pLV3-CMV-MECOM-3×FLAG-CopGFP-P

pLV3-CMV-MECOM-3×FLAG-CopGFP-Puro plasmid

PVT98664

pLV3-CMV-MECOM-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MECOM(human)-3×FLAG-CopGFP-Puro
Alias: NM_001105077.4;AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;PRDM3; RUSAT2
Gene lenght: 3348bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MECOM-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MECOM-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MECOM-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tcttagacga attttacaat 2341 gtgaagttct gcatagatgc cagtcaacca gatgttggaa gctggctcaa gtacattaga 2401 ttcgctggct gttatgatca gcacaacctt gttgcatgcc agataaatga tcagatattc 2461 tatagagtag ttgcagacat tgcgccggga gaggagcttc tgctgttcat gaagagcgaa 2521 gactatcccc atgaaactat ggcgccggat atccacgaag aacggcaata tcgctgcgaa 2581 gactgtgacc agctctttga atctaaggct gaactagcag atcaccaaaa gtttccatgc 2641 agtactcctc actcagcatt ttcaatggtt gaagaggact ttcagcaaaa actcgaaagc 2701 gagaatgatc tccaagagat acacacgatc caggagtgta aggaatgtga ccaagttttt 2761 cctgatttgc aaagcctgga gaaacacatg ctgtcacata ctgaagagag ggaatacaag 2821 tgtgatcagt gtcccaaggc atttaactgg aagtccaatt taattcgcca ccagatgtca 2881 catgacagtg gaaagcacta tgaatgtgaa aactgtgcca agcaggtttt cacggaccct 2941 agcaaccttc agcggcacat tcgctctcag catgtcggtg cccgggccca tgcatgcccg 3001 gagtgtggca aaacgtttgc cacttcgtcg ggcctcaaac aacacaagca catccacagc 3061 agtgtgaagc cctttatctg tgaggtctgc cataaatcct atactcagtt ttcaaacctt 3121 tgccgtcata agcgcatgca tgctgattgc agaacccaaa tcaagtgcaa agactgtgga 3181 caaatgttca gcactacgtc ttccttaaat aaacacagga ggttttgtga gggcaagaac 3241 cattttgcgg caggtggatt ttttggccaa ggcatttcac ttcctggaac cccagctatg 3301 gataaaacgt ccatggttaa tatgagtcat gccaacccgg gccttgctga ctattttggc 3361 gccaataggc atcctgctgg tcttaccttt ccaacagctc ctggattttc ttttagcttc 3421 cctggtctgt ttccttccgg cttgtaccac aggcctcctt tgatacctgc tagttctcct 3481 gttaaaggac tatcaagtac tgaacagaca aacaaaagtc aaagtcccct catgacacat 3541 cctcagatac tgccagctac acaggatatt ttgaaggcac tatctaaaca cccatctgta 3601 ggggacaata agccagtgga gctccagccc gagaggtcct ctgaagagag gccctttgag 3661 aaaatcagtg accagtcaga gagtagtgac cttgatgatg tcagtacacc aagtggcagt 3721 gacctggaaa caacctcggg ctctgatctg gaaagtgaca ttgaaagtga taaagagaaa 3781 tttaaagaaa atggtaaaat gttcaaagac aaagtaagcc ctcttcagaa tctggcttca 3841 ataaataata agaaagaata cagcaatcat tccattttct caccatcttt agaggagcag 3901 actgcggtgt caggagctgt gaatgattct ataaaggcta ttgcttctat tgctgaaaaa 3961 tactttggtt caacaggact ggtggggctg caagacaaaa aagttggagc tttaccttac 4021 ccttccatgt ttcccctccc attttttcca gcattctctc aatcaatgta cccatttcct 4081 gatagagact tgagatcgtt acctttgaaa atggaacccc aatcaccagg tgaagtaaag 4141 aaactgcaga agggcagctc tgagtccccc tttgatctca ccactaagcg aaaggatgag 4201 aagcccttga ctccagtccc ctccaagcct ccagtgacac ctgccacaag ccaagaccag 4261 cccctggatc taagtatggg cagtaggagt agagccagtg ggacaaagct gactgagcct 4321 cgaaaaaacc acgtgtttgg gggaaaaaaa ggaagcaacg tcgaatcaag acctgcttca 4381 gatggttcct tgcagcatgc aagacccact cctttcttta tggaccctat ttacagagta 4441 gagaaaagaa aactaactga cccacttgaa gctttaaaag agaaatactt gaggccttct 4501 ccaggattct tgtttcaccc acaattccaa ctgcctgatc agagaacttg gatgtcagct 4561 attgaaaaca tggcagaaaa gctagagagc ttcagtgccc tgaaacctga ggccagtgag 4621 ctcttacagt cagtgccctc tatgttcaac ttcagggcgc ctcccaatgc cctgccagag 4681 aaccttctgc ggaagggaaa ggagcgctat acctgcagat actgtggcaa gatttttcca 4741 aggtctgcaa acctaacacg gcacttgaga acccacacag gagagcagcc ttacagatgc 4801 aaatactgtg acagatcatt tagcatatct tctaacttgc aaaggcatgt tcgcaacatc 4861 cacaataaag agaagccatt taagtgtcac ttatgtgata ggtgttttgg tcaacaaacc 4921 aatttagaca gacacctaaa gaaacatgag aatgggaaca tgtccggtac agcaacatcg 4981 tcgcctcatt ctgaactgga aagtacaggt gcgattctgg atgacaaaga agatgcttac 5041 ttcacagaaa ttcgaaattt cattgggaac agcaaccatg gcagccaatc tcccaggaat 5101 gtggaggaga gaatgaatgg cagtcatttt aaagatgaaa aggctttggt gaccagtcaa 5161 aattcagact tgctggatga tgaagaagtt gaagatgagg tgttgttaga tgaggaggat 5221 gaagacaatg atattactgg aaaaacagga aaggaaccag tgacaagtaa tttacatgaa 5281 ggaaaccctg aggatgacta tgaagaaacc agtgccctgg agatgagttg caagacatcc 5341 ccagtgaggt ataaagagga agaatataaa agtggacttt ctgctctaga tcatataagg 5401 cacttcacag atagcctcaa aatgaggaaa atggaagata atcaatattc tgaagctgag 5461 ctgtcttctt ttagtacttc ccatgtgcca gaggaactta agcagccgtt acacagaaag 5521 tccaaatcgc aggcatatgc tatgatgctg tcactgtctg acaaggagtc cctccattct 5581 acatcccaca gttcttccaa cgtgtggcac agtatggcca gggctgcggc ggaatccagt 5641 gctatccagt ccataagcca cgtaggatcc GACTACAAAG ACCATGACGG TGATTATAAA 5701 GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag gatctgcgat 5761 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 5821 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 5881 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 5941 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 6001 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 6061 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 6121 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 6181 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 6241 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 6301 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 6361 