701 CATCCCCAAA ACCAGCCAGT TGACCCACCC TCTGAACAAA CTCCTGAAAA AGGATGTCAG 11761 ATGGAAATGG ACCCCTACCC AGACCCAGGC TATCGAGAAT ATCAAGCAAT GTCTGGTGTC 11821 TCCTCCTGTG CTGAGGCACT TCGACTTCAG CAAGAAGATC CTGCTTGAGA CAGACGTTTC 11881 TGATGTGGCC GTGGGAGCCG TGCTGAGCCA GAAGCATGAT GATGATAAGT ACTACCCTGT 11941 GGGCTATTAC AGCGCTAAAA TGAGCAAAGC CCAGCTGAAT TATTCTGTGT CCGACAAGGA 12001 GATGCTGGCT ATCATCAAAA GCCTGGAGCA CTGGCGGCAC TACCTGGAAT CTACAATCGA 12061 GCCCTTCAAG ATCCTGACCG ACCACAGAAA CCTGATTGGA AGAATCACAA ACGAGAGCGA 12121 ACCAGAGAAC AAGCGGCTGG CCAGATGGCA GCTGTTCCTG CAGGACTTCA ACTTCGAGAT 12181 CAACTACAGA CCTGGCTCTG CAAATCACAT CGCCGATGCC CTGTCTAGAA TCGTGGACGA 12241 GACTGAGCCT ATCCCTAAGG ACAACGAAGA TAACAGCATC AACTTCGTGA ACCAGATCAG 12301 CATCTCTGGC GGCTCAAAAA GAACCGCCGA CGGCAGCGAA TTCGAGTCTC CCAAGAAGAA 12361 GAGGAAAGTC GGCTCTGGCC CTGCCGCTAA GAGAGTGAAG CTGGACTAAg gatccagacc 12421 acctcccctg cgagctaagc tggacagcca atgacgggta agagagtgac atttttcact 12481 aacctaagac aggagggccg tcagagctac tgcctaatcc aaagacgggt aaaagtgata 12541 aaaatgtatc actccaacct aagacaggcg cagcttccga gggatttgag atccagacat 12601 gataagatac attgatgagt ttggacaaac caaaactaga atgcagtgaa aaaaatgcct 12661 tatttgtgaa atttgtgatg ctattgcctt atttgtaacc attataagct gcaataaaca 12721 agttaagtcg ataaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt 12781 ttgtaatttg tttttgtagt tctttagttt gtatgtctgt tgctattatg tctactattc 12841 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaggcgata ccgtcgagat 12901 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct 12961 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat 13021 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag 13081 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa 13141 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg 13201 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc 13261 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca 13321 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc 13381 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc 13441 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc 13501 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg 13561 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct 13621 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca 13681 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct 13741 ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt ttgttttgct 13801 cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac 13861 aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg 13921 atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat 13981 taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt 14041 tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa 14101 tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg 14161 tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc 14221 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct 14281 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa 14341 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg 14401 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt 14461 acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc 14521 ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa gctcgatatc 14581 agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat gctaggcggc 14641 tgtcaaacct ccactctaac acttctctct ccgggtcatc catcccatgc aggctcacag 14701 ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct ggctcaactg 14761 gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc cctggtgtgt 14821 agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt 14881 gtcttctttg ggagtgaatt agcccttcca

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.