pUC57-hRSV-P-BFP(HRSV) plasmid

pUC57-hRSV-P-BFP(HRSV) plasmid

PVT99452

pUC57-hRSV-P-BFP(HRSV) plasmid Information

Plasmid name: pUC57-hRSV-P-BFP(HRSV)
Alias: -
Gene lenght: 2803bp
Host: E.coli
Use(s): Gene Template
Fragment type: cDNA
Species: Human syncytial virus
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Blue
Promoter: T7
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: lac-P
Reverse primer:
Copy number: High
Induction:

pUC57-hRSV-P-BFP(HRSV) plasmid Description

pUC57-hRSV-P-BFP(HRSV) is a plasmid suitable for gene templates in Escherichia coli.

pUC57-hRSV-P-BFP(HRSV) plasmid Sequence

1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 1921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 1981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 2041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 2101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 2161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 2221 ACCATGATTA CGCCAAGCTT GCATGCAGGC CTCTGCAGTC GACGGGCCCG GGATCCGTGA 2281 CACTATAGAA GCGGCCGCCT CCCTTAGCCA TCCGAGTGGA CGACGTCCTC CTTCGGATGC 2341 CCAGGTCGGA CCGCGAGGAG GTGGAGATGC CATGCCGACC CACGAGAAAA AAAGTGTCAA 2401 AAACTAATAT CTCGTAATTT AGTTAATACA CATATAAACC AATTAGATTA GGGTTTAAAT 2461 TTATTCCTCC AAGATTAAAA TGATAACTTT AGGATTAGTT CACTAAAAGT TATTTAAAAA 2521 ATTATATGAT TTTTAATTTT TAATAACTAT AATTCGAATA CAGTGTTAGT GTATAGCTAT 2581 AGGAATTTTT ATTATAAGAT CTTTATTCAT TATTCATTAT GAAAGTTGTA TAACAGACTA 2641 CCTGTGATTT TAATCAGTTT TTTAAGTTCA TTAGTTGTCA AGCTGTTTAA CAATTCACTT 2701 AGGTAAGGAT ATGTAGATTC TACCATATAT AAATGGTTAT AGTTTAGTTC TGTTGATCTG 2761 AAATTTAAAA CATGATTGAA CCACTTTAAG ATGTTCATAT GCTTATGATT TATAAGTTTA 2821 TTGCTGAAAA CTTCATTCCG TCCAGCTATA GAATATGATA GTATATCTCC ACTAACAACA 2881 CTCTTTAGTT TTGACAATGC AGTATTAATT CCTTTTTTTG TTATAGGGTA ACAAAGAAAG 2941 GGTATCAAAC TTTTAATATT TGCATCAATA GACTCTTTAT CAGCTTTCTT AGGCATGATG 3001 AAATTTTTGG TTCTTGATAG TATCAATTTA GCATTTTGTA CTACATTAAA TACTGGAAAT 3061 ATATTTGCAG GACCTATTGT AAGGACTAAG TAAACCTCCG ATCCCTTTAA CTTACTGCCT 3121 AAGCATACAT AAGTTTTTAA TATAGTTATA TTGTCTAATT TGAAATCAAT ATCATCTTGA 3181 GCATGATATT TTACTATTAA CGTACATTTA TTAACTGAGG AACAGTACTT GCATTTTCTT 3241 ACATGCTTGC TCCATTCTAT TATAATTTTA CTCCAGTTGA CTGTTACAGG CAATTCGGCA 3301 TCACATACAA AAAGACTGAT AGGTTCAGCA AACTTTATAT GTAAATAAGA CCAATGAATG 3361 TTGTTGGTTG CATCTGTAGC AGGAATGGTC AAATTTTCAC CATAATCAAT GTTGATATGT 3421 CCATTGTATA GCCTTAAAAA