pLV3-CMV-Ighm-3×FLAG-CopGFP-Pu

pLV3-CMV-Ighm-3×FLAG-CopGFP-Puro plasmid

PVT98734

pLV3-CMV-Ighm-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Ighm(mouse)-3×FLAG-CopGFP-Puro
Alias: A0A075B5P6
Gene lenght: 1362bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ighm-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Ighm-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Ighm-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgA GTCAGTCCTT CCCAAATGTC 2341 TTCCCCCTCG TCTCCTGCGA GAGCCCCCTG TCTGATAAGA ATCTGGTGGC CATGGGCTGC 2401 CTGGCCCGGG ACTTCCTGCC CAGCACCATT TCCTTCACCT GGAACTACCA GAACAACACT 2461 GAAGTCATCC AGGGTATCAG AACCTTCCCA ACACTGAGGA CAGGGGGCAA GTACCTAGCC 2521 ACCTCGCAGG TGTTGCTGTC TCCCAAGAGC ATCCTTGAAG GTTCAGATGA ATACCTGGTA 2581 TGCAAAATCC ACTACGGAGG CAAAAACAAA GATCTGCATG TGCCCATTCC AGCTGTCGCA 2641 GAGATGAACC CCAATGTAAA TGTGTTCGTC CCACCACGGG ATGGCTTCTC TGGCCCTGCA 2701 CCACGCAAGT CTAAACTCAT CTGCGAGGCC ACGAACTTCA CTCCAAAACC GATCACAGTA 2761 TCCTGGCTAA AGGATGGGAA GCTCGTGGAA TCTGGCTTCA CCACAGATCC GGTGACCATC 2821 GAGAACAAAG GATCCACACC CCAAACCTAC AAGGTCATAA GCACACTTAC CATCTCTGAA 2881 ATCGACTGGC TGAACCTGAA TGTGTACACC TGCCGTGTGG ATCACAGGGG TCTCACCTTC 2941 TTGAAGAACG TGTCCTCCAC ATGTGCTGCC AGTCCCTCCA CAGACATCCT AACCTTCACC 3001 ATCCCCCCCT CCTTTGCCGA CATCTTCCTC AGCAAGTCCG CTAACCTGAC CTGTCTGGTC 3061 TCAAACCTGG CAACCTATGA AACCCTGAAT ATCTCCTGGG CTTCTCAAAG TGGTGAACCA 3121 CTGGAAACCA AAATTAAAAT CATGGAAAGC CATCCCAATG GCACCTTCAG TGCTAAGGGT 3181 GTGGCTAGTG TTTGTGTGGA AGACTGGAAT AACAGGAAGG AATTTGTGTG TACTGTGACT 3241 CACAGGGATC TGCCTTCACC ACAGAAGAAA TTCATCTCAA AACCCAATGA GGTGCACAAA 3301 CATCCACCTG CTGTGTACCT GCTGCCACCA GCTCGTGAGC AACTGAACCT GAGGGAGTCA 3361 GCCACAGTCA CCTGCCTGGT GAAGGGCTTC TCTCCTGCAG ACATCAGTGT GCAGTGGCTT 3421 CAGAGAGGGC AACTCTTGCC CCAAGAGAAG TATGTGACCA GTGCCCCGAT GCCAGAGCCT 3481 GGGGCCCCAG GCTTCTACTT TACCCACAGC ATCCTGACTG TGACAGAGGA GGAATGGAAC 3541 TCCGGAGAGA CCTATACCTG TGTTGTAGGC CACGAGGCCC TGCCACACCT GGTGACCGAG 3601 AGGACCGTGG ACAAGTCCAC TGGTAAACCC ACACTGTACA ATGTCTCCCT GATCATGTCT 3661 GACACAGGCG GCACCTGCTA TggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3721 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 3781 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3841 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3901 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3961 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4021 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4081 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4141 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4201 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4261 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4321 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4381 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 4441 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4501 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4561 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4621 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4681 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4741 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 4801 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 4861 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 4921 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 4981 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 5041 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 5101 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 5161 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5221 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5281 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5341 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5401 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5461 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5521 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5581 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5641 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5701 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 5761 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5821 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5881 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5941 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6001 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6061 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6121 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 6181 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6241 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 6301 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6361 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6421 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6481 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6541 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6601 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6661 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6721 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6781 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6841 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 6901 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 6961 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 7021 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7081 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7141 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7201 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7321 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7861 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7981 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 8041 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 8101 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 8161 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 8221 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8281 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8341 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8401 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8461 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8521 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8581 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8641 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8701 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8761 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8821 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 8881 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 8941 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 9001 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 9061 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 9121 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 9181 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 9241 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 9301 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9361 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9421 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9481 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9541 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9601 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.