pLV3-CMV-TUBA1B-EGFP-EF1a-Puro

pLV3-CMV-TUBA1B-EGFP-EF1a-Puro plasmid

PVT99718

pLV3-CMV-TUBA1B-EGFP-EF1a-Puro plasmid Information

Plasmid name: pLV3-CMV-TUBA1B(human)-EGFP-EF1a-Puro
Alias: NM_006082.3;K-ALPHA-1
Gene lenght: 1353bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TUBA1B-EGFP-EF1a-Puro plasmid Description

pLV3-CMV-TUBA1B-EGFP-EF1a-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TUBA1B-EGFP-EF1a-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgc gtgagtgcat ctccatccac gttggccagg ctggtgtcca 2341 gattggcaat gcctgctggg agctctactg cctggaacac ggcatccagc ccgatggcca 2401 gatgccaagt gacaagacca ttgggggagg agatgactcc ttcaacacct tcttcagtga 2461 gacgggcgct ggcaagcacg tgccccgggc tgtgtttgta gacttggaac ccacagtcat 2521 tgatgaagtt cgcactggca cctaccgcca gctcttccac cctgagcagc tcatcacagg 2581 caaggaagat gctgccaata actatgcccg agggcactac accattggca aggagatcat 2641 tgaccttgtg ttggaccgaa ttcgcaagct ggctgaccag tgcaccggtc ttcagggctt 2701 cttggttttc cacagctttg gtgggggaac tggttctggg ttcacctccc tgctcatgga 2761 acgtctctca gttgattatg gcaagaagtc caagctggag ttctccattt acccagcacc 2821 ccaggtttcc acagctgtag ttgagcccta caactccatc ctcaccaccc acaccaccct 2881 ggagcactct gattgtgcct tcatggtaga caatgaggcc atctatgaca tctgtcgtag 2941 aaacctcgat atcgagcgcc caacctacac taaccttaac cgccttatta gccagattgt 3001 gtcctccatc actgcttccc tgagatttga tggagccctg aatgttgacc tgacagaatt 3061 ccagaccaac ctggtgccct acccccgcat ccacttccct ctggccacat atgcccctgt 3121 catctctgct gagaaagcct accatgaaca gctttctgta gcagagatca ccaatgcttg 3181 ctttgagcca gccaaccaga tggtgaaatg tgaccctcgc catggtaaat acatggcttg 3241 ctgcctgttg taccgtggtg acgtggttcc caaagatgtc aatgctgcca ttgccaccat 3301 caaaaccaag cgcagcatcc agtttgtgga ttggtgcccc actggcttca aggttggcat 3361 caactaccag cctcccactg tggtgcctgg tggagacctg gccaaggtac agagagctgt 3421 gtgcatgctg agcaacacca cagccattgc tgaggcctgg gctcgcctgg accacaagtt 3481 tgacctgatg tatgccaagc gtgcctttgt tcactggtac gtgggtgagg ggatggagga 3541 aggcgagttt tcagaggccc gtgaagatat ggctgccctt gagaaggatt atgaggaggt 3601 tggtgtggat tctgttgaag gagagggtga ggaagaagga gaggaatacg gatccGGAGG 3661 AGGGGGAAGC atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 3721 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 3781 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 3841 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 3901 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 3961 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 4021 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 4081 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 4141 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 4201 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 4261 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 4321 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 4381 gtacaagtaa gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc 4441 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 4501 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 4561 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 4621 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg 4681 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg 4741 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac 4801 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt 4861 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca 4921 gatccaagct gtgaccggcg cctacgctag atgaccgagt acaagcccac ggtgcgcctc 4981 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac 5041 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa 5101 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5161 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5221 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5281 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5341 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5401 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5461 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5521 tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga ttacaaaatt 5581 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5641 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5701 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5761 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5821 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 5881 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 5941 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 6001 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6061 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 6121 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 6181 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6241 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6301 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6361 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6421 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6481 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6541 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6601 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6661 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6781 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca 6841 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 6901 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 6961 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 7021 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 7081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8281 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8581 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 9001 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 9061 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 9121 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 9181 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 9241 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 9301 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 9361 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 9421 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.