CTCAATAGGT AAACTATGAT CATTGCAATC TTTCAGACTT 3481 CTGTAAATAT ATCTTATGTC AGGATGAAGT TCCACCACTG TACGCAATAA TAAATTCCCT 3541 GCTCCTTCAC CTATGAATGC TATACAATTA GGATCTTTAA TTTTAAGGTC TTTTAAAATA 3601 TACTCTATAC TAATTTTACA ACCTGTAGAA CTAAATACAA AATTGAATCT ATTAATATGA 3661 TGCCAAGGAA GCATGCAATA AAGTGATGTG CTATTGTGCA CTAAAGATAT TTGATGGGAA 3721 GTAGTAGTGT AAAGTTGGTT GGATTTGGCT GTATTACCTG AATGATCTAT AATTCTATCG 3781 ATCACAACCG TAGGGAATAG ATTGTACAGG TCTTGTTTGC TGTAATTGGT TCTAATCATT 3841 GCAGACGATT TAACAAGCTT CTTATTAGAT AACAATGGTA ACATTATTGA GTCGACATTT 3901 TTATATGGTA CCTTAAGTTT TAAATTACTA TAATTTTCAG GCTCCATTTG GACTATGGAG 3961 TGTAGTTATG CATAAAGTTG TTGTTTTGGA TTGTGTGAAT CTTGTGTTGA AATTCATGGA 4021 TTGAGATCAT ACTTGTATAT TATGGGAGTA TGCTTTGTAG GCTTAATGCC AATGCATTCT 4081 AAGAACCCAT CATGATTGAT GAATATCGGC ATAGGGAAAG TGCCATATTT TGTGTTGTAT 4141 TCAGTATATT TTTTATATTT AGTGCTTCCT ACTTTGTGCA ATAGTTTCAT TTCATAGTTG 4201 ACCAGGAATG TAAATGTGGC CTGTCTTTCA TCAAGTTTTC TCACTATGCA TTCATGATTT 4261 ATTAAGTATA TAAATCTGTG TGTTATGATG TCTCTGGTTA GTGATGTTAT TGTAGTCTCA 4321 AGTGACAACG GTCTCATGTC TGTGATCATC AGTCTTTGTG GTGTGGTATC ATTGTGGGTT 4381 GTGTCCATGG TTGGGTTGGC TGAGTTGATT TATTTGCCCC ATTTTTGTCT TCTGTTAAGT 4441 TTTATATTAA CTAATGGTGC TAGTGACATT GATTTGCTAG TTGATATTAA TTATAATTTA 4501 TGGATTAAGA TCAAATCCAA GTAATTCAGA TAATTGATTC ATATAATTGG TCATTGTTGA 4561 ATCACTTAGT TTTTTGGAGA ATTTAATTTC ACAATTGTCA TCTATTAGAC CATTAGGTTG 4621 AGAGCAATGT GTTAATTCCA TCATTTCCCA TATATAACCT CCATTTTGTA GCACTGGCAT 4681 TGTTGTGAAA TTGGATTTTA CTACAATATT ATTATTAGGG CAAATATCAC TACTTGTAAT 4741 AACATGCACA AACACAATGC CATTCAATTT GATTGTATGT ATCACTGCCT TAGCCAAAGC 4801 ATTAGTTAAA TGTATTAATT TGTCAGTATA GCATGTTATT TTTAACAATG CTACTTCATC 4861 ATTGTCAAAC AAATTTTGTA ATCTAACTTT TATCATACTC AACGAATTGC TGCCCATCTC 4921 TAACCAAGGG AGTTAAATTT AAGTGGTACT TATCAAATTC TTATTTGCCC CATTTTTTTG 4981 GTTTATGCAA GTTTGTTGTA CGCATTTTTT CGCGTGACGG AGTCTAGACT CCGTTTCGTC 5041 CTCACGGACT CATCAGACGC GTCTCCCTAT AGTGAGTCGG AATTCACTGG CCGTCGTTTT 5101 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC 5161 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT 5221 GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG 5281 TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG 5341 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC 5401 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC 5461 GTCATCACCG AAACGCGCGA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.