pLV3-CMV-Slc16a1-CopGFP-Puro p

pLV3-CMV-Slc16a1-CopGFP-Puro plasmid

PVT97266

pLV3-CMV-Slc16a1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Slc16a1(mouse)-CopGFP-Puro
Alias: NM_009196.4;Mct1
Gene lenght: 1479bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Slc16a1-CopGFP-Puro plasmid Description

pLV3-CMV-Slc16a1-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Slc16a1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgccacc tgcgattgga gggccagtgg gatacacccc 2341 cccagatgga ggctgggggt gggcagtgtt agtcggagcc ttcatttcta ttggcttctc 2401 ctatgcattt cccaaatcca tcactgtctt ctttaaagag atagaagtta tattcagtgc 2461 aacgaccagt gaagtatcat ggatatcatc tataatgttg gctgtcatgt atgctggagg 2521 tcctatcagc agtatcttgg tgaataaata cggcagccgt ccagtaatga tcgctggtgg 2581 ttgtctgtct ggttgcggct tgatcgcagc ttctttctgt aacacagtac aggaacttta 2641 cttgtgcatt ggtgttattg gaggtcttgg gcttgctttc aacttgaacc cagctctgac 2701 tatgattggc aagtatttct acaagaagcg accactggcc aacggactgg ccatggcagg 2761 cagccctgtg ttcctctcta ccctggctcc acttaatcag gctttctttg atatttttga 2821 ctggagagga agcttcctaa ttcttggggg cctcctccta aattgttgtg tagctggatc 2881 cctgatgaga ccaatagggc ctgagcaagt caagctagaa aaactcaagt ccaaagaatc 2941 tctacaggaa gctggaaaat ctgatgcaaa tacagatctc attggaggaa gtcccaaagg 3001 agaaaagctg tccgtcttcc aaacaattaa caaattcctg gacttgtcgc tgtttaccca 3061 taggggcttt ttactgtacc tgtctggaaa tgtggtcatg ttttttggac tctttacccc 3121 tttggtcttt cttagtagtt atggtaagag taaggatttt tccagtgaga aatcagcctt 3181 ccttctttcc attttggctt ttgttgatat ggtagccaga ccgtccatgg gacttgcagc 3241 caacaccaag tggatcagac ctcggatcca gtactttttt gctgcttctg ttgttgcaaa 3301 tggagtgtgc catttgcttg cccctttgtc tacaacctac gttgggttct gtgtctacgc 3361 cggagtcttt ggatttgcct ttggttggct cagctctgta ttatttgaaa cattgatgga 3421 cctcattgga ccccagaggt tctccagtgc tgtgggcttg gtgaccattg tggaatgctg 3481 ccctgtcctc ctagggccac cacttttagg ccgcctcaat gacatgtatg gagactacaa 3541 atacacgtac tgggcttgtg gcgtgatcct catcatcgcg ggtatctatc tcttcattgg 3601 catgggcatc aactatcgac ttcttgccaa agaacagaaa gcggaggaga agcagaaaag 3661 ggaaggaaaa gaggacgagg ccagcaccga tgtcgacgag aagccaaagg agacgatgaa 3721 agctgcacag tcgccgcagc agcacagctc cggggacccc acagaggagg agagccctgt 3781 cTAggatccg cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg 3841 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta 3901 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc 3961 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa 4021 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc 4081 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc 4141 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc 4201 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg 4261 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag 4321 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct 4381 gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct 4441 ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac 4501 caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta 4561 ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg 4621 cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt 4681 cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt 4741 ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca 4801 cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg 4861 cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca 4921 ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca 4981 cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt 5041 cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc 5101 caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc 5161 cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 5221 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 5281 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 5341 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 5401 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 5461 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 5521 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 5581 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 5641 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 5701 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 5761 tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5821 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5881 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5941 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 6001 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6061 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6121 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 6181 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6241 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6301 gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt 6361 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6421 ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac 6481 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6541 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6601 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6661 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6721 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6841 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc 6901 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 6961 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 7021 agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 7081 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 7141 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 7201 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 7261 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 7321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 7381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 7441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 7501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 7561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 7621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 7681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7861 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 7921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 8041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 8101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 8161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 8221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 8281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 8341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 8401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 8461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 8521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 8581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 8641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 8701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 8761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 8821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 8881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 8941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 9001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 9061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 9121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 9181 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 9241 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc 9301 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 9361 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg 9421 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 9481 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa 9541 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca 9601 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc 9661 agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.