pCMV-Bicc1-3×FLAG-Neo plasmid

pCMV-Bicc1-3×FLAG-Neo plasmid

PVT98343

pCMV-Bicc1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Bicc1(mouse)-3×FLAG-Neo
Alias: NM_031397.3;Bic-C; bpk; jcpk
Gene lenght: 2931bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Bicc1-3×FLAG-Neo plasmid Description

pCMV-Bicc1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Bicc1-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC atggcctcgc agagcgagcc 961 gggctacctg gcggcggcgc agtcggaccc cggctccaac agcgagcgca gcaccgactc 1021 gccggtggcc ggctccgagg acgatctggt ggccgcggcg cccctcttgc acagcccgga 1081 gtggagcgag gagcgcttcc gcgtggacag gaagaaactc gaggccatgc tccaagctgc 1141 agctgaagga aaaggccgaa gtggggaaga cttttttcag aagatcatgg aggagacaaa 1201 cacgcagatt gcatggccgt ccaaactgaa gatcggggct aaatccaaga aagatcccca 1261 catcaaggtt tctgggaaga aagaggatgt gaaggaagcc aaagaaatga tcatgtctgt 1321 cttagacaca aaaagcaacc gcgtcacatt gaagatggat gtctcgcaca cggagcactc 1381 ccacgtcatc ggcaagggtg gtaacaacat taaaaaggtc atggaagaca cgggctgcca 1441 catccacttc ccagactcca acaggaacaa ccaggcagag aagagtaacc aggtgtctat 1501 agcaggacag ccagcaggag tagaatcggc ccgagcaagg attcgggagc tgcttccttt 1561 ggtgctgatg tttgagttac cgattgccgg gattctccag ccagtccccg atcccaacac 1621 cccgtccatt cagcacatct cacaaaccta cagcgtttct gtgtccttta agcagaggtc 1681 tcgaatgtat ggtgctacag tcacagtacg aggctctcag aataacacta atgctgtgaa 1741 ggaaggaaca gccatgctgt tggaacacct tgcgggaagc ttggcctccg ccatccccgt 1801 gagcacacaa ctggacatag cagcccagca tcacctcttc atgatgggcc ggaacgggag 1861 caacgtcaaa cacatcatgc agaggacagg ggcgcagatt cactttcccg accccagcaa 1921 tccacagaag aaatccaccg tctacctcca gggcaccatt gagtctgtct gcctagcaag 1981 gcagtatctc atggggtgtc ttcctctggt gttgatgttt gatatgaagg aagacattga 2041 agtggaccca caggtcatcg cacagctgat ggaacagctg gacgtcttta tcagtattaa 2101 accaaagccc aaacagccga gcaagtctgt gattgtgaaa agtgttgagc gaaatgcctt 2161 aaatatgtat gaagcaagga agtgtctcct cggacttgaa agcagtgggg tttccatagc 2221 aaccagtcta tccccagcat cgtgccctgc cggcctggcc tgtcccagcc tggatatctt 2281 agcttcggca ggcctcggac tcactggact aggtttattg gggcccacca cattgtcgct 2341 caatacgtca gccaccccaa actcactcct gaatgctctc aacacttcgg tcagtccttt 2401 gcaaagttca agttctggta ctcccagtcc tacactgtgg gcacccccaa tcgctaacac 2461 tgcaagcgcc acaggtttct ctacgatacc acaccttatg cttccctcta ctgcccaggc 2521 cacattaacc aatattttgc tgtctggagt gcccacatac gggcacacgg ctccatctcc 2581 cccacctggc ttgactcctg ttgatgttca catcaacagc atgcagacag aaggcaaaaa 2641 catctctgcg tctataaatg gacatgtgca gcctgcaaac atgaaatacg gtccgctgtc 2701 cacttcatcg cttggggaaa aagtgctgag ttcgaatcat ggtgacccat ccatgcagac 2761 agctgggccc gaacaggctt ctcctaaatc aaactcggtg gaaggctgca atgatgcctt 2821 tgttgaagtg ggcatgcctc gaagtccctc ccattctgga aacgctggcg acttgaagca 2881 gatgctgggt gcctccaagg tctcctgtgc caagcggcag acggttgagc tactgcaggg 2941 cacgaagaac tcgcacctcc acggcactga cagactactc tcagaccctg aactgagcgc 3001 cacagaaagt ccgcttgctg acaagaaggc cccggggagc gaacgtgcag ctgagagggc 3061 agcagctgcc cagcagaaat cggagagggc ccgcctggcc tcgcagccaa catatgtcca 3121 catgcaggca tttgattatg agcagaagaa actattagcc accaaagcga tgttaaagaa 3181 gccagtggtg actgaggtca gaacacctac gaatacgtgg agtggcctgg gattctcaaa 3241 gtccatgccg gcagaaacca ttaaggaact gaggagagcc aaccacgtat cctataagcc 3301 cacgatgacc accgcctatg agggctcctc attgtccctc tcaaggtcca gcagtcgtga 3361 gcacctggca agtggaagcg agtcagacaa ctggagagac cggaatggaa taggccccat 3421 gggtcacagt gaattctcag caccaatcgg cagccccaag cgcaagcaga acaaatcaag 3481 agagcactat ctaagcagca gcaactacat ggactgcatt tcctcgctga cgggaagcaa 3541 tggctgtaac ctgaacagct gcttcaaagg ctccgacctc cccgagcttt tcagcaagct 3601 gggcctaggc aaatacacgg atgtcttcca gcagcaagag atcgatcttc agacattcct 3661 caccctcaca gatcaggatc tgaaggagct gggaatcaca acctttggtg cccgaaggaa 3721 aatgctgctg gcaatctcag agctaagtaa aaaccgaaga aaactttttg aaccaccaaa 3781 cgcatcatgc acctccttcc tggaaggcgg agccagtggg aggctgcctc gccagtatca 3841 ttcagacatt gcgagcgtca gtggccgctg gCTCGAGGAC TACAAAGACC ATGACGGTGA 3901 TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG GGCCCGTTTA 3961 AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 4021 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 4081 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 4141 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC 4201 TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG 4261 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 4321 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 4381 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 4441 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 4501 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 4561 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 4621 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA 4681 ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 4741 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 4801 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 4861 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 4921 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 4981 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 5041 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 5101 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 5161 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 5221 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 5281 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 5341 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 5401 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 5461 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 5521 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 5581 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 5641 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 5701 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 5761 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 5821 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 5881 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 5941 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 6001 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 6061 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 6121 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 6181 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 6241 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 6301 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 6361 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 6421 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 6481 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 6541 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 6601 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 6661 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 6721 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 6781 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 6841 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 6901 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 6961 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 7021 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 7081 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 7141 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 7201 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 7261 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 7321 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 7381 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 7441 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 7501 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 7561 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 7621 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 7681 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 7741 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 7801 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 7861 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 7921 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 7981 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 8041 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 8101 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 8161 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 8221 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 8281 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 8341 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT C

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.