pT3-EF1a-Lta plasmid

pT3-EF1a-Lta plasmid

PVT99907

pT3-EF1a-Lta plasmid Information

Plasmid name: pT3-EF1a-Lta(mouse)
Alias: NM_010735.2;hlb382; LT; LT-alpha; LT-[a]; LTalpha; Ltx; LT[a]; TNF-beta; Tnfb; TNFSF1; Tnfsf1b; Tnlg1e
Gene lenght: 609bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pT3-EF1a-Lta plasmid Description

pT3-EF1a-Lta is a plasmid suitable for protein expression in mammalian cells.

pT3-EF1a-Lta plasmid Sequence

1 GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG 61 GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT 121 GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA 181 GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC 241 GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT 301 ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG 361 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG 421 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT 481 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG 541 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC 601 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA 661 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT 721 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA 781 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG 841 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG 901 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT 961 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG 1021 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA 1081 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA 1141 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA 1201 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC 1261 TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG 1321 CGGCCGCCCC CTTCACCATG GGCCACCatg acactgctcg gccgtctcca cctcttgagg 1381 gtgcttggca cccctcctgt cttcctcctg gggctgctgc tggccctgcc tctaggggcc 1441 cagggactct ctggtgtccg cttctccgct gccaggacag cccatccact ccctcagaag 1501 cacttgaccc atggcatcct gaaacctgct gctcaccttg ttgggtaccc cagcaagcag 1561 aactcactgc tctggagagc aagcacggat cgtgcctttc tccgacatgg cttctctttg 1621 agcaacaact ccctcctgat ccccaccagt ggcctctact ttgtctactc ccaggtggtt 1681 ttctctggag aaagctgctc ccccagggcc attcccactc ccatctacct ggcacacgag 1741 gtccagctct tttcctccca ataccccttc catgtgcctc tcctcagtgc gcagaagtct 1801 gtgtatccgg gacttcaagg accgtgggtg cgctcaatgt accagggggc tgtgttcctg 1861 ctcagtaagg gagaccagct gtccacccac accgacggca tctcccatct acacttcagc 1921 cccagcagtg tattctttgg agcctttgca ctgtagTTTA AACCCGCTGA TCAGCCTCGA 1981 CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC 2041 TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC 2101 TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT 2161 GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA 2221 GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAATAACT 2281 TCGTATAATG TATGCTATAC GAAGTTATAT CGTATGCATG CTTTGCATAC TTCTGCCTGC 2341 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGAA TTCCTTGAAA 2401 TACATCCACA GGTACACCTC CAATTGACTC AAATGATGTC AATTAGTCTA TCAGAAGCTT 2461 CTAAAGCCAT GACATCATTT TCTGGAATTT TCCAAGCTGT TTAAAGGCAC AGTCAACTTA 2521 GTGTATGTAA ACTTCTGACC CACTGGAATT GTGATACAGT GAATTATAAG TGAAATAATC 2581 TGTCTGTAAA CAATTGTTGG AAAAATGACT TGTGTCATGC ACAAAGTAGA TGTCCTAACT 2641 GACTTGCCAA AACTATTGTT TGTTAACAAG AAATTTGTGG AGTAGTTGAA AAACGAGTTT 2701 TAATGACTCC AACTTAAGTG TATGTAAACT TCCGACTTCA ACTGTAGGGT ACCGAGCTCG 2761 AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 2821 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 2881 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT 2941 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC 3001 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA 3061 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC 3121 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA 3181 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT 3241 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG 3301 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT 3361 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT 3421 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA 3481 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC 3541 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC 3601 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG 3661 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA 3721 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC 3781 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT 3841 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG 3901 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT 3961 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC 4021 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT 4081 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC 4141 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC 4201 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT 4261 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG 4321 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC 4381 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA 4441 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG 4501 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA 4561 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA 4621 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG 4681 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG 4741 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG 4801 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG 4861 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC 4921 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA 4981 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG 5041 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 5101 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA 5161 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 5221 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG 5281 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA 5341 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTT 5401 GCATGCCTGC AGGTCGACTC TAGACCTATA CAGTTGAAGT CGGAAGTTTA CATACACTTA 5461 AGTTGGAGTC ATTAAAACTC GTTTTTCAAC TACTCCACAA ATTTCTTGTT AACAAACAAT 5521 AGTTTTGGCA AGTCAGTTAG GACATCTACT TTGTGCATGA CACAAGTCAT TTTTCCAACA 5581 ATTGTTTACA GACAGATTAT TTCACTTATA ATTCACTGTA TCACAATTCC AGTGGGTCAG 5641 AAGTTTACAT ACACTAAGTT GACTGTGCCT TTAAACAGCT TGGAAAATTC CAGAAAATGA 5701 TGTCATGGCT TTAGAAGCTT CTGATAGACT AATTGACATC ATTTGAGTCA ATTGGAGGTG 5761 TACCTGTGGA TGTATTTCAA GGGGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC 5821 AAACCACAAC TAGAATGCAG CGGCCGCTAG CTCGAGATCT TAATTAAACT AGTATCGATA 5881 TAACTTCGTA TAATGTATGC TATACGAAGT TATATGTACG GGCCAGATAT ACGCGTTGAC 5941 ATTGATTATT GACTAGGCTT TTGCAAAAAG CTTTGCAAAG ATGGATAAAG TTTTAAACAG 6001 AGAGGAATCT TTGCAGCTAA TGGACCTTCT AGGTCTTGAA AGGAGTGGGA ATT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.