pCMV-SMARCC2-3×Myc-Neo plasmid

pCMV-SMARCC2-3×Myc-Neo plasmid

PVT99262

pCMV-SMARCC2-3×Myc-Neo plasmid Information

Plasmid name: pCMV-SMARCC2(human)-3×Myc-Neo
Alias: NM_001330288.2;BAF170; CRACC2; CSS8; Rsc8
Gene lenght: 3735bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SMARCC2-3×Myc-Neo plasmid Description

pCMV-SMARCC2-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SMARCC2-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc ggtgcggaag 721 aaggacggcg gccccaacgt gaagtactac gaggccgcgg acaccgtgac ccagttcgac 781 aacgtgcggc tgtggctcgg caagaactac aagaagtata tacaagctga accacccacc 841 aacaagtccc tgtctagcct ggttgtacag ttgctacaat ttcaggaaga agtttttggc 901 aaacatgtca gcaatgcacc gctcactaaa ctgccgatca aatgtttcct agatttcaaa 961 gcgggaggct ccttgtgcca cattcttgca gctgcctaca aattcaagag tgaccaggga 1021 tggcggcgtt acgatttcca gaatccatca cgcatggacc gcaatgtgga aatgtttatg 1081 accattgaga agtccttggt gcagaataat tgcctgtctc gacctaacat ttttctgtgc 1141 ccagaaattg agcccaaact actagggaaa ttaaaggaca ttatcaagag acaccaggga 1201 acagtcactg aggataagaa caatgcctcc catgttgtgt atcctgtccc ggggaatcta 1261 gaagaagagg aatgggtacg accagtcatg aagagggata agcaggttct tctgcactgg 1321 ggctactatc ctgacagtta cgacacgtgg atcccagcga gtgaaattga ggcatctgtg 1381 gaagatgctc caactcctga gaaacctagg aaggttcatg caaagtggat cctggacacc 1441 gacaccttca atgaatggat gaatgaggaa gactatgaag taaatgatga caaaaaccct 1501 gtctcccgcc gaaagaagat ttcagccaag acactgacag atgaggtgaa cagcccagat 1561 tcagatcgac gggacaagaa ggggggaaac tataagaaga ggaagcgctc cccctctcct 1621 tcaccaaccc cagaagcaaa gaagaaaaat gctaagaaag gtccctcaac accttacact 1681 aagtcaaagc gtggccacag agaagaggag caagaagacc tgacaaagga catggacgag 1741 ccctcaccag tccccaatgt agaagaggtg acacttccca aaacagtcaa cacaaagaaa 1801 gactcagagt cggccccagt caaaggcggc accatgaccg acctggatga acaggaagat 1861 gaaagcatgg agacgacggg caaggatgag gatgagaaca gtacggggaa caagggagag 1921 cagaccaaga atccagacct gcatgaggac aatgtgactg aacagaccca ccacatcatc 1981 attcccagct acgctgcctg gtttgactac aatagtgttc atgccattga gcggagggct 2041 ctccccgagt tcttcaacgg caagaacaag tccaagactc cagagatcta cctggcctat 2101 cgaaacttta tgattgacac ttaccgactg aacccccaag agtatcttac ctctaccgcc 2161 tgccgccgaa acctagcggg tgatgtctgt gccatcatga gggtccatgc cttcctagaa 2221 cagtggggtc ttattaacta ccaggtggat gctgagagtc gaccaacccc aatggggcct 2281 ccgcctacct ctcacttcca tgtcttggct gacacaccat cagggctggt gcctctgcag 2341 cccaagacac ctcagggccg ccaggttgat gctgatacca aggctgggcg aaagggcaaa 2401 gagctggatg acctggtgcc agagacggct aagggcaagc cagagctgca gacctctgct 2461 tcccaacaaa tgctcaactt tcctgacaaa ggcaaagaga aaccaacaga catgcaaaac 2521 tttgggctgc gcacagacat gtacacaaaa aagaatgttc cctccaagag caaggctgca 2581 gccagtgcca ctcgtgagtg gacagaacag gaaaccctgc ttctcctgga ggcactggaa 2641 atgtacaaag atgactggaa caaagtgtcc gagcatgtgg gaagccgcac acaggacgag 2701 tgcatcttgc attttcttcg tcttcccatt gaagacccat acctggagga ctcagaggcc 2761 tccctaggcc ccctggccta ccaacccatc cccttcagtc agtcgggcaa ccctgttatg 2821 agcactgttg ccttcctggc ctctgtcgtc gatccccgag tcgcctctgc tgctgcaaag 2881 tcagccctag aggagttctc caaaatgaag gaagaggtac ccacggcctt ggtggaggcc 2941 catgttcgaa aagtggaaga agcagccaaa gtaacaggca aggcggaccc tgccttcggt 3001 ctggaaagca gtggcattgc aggaaccacc tctgatgagc ctgagcggat tgaggagagc 3061 gggaatgacg aggctcgggt ggaaggccag gccacagatg agaagaagga gcccaaggaa 3121 ccccgagaag gagggggtgc tatagaggag gaagcaaaag agaaaaccag cgaggctccc 3181 aagaaggatg aggagaaagg gaaagaaggc gacagtgaga aggagtccga gaagagtgat 3241 ggagacccaa tagtcgatcc tgagaaggag aaggagccaa aggaagggca ggaggaagtg 3301 ctgaaggaag tggtggagtc tgagggggaa aggaagacaa aggtggagcg ggacattggc 3361 gagggcaacc tctccaccgc tgctgccgcc gccctggccg ccgccgcagt gaaagctaag 3421 cacttggctg ctgttgagga aaggaagatc aaatctttgg tggccctgct ggtggagacc 3481 cagatgaaaa agttggagat caaacttcgg cactttgagg agctggagac tatcatggac 3541 cgggagcgag aagcactgga gtatcagagg cagcagctcc tggccgacag acaagccttc 3601 cacatggagc agctgaagta tgcggagatg agggctcggc agcagcactt ccaacagatg 3661 caccaacagc agcagcagcc accaccagcc ctgcccccag gctcccagcc tatcccccca 3721 acaggggctg ctgggccacc cgcagtccat ggcttggctg tggctccagc ctctgtagtc 3781 cctgctcctg ctggcagtgg ggcccctcca ggaagtttgg gcccttctga acagattggg 3841 caggcagggt caactgcagg gccacagcag cagcaaccag ctggagcccc ccagcctggg 3901 gcagtcccac caggggttcc cccccctgga ccccatggcc cctcaccgtt ccccaaccaa 3961 caaactcctc cctcaatgat gccaggggca gtgccaggca gcgggcaccc aggcgtggcg 4021 ggtaatgctc ctttgggttt gccttttggc atgccgcctc ctcctcctcc tcctgctcca 4081 tccatcatcc catttggtag tctagctgac tccatcagta ttaacctccc cgctcctcct 4141 aacctgcatg ggcatcacca ccatctcccg ttcgccccgg gcactctccc cccacctaac 4201 ctgcctgtgt ccatggcgaa ccctctacat cctaacctgc cggcgaccac caccatgcca 4261 tcttccttgc ctctcgggcc ggggctcgga tccgccgcag cccaaagccc tgccattgtg 4321 gcagctgttc agggcaacct cctgcccagt gccagcccac tgccagaccc aggcaccccc 4381 ctgcctccag accccacagc cccgagccca ggcacggtca cccctgtgcc acctccacag 4441 CTCGAGGAAC AAAAACTAAT ATCAGAGGAA GATTTGGAAC AGAAACTAAT CAGTGAAGAA 4501 GATTTAGAGC AAAAGTTAAT TTCCGAGGAG GACTTATAGT GAGGGCCCGA TATCTCGACA 4561 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 4621 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 4681 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 4741 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 4801 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 4861 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 4921 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 4981 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 5041 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 5101 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAAACGGGG 5161 GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT 5221 AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT 5281 CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG 5341 TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA 5401 ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC 5461 CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 5521 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 5581 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT 5641 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 5701 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 5761 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 5821 TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 5881 TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC 5941 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA 6001 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 6061 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 6121 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC 6181 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT 6241 CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG 6301 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT 6361 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC 6421 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT 6481 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT 6541 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA 6601 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT 6661 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG 6721 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA 6781 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA 6841 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA 6901 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA 6961 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT 7021 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT 7081 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA 7141 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 7201 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 7261 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 7321 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 7381 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 7441 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 7501 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 7561 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 7621 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 7681 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 7741 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 7801 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 7861 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 7921 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 7981 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT 8041 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 8101 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 8161 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 8221 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 8281 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 8341 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 8401 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 8461 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 8521 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 8581 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 8641 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 8701 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 8761 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 8821 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 8881 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 8941 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 9001 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 9061 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 9121 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 9181 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 9241 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 9301 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 9361 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 9421 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 9481 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 9541 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 9601 ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC 9661 CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC 9721 GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT 9781 TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.