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 6421 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 6481 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 6541 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 6601 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 6661 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 6721 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 6781 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 6841 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 6901 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 6961 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 7021 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 7081 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 7141 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 7201 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 7261 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 7321 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 7381 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 7441 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 7501 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 7561 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 7621 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 7681 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 7741 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 7801 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 7861 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 7921 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 7981 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 8041 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 8101 ttgtcgggga aATCATCGTC CTTTCCTtgg ctgctcgcct gtgttgccac ctggattctg 8161 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 8221 ggcctgctgc cggctctgcg gcctcttccg cgtctTcgcc ttcgccctca gacgagtcgg 8281 atctcccttt ggGccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 8341 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 8401 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 8461 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 8521 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 8581 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 8641 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 8701 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 8761 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 8821 gctaTCCCGC CCCTAACTCC GCCCAtcccg cccctaactc cgcccagttc cgcccattct 8881 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 8941 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 9001 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 9061 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 9121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 9181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 9241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 9301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 9361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 9421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 9481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 9541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 9601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 9661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 9721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 9781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 9841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 9901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 9961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 10021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 10081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 10141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 10201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 10261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 10321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 10381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 10441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 10501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 10561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 10621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 10681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 10741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 10801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 10861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 10921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 10981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 11041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 11101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 11161 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 11221 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 11281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 11341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 11401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 11461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 11521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 11581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.