pLV3-CMV-Apod-3×FLAG-CopGFP-Pu

pLV3-CMV-Apod-3×FLAG-CopGFP-Puro plasmid

PVT99629

pLV3-CMV-Apod-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Apod(rat)-3×FLAG-CopGFP-Puro
Alias: NM_012777.2;AOPDGN
Gene lenght: 567bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Apod-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Apod-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Apod-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgaccatgct gttgctcctg 2341 gccacactgg caggtctctt caccacaacc gaaggacaaa gcttccatct tgggaaatgc 2401 ccatctcctc cggtgcaaga gaatttcgat gtgaaaaagt atcttggaag atggtacgaa 2461 atcgagaaga tcccggtgag ctttgagaaa ggaaactgca ttcaagccaa ctactcgctg 2521 atggagaacg gaaacatcaa agtgctaaac aaggagctgc gtcctgacgg aaccctgaac 2581 caagttgagg gtgaagccaa acagagcaac atgtcagagc cagccaagct ggaagtccag 2641 ttcttctcgt tgatgccacc ggcaccctac tggatcctgg ccacggatta cgagagctat 2701 gccctcgtgt attcctgcac caccttcttc tggttcttcc acgtggacta tgtttggatc 2761 ctgggaagaa atccttacct ccctccagaa acaataacct acctaaaata tatccttact 2821 tctaatgaca tcgacatcgc caaaataaca acaacagacc aagcaaactg cccggacttc 2881 ctgggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 2941 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 3001 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3061 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3121 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3181 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 3241 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 3301 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 3361 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 3421 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 3481 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 3541 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 3601 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 3661 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 3721 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 3781 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 3841 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 3901 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 3961 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 4021 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4081 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 4141 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 4201 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 4261 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 4321 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4381 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4441 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4501 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4561 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 4621 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4681 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4741 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 4801 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4861 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 4921 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 4981 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 5041 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 5101 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 5161 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 5221 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 5281 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 5341 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 5401 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 5461 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 5521 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 5581 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 5641 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 5701 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 5761 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 5821 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 5881 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 5941 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6001 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 6061 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6121 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6181 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 6241 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6301 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6421 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6601 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7141 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7201 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7261 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7321 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7381 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7441 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7501 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7561 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7621 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 7681 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 7741 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 7801 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 7861 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 7921 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 7981 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8041 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8101 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8161 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8221 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8281 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8341 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 8401 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 8461 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 8521 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 8581 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 8641 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 8701 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 8761 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 8821 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.