pCMV-VPS8-mCherry-Neo plasmid

pCMV-VPS8-mCherry-Neo plasmid

PVT87180

pCMV-VPS8-mCherry-Neo plasmid Information

Plasmid name: pCMV-VPS8(human)-mCherry-Neo
Alias: NM_001009921.3;KIAA0804
Gene lenght: 4284bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-2×linker-mCherry-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-VPS8-mCherry-Neo plasmid Description

pCMV-VPS8-mCherry-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the downstream human VPS8 gene. The plasmid carries mCherry red fluorescent protein at the C-terminus of the VPS8 gene, facilitating the visual observation of the location and level of the target gene expression.

pCMV-VPS8-mCherry-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAgcAAC TTAAGGATCC GCCACCatgg aaaatgaacc 901 agaccatgaa aatgtggaac agagcctctg tgccaagacg agcgaagaag agctgaataa 961 gtctttcaat ctagaagctt cactttcaaa attctcttac atagatatgg acaaggaact 1021 ggagttcaaa aatgatctga ttgatgacaa ggagtttgat attcctcaag ttgatactcc 1081 tccaacactg gaaagcatac taaatgagac tgatgatgaa gatgagtctt ttattcttga 1141 ggatcctaca ttgttaaaca ttgatactat tgattctcac tcctatgata cttcatctgt 1201 ggcaagctca gatagtggtg acaggaccaa cttaaaaagg aagaagaaat tacctgattc 1261 tttttcactt catggatcag ttatgcgcca ttcacttttg aagggaattt ctgcccagat 1321 agtgtctgca gctgacaaag tagatgctgg cttgcctaca gcaattgcag tatccagtct 1381 gatagcagtg ggtacatctc atggattggc tttaatattt ggaaaagatc agaatcaagc 1441 tttgcgactc tgtctgggta gcactagtgt tggaggtcag tatggcgcta tctctgccct 1501 cagtatcaac aatgattgct caagacttct ttgtggcttt gctaaaggac agatcaccat 1561 gtgggatttg gccagtggaa aacttctaag atcaataaca gatgctcatc ctccaggaac 1621 agcaatattg catatcaagt ttacagatga tccaactctt gcaatttgca acgacagcgg 1681 aggctctgtt tttgaattga catttaagag agtgatggga gtgagaacct gtgaatctag 1741 atgtctgttc agtggttcca agggtgaagt ctgctgtatt gagcctctgc attctaagcc 1801 tgagttgaaa gatcatccca tcacacagtt ttcattattg gccatggcat ccttgacaaa 1861 aatactggtc attggattga aaccatcctt gaaagtatgg atgacttttc cctatggccg 1921 gatggatcct tccagtgtgc cactgctggc ctggcacttt gtagcagtac aaaattacgt 1981 gaatcccatg cttgccttct gcagaggaga tgttgttcat tttctattgg taaagagaga 2041 tgaatctgga gcaatacatg ttactaagca aaagcatctt cacctatact atgacctcat 2101 caactttacc tggataaatt cacgcacagt tgtgctctta gacagcgtag agaagttgca 2161 tgtgattgat cggcaaacac aagaggaatt ggagacagtg gagatctcag aagttcagct 2221 ggtctacaat agcagccatt tcaaatcact agccactgga ggaaatgtta gccaggcact 2281 ggctttggtt ggagagaagg cttgttatca atccatcagt agctatggtg gtcagatctt 2341 ttatttgggg acaaaatctg tttatgtgat gatgctgagg agctggagag agagagtgga 2401 tcatctcctg aaacaagatt gtcttacaga agcgttggct cttgcgtggt ctttccatga 2461 aggaaaagca aaagcagtag tgggattatc aggggatgcc agtaagcgaa aggctattgt 2521 tgcagaccgg atggtagaaa tcctattcca ttatgcagat cgagctctga aaaagtgccc 2581 agaccaagga aaaatccaag tgatggagca gcattttcag gatatggtgc ctgtcatagt 2641 tgattactgc cttctgctgc agcgaaagga tcttttattt agtcagatgt atgataaatt 2701 aagtgagaat tcagtggcca aaggagtatt tttggagtgc cttgagccat atattttaag 2761 tgataaattg gtgggaatca caccccaagt aatgaaagac ttgattgttc atttccaaga 2821 taaaaaatta atggaaaatg tggaagcgct cattgtacat atggatatca ccagcctaga 2881 tattcagcag gtagttctca tgtgttggga aaatcgttta tatgatgcta tgatctatgt 2941 ctacaacaga ggcatgaatg aatttattag tccaatggag aaacttttca gagtcattgc 3001 tcctcctctg aatgcaggaa aaacactaac agatgaacaa gttgttatgg gcaataagct 3061 ccttgtatat attagctgtt gtctagcagg tcgtgcctat ccccttggtg acatccctga 3121 agatctggtt cccttggtta aaaaccaggt ttttgaattt ctaattcgcc tgcattcagc 3181 agaggcttct cctgaggaag aaatctatcc ttacattcgg actttgctac attttgacac 3241 aagagaattt ctaaatgtat tggcactgac ttttgaagat tttaaaaatg acaagcaagc 3301 tgtggaatat caacagcgaa ttgtggatat tttgttgaaa gttatggtgg agaattcaga 3361 ctttaccccc tcacaagtag gatgtctctt taccttcctt gctcggcagc ttgcaaagcc 3421 tgacaacacc ttgtttgtaa acagaacact ttttgatcag gtccttgaat tcctttgtag 3481 tcctgacgat gactcccgac actctgaaag acagcaggtc cttttagaat tgctgcaggc 3541 tggaggcata gttcaatttg aagagagtcg actcatccgg atggcagaaa aagctgagtt 3601 ctatcaaatt tgtgaattta tgtatgaaag agaacaccaa tatgataaaa ttattgattg 3661 ctacttacgt gaccctctgc gagaggaaga agtctttaat tacattcaca atatcttatc 3721 cattcccgga cacagtgcag aggagaagca gtctgtatgg cagaaagcaa tggatcatat 3781 tgaggaactc gtgtccctga agccttgtaa agctgcggag ctggttgcca cccacttttc 3841 tggacatatt gaaacggtca ttaaaaaact tcagaaccag gttttgcttt tcaaattttt 3901 gaggagtctt cttgacccaa gggaaggtat tcatgtaaat caagaattac tgcaaatatc 3961 tccttgtatc acagagcagt tcattgagct gttgtgtcag ttcaacccaa cccaagttat 4021 agagactctg caagtccttg agtgctaccg tctggaagaa actattcaga ttactcagaa 4081 gtatcaactt catgaagtca ccgcttatct attggaaaag aaaggagata ttcatggtgc 4141 cttcctaata atgttagaga gactacaaag caaacttcaa gaggtaacac atcaaggtga 4201 aaataccaaa gaggatccct cattgaagga tgttgaagat actatggtgg agaccattgc 4261 tctttgccag agaaattcac ataatttgaa ccagcagcaa cgtgaggcac tttggtttcc 4321 gttattggag gcaatgatgg cccctcagaa gctgtccagt tcagccattc ctcatctaca 4381 ctctgaagct ctgaagtctt tgaccatgca agttttaaat agcatggcag catttattgc 4441 ccttccatca atcttgcaaa gaatcttaca ggatccagtt tatggaaaag gaaaacttgg 4501 agaaatccag ggacttatct tgggaatgtt agataccttt aactatgaac aaaccctgct 4561 ggaaacaaca accagccttc taaaccaaga tctccattgg tcattgtgta acctgagagc 4621 ttcggtcacc agaggactga atcccaaaca agattactgc tctatatgtt tgcagcagta 4681 caagagacgc caagaaatgg ctgatgaaat aattgtcttt agctgtggcc atttgtatca 4741 ctcattctgc ctacaaaaca aagaatgcac tgtggaattt gagggccaaa caagatggac 4801 atgctacaaa tgcagttcaa gtaacaaagt aggaaaactc agtgaaaatt catctgaaat 4861 taaaaaggga aggataaccc catcacaggt aaaaatgtct ccatcgtatc atcagtccaa 4921 aggggatccc actgctaaaa