pCMV-ENO1-P2A-mCherry-Neo plas

pCMV-ENO1-P2A-mCherry-Neo plasmid

PVT97326

pCMV-ENO1-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-ENO1(human)-P2A-mCherry-Neo
Alias: NM_001428.5;ENO1-IT1; ENO1L1; HEL-S-17; MPB1; NNE; PPH
Gene lenght: 1302bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ENO1-P2A-mCherry-Neo plasmid Description

pCMV-ENO1-P2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ENO1-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccGC CACCatgtct 781 attctcaaga tccatgccag ggagatcttt gactctcgcg ggaatcccac tgttgaggtt 841 gatctcttca cctcaaaagg tctcttcaga gctgctgtgc ccagtggtgc ttcaactggt 901 atctatgagg ccctagagct ccgggacaat gataagactc gctatatggg gaagggtgtc 961 tcaaaggctg ttgagcacat caataaaact attgcgcctg ccctggttag caagaaactg 1021 aacgtcacag aacaagagaa gattgacaaa ctgatgatcg agatggatgg aacagaaaat 1081 aaatctaagt ttggtgcgaa cgccattctg ggggtgtccc ttgccgtctg caaagctggt 1141 gccgttgaga agggggtccc cctgtaccgc cacatcgctg acttggctgg caactctgaa 1201 gtcatcctgc cagtcccggc gttcaatgtc atcaatggcg gttctcatgc tggcaacaag 1261 ctggccatgc aggagttcat gatcctccca gtcggtgcag caaacttcag ggaagccatg 1321 cgcattggag cagaggttta ccacaacctg aagaatgtca tcaaggagaa atatgggaaa 1381 gatgccacca atgtggggga tgaaggcggg tttgctccca acatcctgga gaataaagaa 1441 ggcctggagc tgctgaagac tgctattggg aaagctggct acactgataa ggtggtcatc 1501 ggcatggacg tagcggcctc cgagttcttc aggtctggga agtatgacct ggacttcaag 1561 tctcccgatg accccagcag gtacatctcg cctgaccagc tggctgacct gtacaagtcc 1621 ttcatcaagg actacccagt ggtgtctatc gaagatccct ttgaccagga tgactgggga 1681 gcttggcaga agttcacagc cagtgcagga atccaggtag tgggggatga tctcacagtg 1741 accaacccaa agaggatcgc caaggccgtg aacgagaagt cctgcaactg cctcctgctc 1801 aaagtcaacc agattggctc cgtgaccgag tctcttcagg cgtgcaagct ggcccaggcc 1861 aatggttggg gcgtcatggt gtctcatcgt tcgggggaga ctgaagatac cttcatcgct 1921 gacctggttg tggggctgtg cactgggcag atcaagactg gtgccccttg ccgatctgag 1981 cgcttggcca agtacaacca gctcctcaga attgaagagg agctgggcag caaggctaag 2041 tttgccggca ggaacttcag aaaccccttg gccaaggaat tcggaagcgg agctactaac 2101 ttcagcctgc tgaagcaggc tggcgacgtg gaggagaacc ctggacctat ggtgagcaag 2161 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 2221 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 2281 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 2341 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 2401 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 2461 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc 2521 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 2581 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 2641 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 2701 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg 2761 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 2821 cgccactcca ccggcggcat ggacgagctg tacaagtaac tcgagtctag agggcccctt 2881 aagggttcga tccctaccgg ttagtaatga gtttgatatc tcgacaatca acctctggat 2941 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 3001 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 3061 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 3121 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 3181 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 3241 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 3301 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 3361 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 3421 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 3481 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa acgggggagg ctaactgaaa 3541 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 3601 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 3661 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 3721 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 3781 ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt 3841 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 3901 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 3961 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg 4021 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 4081 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4141 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4201 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 4261 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 4321 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 4381 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 4441 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 4501 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 4561 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 4621 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 4681 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 4741 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 4801 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 4861 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 4921 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 4981 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 5041 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 5101 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 5161 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 5221 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 5281 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 5341 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 5401 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 5461 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga 5521 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 5581 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 5641 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 5701 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 5761 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 5821 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 5881 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 5941 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 6001 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 6061 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 6121 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 6181 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 6241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 6301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 6361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg 6421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 6481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 6541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 6601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 6661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 6721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 6781 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 6841 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 6901 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 6961 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 7021 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7081 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 7141 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7201 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 7261 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 7321 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7381 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7441 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7501 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7561 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7621 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7681 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7741 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7801 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 7861 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 7921 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 7981 ctcccgatcc cctatggtcg actctcagta caatctgctc tgatgccgca tagttaagcc 8041 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag 8101 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.