pCCL22-Fluc plasmid

pCCL22-Fluc plasmid

PVT95820

pCCL22-Fluc plasmid Information

Plasmid name: pCCL22(human)-Fluc
Alias:
Gene lenght: 2000bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: CCL22
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pCCL22-Fluc plasmid Description

pCCL22-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pCCL22-Fluc plasmid Sequence

1 ggtaccCAGG AGCCCGGGAC CTGCCTTTAG GTGAATGGGG AGCCACGGCA GGGTTCTGAA 61 GCAGGAGAGA GAGTGTGTAG CTCTGGACCA CTCACGCTGG CTGCTGCAGG GTGGGGATGG 121 CATTGAGGAG GCCAGCATCC CAGCCTGAGG TGGAGGTGGC CTGGACTGGG CAGGAACAGT 181 GGGGTTGGAG AGAAGGAGCA GATCTGAGAG AGATTTAGGA AGCAGAATCA ACGGGACATG 241 GACATGGTGA GGGACTGAGA CAGGAGTGGG GGAGGGAGAG CCCGGCAGCC ACTGGGAAGG 301 AAGATCAGGC AGGACAGAAG TGTGGGTAGA TGCTTTCCAT AAACCGTTGA GAAATCCACG 361 ACCTGGCCCA AATGTGGACT TCACGAGAAG CCCCAGATGG GTCAATGGGT CCATAGGCCT 421 CTCCCATTGA AGAGAGACCA GTAATGCCGT CATTAAGGGT ACAAGACAGG CCTCAATTCT 481 AGGTGAGGGT TGGTCCACGT TCTCACTTGA TTTACAACCA GTGAAGTCAA CAGAGCTGTG 541 AAACCATCTC CCCCATTTCC AGATGGGGAA ACCAAGTCCC AGAGACACCC AGACATGGCC 601 AAGGCCACAC AGTGCAGAGA CCCTGGGGAG CACAAGCCTC TAAGAGGGGG ATTTTACAAG 661 TCTGTCTGAA ACCAGAGGCG AGGACAGAGT TGAGGGGGGG TCCCCTCTGA GTGGTTCCCC 721 GCCAAAGAGA ATTTCTCCAC TTTGGAGGAA GTGGGAGGTA GTTCTTCTTT TGAGATGTGA 781 ATGTCAAGTG ACTCTGGGCA CCCCGGTGAC TAAGAGGTAA GTAAGTGAGG CTTGTGGGTG 841 GAGCCAGCAC CTTAAATAGC AGGTCTTCCT ATGTCCCTTT GCAGACACCT GGGCTGAGAC 901 ATACAGGACA GAGCATGGAT CGCCTACAGA CTGCACTCCT GGTTGTCCTC GTCCTCCTTG 961 CTGTGGCGCT TCAAGCAACT GAGGCAGGTG AGGCTGGGGA GCAGGAAGAC CCCCTACAGA 1021 GGCCAGGGCA GACGGTGGGG TGTCTTCCTC ATGTCTTGGA CAAGCACTGG ACCAAGAGCA 1081 GAAGACCTCA GTCTGCTGTT GGCTCTTGCG GCCTTCGGCT AGTTGCAGTG CTTCTCTGGG 1141 CCTCAGTTTA CAAGTCTGTT GAATGGGCAG TCTTGAAGAC TTGGAGTCTA GGATCTGTCT 1201 CACCTGGGTT GGGGGTTGTG GTGGGCATAG CCTCGGGATC TCCTGCCCCA GAAAACTCAA 1261 GCATAGGGCC CTAGGGGTGT GGGTGAGGAG CTTCTAAGGG TCAGGTCTGA GTGGGGGAAG 1321 GCAGGGCTGG GGCAAGGGGT TCTCCCTTGG AAAGCTGGTG CTGCCCTCCC ACCATCCATT 1381 TTTTAAAATG TTATTATTAT TATTTTTTAT TTTATTTTAT TTTATTTTTT GAGACACAGT 1441 TTCACTCTTG TTGCCCAGGA TGGAGTGCAG TGGGGCAATC TTGGCCCACT GCAACCTCTA 1501 CCTCCCAGGT TCAAGCGATT CTCCTGCCTC AGCCTCCTGA GTAGCTGGGA TTACAGGCAT 1561 GCACCACCAC ACCTGGCTAA TTTTTGTATT TTTAGTGGAG ACAGGGTTTC ACCATGTTGG 1621 CCAGGCTGGT CTCAAACTCC TGACCTCAGA TGATCCTCCC ACCTCAGCCT CCCAAAGTGC 1681 TGGGATTACA GGCATGAGCC ACTGCGTCTA GCCTAAATTT ATTTTTTATT TCATTTTATT 1741 TATTTATTTT TTGAGATGGA GTCTCTGTTG CCCAGGCTGG AGTGCAGTGG CGCAATCTCA 1801 GCTCACTGCA ACCTCCACCT CTGGAGTTAA AGTGATTCTC CTGCCTCAGC CTCCTGAGTA 1861 GCTGGGATTA CAGGCACCTG CCACCATGCC CGGCTAATTT TTGTATTTTT AGTAAAGATG 1921 GGGTTTCACC ATGCTGACCG GTCTTGTCTC GAACTCCTGA CCTCAAGTGA AACTCCCATC 1981 TTGGCCTCCC TAAGTGTTAG GATTACaagc ttggcattcc ggtactgttg gtaaagccac 2041 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 2101 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 2161 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 2221 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 2281 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 2341 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 2401 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 2461 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 2521 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 2581 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 2641 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 2701 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 2761 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 2821 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 2881 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 2941 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 3001 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 3061 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 3121 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 3181 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 3241 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 3301 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 3361 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 3421 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 3481 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 3541 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 3601 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 3661 aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct 3721 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 3781 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 3841 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 3901 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga 3961 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 4021 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 4081 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4441 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 4501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 4861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 4921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 4981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 5941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6001 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 6061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 6121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 6181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 6241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 6301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 6361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 6421 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 6481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 6541 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt 6601 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg 6661 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa 6721 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.