agggaacctc agaacctgtt ctggatccac agcaaatcca 4981 agcatttgat cagctttgcc gtctctaccg aggaagctcc aggctggctc tcctcacgga 5041 actctcccag aatcgcagca gcgagagcta taggccattc agtggctcgc agagtgctcc 5101 tgctttcaac agcatcttcc agaatgagaa cttccagctg cagctcattc ctccacctgt 5161 gactgaggat GAATTCggtg gaggcggatc aggaggcggt ggatctatgg tgagcaaggg 5221 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 5281 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 5341 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 5401 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 5461 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 5521 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 5581 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 5641 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 5701 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 5761 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 5821 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 5881 ccactccacc ggcggcatgg acgagctgta caagtagGCG GCCGCTCGAG TCTAGAGGGC 5941 CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT 6001 GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT 6061 AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG 6121 TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG 6181 TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG 6241 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 6301 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 6361 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 6421 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 6481 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 6541 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 6601 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 6661 CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC 6721 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC 6781 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT 6841 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 6901 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT 6961 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG 7021 AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT 7081 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA 7141 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA 7201 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA 7261 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA 7321 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT 7381 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG 7441 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA 7501 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA 7561 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA 7621 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG 7681 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC 7741 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT 7801 GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA 7861 GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA 7921 TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC 7981 CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC 8041 CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC 8101 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA 8161 TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG 8221 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 8281 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 8341 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 8401 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 8461 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 8521 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 8581 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 8641 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 8701 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 8761 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 8821 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 8881 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 8941 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 9001 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 9061 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 9121 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG 9181 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 9241 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 9301 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 9361 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 9421 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 9481 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA 9541 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 9601 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 9661 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 9721 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 9781 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 9841 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 9901 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 9961 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 10021 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 10081 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 10141 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 10201 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 10261 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 10321 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA 10381 GATCTCCCGA TCCCCTATGG T

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.