pTRIM22-Fluc plasmid

pTRIM22-Fluc plasmid

PVT98891

pTRIM22-Fluc plasmid Information

Plasmid name: pTRIM22(human)-Fluc
Alias: -
Gene lenght: 1500bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: TRIM22
Replicon: SLC9A9
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pTRIM22-Fluc plasmid Description

pTRIM22-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pTRIM22-Fluc plasmid Sequence

1 ggtacctccc ttctttctca agatatagac actatgagtt gatgaaaata aaatttatga 61 taataagaac cacagtaata atagctatta ttgattagat atctactatt tttcagtctt 121 tgcatctact gctacttaca tatattatct catttagttc tcgccaaatt tcatgacaca 181 agttctactg gtatctgatt ttatggaaca gcaaagtttt gctaagttaa gtaatttccc 241 atcatttaca gctaataggt aggaaattca aaatttgaaa ctaggttgaa attcagatat 301 gtaacattaa tctggctcta tatttgcaat atttgctgtc aggtatagtg aaaggagaaa 361 gaaaagaaag aaaagtaagg tatgaggaaa aatacgagaa tttagaagag ccaaaacttc 421 ttcaaagaag aaaaaatctg caggatttac actatcagac actgagtctt cttataaagt 481 gatgataatt ttaacagtgt tttattagaa aaaaaacaca atacaacaga attgagtcaa 541 gaagcagatc cacacctttt ctagggtcta ggtatctaga aggaaggtat cccttagtgg 601 gaatttaaag taaattggta agggagaatg attatctgtg aaggttttcc accatatgaa 661 gttcttttac aagagcctat tgaccttgct aacagtttaa agaaatgtga catcaaatta 721 gattagcctg gagctctcta aacttcaaaa cttaagggta cagtcccgaa gactaccctc 781 acttctgtca ttaactgcaa tttcagaggt tttccaaaac caccctcagc ttcaataatt 841 cactagaagt acttatagaa ctcactgaaa gctattatgt tcatggttac agtttattac 901 aggaaaagga cacagattaa aattagacaa ataaagagat gcttagaaaa gaaactgagg 961 tgtccaaatc tgaagaattt gttgtccttt catggagtaa acatgcatta ctcttatggc 1021 attaatgtgt gacaatatgc atgaagtcat attgccaact agggaaattc tcccaagctt 1081 tggtgttcag agtttttatt gggatttcat tatataggta tgatggctgg attgattgca 1141 aatgtggttg aactccgtct ccaggtttac tggtgtcaca tgacccaaag tccccacact 1201 aagtcatatt gttgatcttt atgacatggc cagtccctac cctcagatct agtgtgacca 1261 atccccagtc taataaacaa agactctctt attaggtatt gtgtatATTT CCTTCTAGAA 1321 GCCAGGAGCA AAGGACAGAC CTCTCTCTGG gtaaagtcaa attttttagc acacatctgc 1381 ttatttagtt ccaaatatat tatttactac tgctatatgt gagcttttta cctcatgtct 1441 tatatgggcc ttgggctcag ttataaattc aggtttctta ataatcaaat ttgagttcag 1501 ttaaataagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca 1561 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc 1621 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata 1681 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta 1741 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc 1801 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg 1861 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt 1921 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc 1981 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt 2041 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg 2101 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag 2161 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt 2221 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt 2281 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata 2341 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc 2401 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat 2461 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg 2521 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat 2581 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc 2641 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa 2701 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag 2761 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg 2821 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct 2881 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg 2941 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt 3001 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa 3061 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta 3121 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa 3181 agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat 3241 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 3301 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 3361 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 3421 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg 3481 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca 3541 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc 3601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3901 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3961 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4021 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4081 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4141 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4201 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4261 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 4441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 4561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 4621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 4681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 4741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 4801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 4861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 4921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 4981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 5041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 5161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 5221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 5281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 5341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 5401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 5461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 5521 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 5581 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 5641 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 5701 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 5761 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 5821 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 5881 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 5941 atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc cattcaggct 6001 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct 6061 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata 6121 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac 6181 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca 6241 agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.