pAAV-U6-Reg4-shRNA1-ZsGreen1 p

pAAV-U6-Reg4-shRNA1-ZsGreen1 plasmid

PVT99833

pAAV-U6-Reg4-shRNA1-ZsGreen1 plasmid Information

Plasmid name: pAAV-U6-Reg4(mouse)-shRNA1-ZsGreen1
Alias: GAGCTGGAGTGTCAGTCATAT
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pAAV-U6-Reg4-shRNA1-ZsGreen1 plasmid Description

pAAV-U6-Reg4-shRNA1-ZsGreen1 is a plasmid suitable for interference silencing in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-U6-Reg4-shRNA1-ZsGreen1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg CGTTACATAA CTTACGGTAA 241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 301 TTCCCATAGT AACGTCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 601 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 661 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG 841 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT 901 AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA 961 CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC 1021 CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT 1081 AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT 1141 ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG 1201 TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT 1261 GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA 1321 TCGATTGAAT TCCACCATGG CCCAGTCCAA GCACGGCCTG ACCAAGGAGA TGACCATGAA 1381 GTACCGCATG GAGGGCTGCG TGGACGGCCA CAAGTTCGTG ATCACCGGCG AGGGCATCGG 1441 CTACCCCTTC AAGGGCAAGC AGGCCATCAA CCTGTGCGTG GTGGAGGGCG GCCCCTTGCC 1501 CTTCGCCGAG GACATCTTGT CCGCCGCCTT CATGTACGGC AACCGCGTGT TCACCGAGTA 1561 CCCCCAGGAC ATCGTCGACT ACTTCAAGAA CTCCTGCCCC GCCGGCTACA CCTGGGACCG 1621 CTCCTTCCTG TTCGAGGACG GCGCCGTGTG CATCTGCAAC GCCGACATCA CCGTGAGCGT 1681 GGAGGAGAAC TGCATGTACC ACGAGTCCAA GTTCTACGGC GTGAACTTCC CCGCCGACGG 1741 CCCCGTGATG AAGAAGATGA CCGACAACTG GGAGCCCTCC TGCGAGAAGA TCATCCCCGT 1801 GCCCAAGCAG GGCATCTTGA AGGGCGACGT GAGCATGTAC CTGCTGCTGA AGGACGGTGG 1861 CCGCTTGCGC TGCCAGTTCG ACACCGTGTA CAAGGCCAAG TCCGTGCCCC GCAAGATGCC 1921 CGACTGGCAC TTCATCCAGC ACAAGCTGAC CCGCGAGGAC CGCAGCGACG CCAAGAACCA 1981 GAAGTGGCAC CTGACCGAGC ACGCCATCGC CTCCGGCTCC GCCTTGCCCT AAAGATCTAC 2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 2521 attttgtagg taaccacgtg cggaccTCGA AATTCCCCAG TGGAAAGACG CGCAGGCAAA 2581 ACGCACCACG TGACGGAGCG TGACCGCGCG CCGAGCGCGC GCCAAGGTCG GGCAGGAAGA 2641 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT 2701 AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA 2761 GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT 2821 GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGGTTTA TATATCTTGT GGAAAGGACG 2881 CGGGATCCgG AGCTGGAGTG TCAGTCATAT ttcaagagaA TATGACTGAC ACTCCAGCTC 2941 TTTTTTctcg agaccttagg caAAGCTTtg cgagttgcac acgtgtcAAG GCTGCCAGCT 3001 aggttaatct caattcttgg aagttagAGC TCGCTTTTTA AGCTTGCGGc cgcaggaacc 3061 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 3121 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 3181 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 3241 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg 3301 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 3361 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 3421 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 3481 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 3541 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 3601 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 3661 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 3721 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga 3781 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 3841 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 3901 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 3961 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 4021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 4081 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 4141 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 4201 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 4261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 4321 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 4381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 4441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 4501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 4561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 4621 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 4681 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 4741 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 4801 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 4861 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 4921 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 4981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 5041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 5101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 5161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 5221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 5281 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 5341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 5401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 5461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 5521 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 5581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 5641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 5701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 5761 ctggcctttt gctggccttt tgctcacatg t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.