pCMV-Lars2-3×FLAG-Neo plasmid

pCMV-Lars2-3×FLAG-Neo plasmid

PVT108879

pCMV-Lars2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Lars2(mouse)-3×FLAG-Neo
Alias: NM_153168.4;Kiaa0028; LEURS
Gene lenght: 2706
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Lars2-3×FLAG-Neo plasmid Description

pCMV-Lars2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Lars2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatggc atccacttgt cagaggctga 781 gtttctatgt ctctcctctg aaaaggcagc tagttagcag gccacctgtc atcctgtggg 841 aaagactaat tccagggtgt tccagaagta tctacagtgc caccgggaag tggacaaagg 901 agtacacact gcagacaagg aaggatgtgg agaagtggtg gcaccaacag ataaaggagc 961 aagcgtccag agtctcagag gaagataaat taaagcccaa attctacttg ctctccatgt 1021 tcccataccc ttctggcaag ctgcacatgg gccatgtccg tgtctacacc ctcagcgaca 1081 ccattgcacg gttccagaaa atgagaggca tgcaggttat caaccccatg gggtgggatg 1141 catttggatt gcctgctgaa aatgctgcca tagagaggaa tttgcaccct gaaagctgga 1201 cccaaagcaa tattaaacac atgaggaagc agctcgaccg cctcggcctg tgcttcagct 1261 gggacaggga aatcactaca tgtttgccag attactacaa atggactcaa tacctgttta 1321 tcaaactcta tgaagcagga ctggcctatc aaaaggaggc cttggttaac tgggatccag 1381 tggatcaaac agtgctagcc aatgagcagg tgaatgaata tggctgttca tggcgttctg 1441 gagccaaggt agagaagaaa tacctccgac agtggttcat taagacaact gcttatgcaa 1501 aggccatgca ggatgcgttg gcagacctcc cagagtggta cggaatcaaa ggcatgcagg 1561 cccactggat tggggactgt gtaggctgtc acctggactt cacattaaag gttgatggag 1621 aagacacagg agagaaattg acagcataca cagccacccc tgaggccatc tatggcatct 1681 cccacgtggc catctctcct agccacgggc ttctacatgg ctgcagctct gtgaagaaag 1741 ccttgcagaa ggcactagtc cctggcagag attgcctcac accagtgatg gctgtgagca 1801 tgctcaccct gcaggaggtc cccatcgtta tcatggctaa ccctgacttg gaaggctctc 1861 tagattcaaa aataggaatc cccagcacca gctcagagga caccagactg gcccaagctc 1921 tgggcctacc ctactctgag gtcattgaag cttcacccga tggcacagag agactgagtg 1981 gatctgctga gtttacaggt atgaccaggc aggatgcctt tgtagccctg actcggaaag 2041 cccgtgggat gagagtgggt ggacacgtga caagtaacaa gctgaaggac tggctgatct 2101 cacggcagcg ctactggggc acaccgatcc ccattgtcca ctgcccagcc tgtggccctg 2161 tgcctgtgcc actgcaagac ttgcctgtga ttttgcccag cattgcatct ttaacgggca 2221 gaggaggctc cccgctggcc acggctttgg agtgggtgaa ctgctcctgc ccaaggtgca 2281 agggatcagc aaagagagag acggacacca tggacacctt tgtggattct gcgtggtact 2341 acttcagata cactgaccct cataatactc aaagtccttt tggctcggca ctggcagact 2401 tctggatgcc cgtggatctg tacatcggag gaaaggaaca tgctgtcatg cacttgttct 2461 atgcacgatt cctcagtcat ttttgccacg accaaaagat ggtgaagcac agggaacctt 2521 tccataaact actggcccaa ggcctcatta agggacagac attccgcctt ccatctggac 2581 agtgtctgaa gaaggaggat atagatttca caggtcctgc tcctgtttgt gcaaaaacaa 2641 aagagaagtt agaggtgacg tgggagaaga tgagcaagtc caaacacaat ggggtggacc 2701 ctgaggagat tgtggcccaa tatggaattg acacaatccg gctctacatc ctctttgcag 2761 cccctcctga gaaggacatc ttgtgggatg taaagactga cgcactccct ggagtgctaa 2821 gatggcaaca gcggttgtgg tccctgacaa caagattcat cgaggcaagg acttccggga 2881 cagtccctca gcctcagctg ctgaacagca aggagaaaac caaggcccaa aacctctggg 2941 agtacaaaaa tgcagtcata gctcaggtga ccactcattt cacagaggac tttgccctga 3001 attctgtagt ttctcagctg atgggactca gcagtgccct ctcgcaagcc tctcagagag 3061 tggttctcca cagccctgag tttgaagatg ctctgtgtgc cctgctggtg atggctgctc 3121 ccctggcccc tcatgtaacc tcagaacttt gggcaggcct gacgctggta ccaagcaagc 3181 tctgtgacca ttatgcctgg gattctggtg tgatgcttca ggcctggccc actgtggact 3241 cacagttcct tcagaaacct gacatggtgc agatggctgt tctgatcaac aacaaggcct 3301 gtggcaagat ccctgtgccc cagcatgttg cccaggacca ggacaaagtc catgaacttg 3361 ttctccagag cgagctaggc atgaagctgc tgcaggggag aagcatcaag aaggccttcc 3421 tctcccccag gactgccctc atcaacttct tggtgcagga gGGATCCGAC TACAAAGACC 3481 ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG 3541 GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 3601 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 3661 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 3721 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 3781 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 3841 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 3901 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC 3961 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 4021 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 4081 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 4141 CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC 4201 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA 4261 TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG 4321 GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG 4381 CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG 4441 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 4501 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 4561 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT 4621 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 4681 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 4741 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 4801 GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA 4861 GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 4921 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 4981 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5041 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 5101 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 5161 GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 5221 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 5281 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 5341 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 5401 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 5461 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 5521 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 5581 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 5641 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 5701 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 5761 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 5821 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 5881 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 5941 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 6001 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 6061 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC 6121 CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA 6181 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA 6241 TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 6301 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 6361 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA 6421 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 6481 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 6541 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 6601 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 6661 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 6721 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 6781 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 6841 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 6901 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 6961 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 7021 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 7081 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 7141 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 7201 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 7261 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 7321 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 7381 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 7441 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 7501 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 7561 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 7621 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 7681 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 7741 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 7801 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 7861 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 7921 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 7981 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 8041 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 8101 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 8161 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 8221 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 8281 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 8341 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 8401 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 8461 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 8521 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 8581 AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT 8641 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG 8701 GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT 8761 TGCATGAAGA ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.