pLV3-CMV-SUMO1-3×Myc-EF1a-CopG

pLV3-CMV-SUMO1-3×Myc-EF1a-CopGFP-Puro plasmid

PVT99434

pLV3-CMV-SUMO1-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SUMO1(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_003352.8;DAP1; GMP1; OFC10; PIC1; SENP2; SMT3;SMT3C; SMT3H3; UBL1
Gene lenght: 303bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SUMO1-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-SUMO1-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SUMO1-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgt ctgaccagga ggcaaaacct tcaactgagg acttggggga 2341 taagaaggaa ggtgaatata ttaaactcaa agtcattgga caggatagca gtgagattca 2401 cttcaaagtg aaaatgacaa cacatctcaa gaaactcaaa gaatcatact gtcaaagaca 2461 gggtgttcca atgaattcac tcaggtttct ctttgagggt cagagaattg ctgataatca 2521 tactccaaaa gaactgggaa tggaggaaga agatgtgatt gaagtttatc aggaacaaac 2581 ggggggtcat tcaacagttg gatccGAACA AAAACTAATA TCAGAGGAAG ATTTGGAACA 2641 GAAACTAATC AGTGAAGAAG ATTTAGAGCA AAAGTTAATT TCCGAGGAGG ACTTATAgcg 2701 gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 2761 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 2821 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 2881 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 2941 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 3001 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 3061 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 3121 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 3181 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 3241 accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga 3301 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 3361 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 3421 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 3481 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 3541 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 3601 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 3661 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 3721 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 3781 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 3841 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 3901 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 3961 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 4021 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac 4081 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac 4141 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca 4201 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 4261 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 4321 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 4381 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 4441 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 4501 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 4561 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 4621 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca 4681 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 4741 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 4801 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 4861 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 4921 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 4981 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5041 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 5101 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5161 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct 5221 tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc 5281 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 5341 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg 5401 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 5461 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 5521 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 5581 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 5641 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 5761 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg 5821 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 5881 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca 5941 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 6001 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 6061 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 6121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 6181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 6421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 6481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 6541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 6601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 6661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 6721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 6781 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 6841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 6901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 7021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 7081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 7141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 7201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 7261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 7321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 7381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 7441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 7501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 7561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 7621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 7681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 7741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 7801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 7861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 7921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 7981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 8041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8101 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 8161 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 8221 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 8281 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 8341 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 8401 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 8461 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 8521 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.