pLV3-CMV-BUB1B-3×FLAG-CopGFP-P

pLV3-CMV-BUB1B-3×FLAG-CopGFP-Puro plasmid

PVT97225

pLV3-CMV-BUB1B-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-BUB1B(human)-3×FLAG-CopGFP-Puro
Alias: NM_001211.6;Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L;MVA1; SSK1
Gene lenght: 3150bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-BUB1B-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-BUB1B-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-BUB1B-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cggcggtgaa gaaggaaggg 2341 ggtgctctga gtgaagccat gtccctggag ggagatgaat gggaactgag taaagaaaat 2401 gtacaacctt taaggcaagg gcggatcatg tccacgcttc agggagcact ggcacaagaa 2461 tctgcctgta acaatactct tcagcagcag aaacgggcat ttgaatatga aattcgattt 2521 tacactggaa atgaccctct ggatgtttgg gataggtata tcagctggac agagcagaac 2581 tatcctcaag gtgggaagga gagtaatatg tcaacgttat tagaaagagc tgtagaagca 2641 ctacaaggag aaaaacgata ttatagtgat cctcgatttc tcaatctctg gcttaaatta 2701 gggcgtttat gcaatgagcc tttggatatg tacagttact tgcacaacca agggattggt 2761 gtttcacttg ctcagttcta tatctcatgg gcagaagaat atgaagctag agaaaacttt 2821 aggaaagcag atgcgatatt tcaggaaggg attcaacaga aggctgaacc actagaaaga 2881 ctacagtccc agcaccgaca attccaagct cgagtgtctc ggcaaactct gttggcactt 2941 gagaaagaag aagaggagga agtttttgag tcttctgtac cacaacgaag cacactagct 3001 gaactaaaga gcaaagggaa aaagacagca agagctccaa tcatccgtgt aggaggtgct 3061 ctcaaggctc caagccagaa cagaggactc caaaatccat ttcctcaaca gatgcaaaat 3121 aatagtagaa ttactgtttt tgatgaaaat gctgatgagg cttctacagc agagttgtct 3181 aagcctacag tccagccatg gatagcaccc cccatgccca gggccaaaga gaatgagctg 3241 caagcaggcc cttggaacac aggcaggtcc ttggaacaca ggcctcgtgg caatacagct 3301 tcactgatag ctgtacccgc tgtgcttccc agtttcactc catatgtgga agagactgca 3361 cgacagccag ttatgacacc atgtaaaatt gaacctagta taaaccacat cctaagcacc 3421 agaaagcctg gaaaggaaga aggagatcct ctacaaaggg ttcagagcca tcagcaagcg 3481 tctgaggaga agaaagagaa gatgatgtat tgtaaggaga agatttatgc aggagtaggg 3541 gaattctcct ttgaagaaat tcgggctgaa gttttccgga agaaattaaa agagcaaagg 3601 gaagccgagc tattgaccag tgcagagaag agagcagaaa tgcagaaaca gattgaagag 3661 atggagaaga agctaaaaga aatccaaact actcagcaag aaagaacagg tgatcagcaa 3721 gaagagacga tgcctacaaa ggagacaact aaactgcaaa ttgcttccga gtctcagaaa 3781 ataccaggaa tgactctatc cagttctgtt tgtcaagtaa actgttgtgc cagagaaact 3841 tcacttgcgg agaacatttg gcaggaacaa cctcattcta aaggtcccag tgtacctttc 3901 tccatttttg atgagtttct tctttcagaa aagaagaata aaagtcctcc tgcagatccc 3961 ccacgagttt tagctcaacg aagacccctt gcagttctca aaacctcaga aagcatcacc 4021 tcaaatgaag atgtgtctcc agatgtttgt gatgaattta caggaattga acccttgagc 4081 gaggatgcca ttatcacagg cttcagaaat gtaacaattt gtcctaaccc agaagacact 4141 tgtgactttg ccagagcagc tcgttttgta tccactcctt ttcatgagat aatgtccttg 4201 aaggatctcc cttctgatcc tgagagactg ttaccggaag aagatctaga tgtaaagacc 4261 tctgaggacc agcagacagc ttgtggcact atctacagtc agactctcag catcaagaag 4321 ctgagcccaa ttattgaaga cagtcgtgaa gccacacact cctctggctt ctctggttct 4381 tctgcctcgg ttgcaagcac ctcctccatc aaatgtcttc aaattcctga gaaactagaa 4441 cttactaatg agacttcaga aaaccctact cagtcaccat ggtgttcaca gtatcgcaga 4501 cagctactga agtccctacc agagttaagt gcctctgcag agttgtgtat agaagacaga 4561 ccaatgccta agttggaaat tgagaaggaa attgaattag gtaatgagga ttactgcatt 4621 aaacgagaat acctaatatg tgaagattac aagttattct gggtggcgcc aagaaactct 4681 gcagaattaa cagtaataaa ggtatcttct caacctgtcc catgggactt ttatatcaac 4741 ctcaagttaa aggaacgttt aaatgaagat tttgatcatt tttgcagctg ttatcaatat 4801 caagatggct gtattgtttg gcaccaatat ataaactgct tcacccttca ggatcttctc 4861 caacacagtg aatatattac ccatgaaata acagtgttga ttatttataa ccttttgaca 4921 atagtggaga tgctacacaa agcagaaata gtccatggtg acttgagtcc aaggtgtctg 4981 attctcagaa acagaatcca cgatccctat gattgtaaca agaacaatca agctttgaag 5041 atagtggact tttcctacag tgttgacctt agggtgcagc tggatgtttt taccctcagc 5101 ggctttcgga ctgtacagat cctggaagga caaaagatcc tggctaactg ttcttctccc 5161 taccaggtag acctgtttgg tatagcagat ttagcacatt tactattgtt caaggaacac 5221 ctacaggtct tctgggatgg gtccttctgg aaacttagcc aaaatatttc tgagctaaaa 5281 gatggtgaat tgtggaataa attctttgtg cggattctga atgccaatga tgaggccaca 5341 gtgtctgttc ttggggagct tgcagcagaa atgaatgggg tttttgacac tacattccaa 5401 agtcacctga acaaagcctt atggaaggta gggaagttaa ctagtcctgg ggctttgctc 5461 tttcagggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 5521 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 5581 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 5641 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 5701 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 5761 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 5821 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 5881 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 5941 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 6001 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 6061 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 6121 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 6181 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 6241 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 6301 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 6361 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 6421 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 6481 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 6541 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 6601 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 6661 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 6721 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 6781 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 6841 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 6901 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 6961 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 7021 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 7081 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 7141 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 7201 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 7261 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 7321 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 7381 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 7441 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 7501 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 7561 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 7621 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 7681 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 7741 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 7801 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 7861 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 7921 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 7981 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 8041 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 8101 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 8161 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 8221 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 8281 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 8341 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 8401 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 8461 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 8521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 8581 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 8641 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 8701 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 8761 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 8821 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 8881 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 8941 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 9001 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 9061 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 9121 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 9181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 9241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 9301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 9361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 9421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 9481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 9541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 9601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 9661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 9721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 9781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 9841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 9901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 9961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 10021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 10081 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 10141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 10201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 10261 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 10321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 10381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 10441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 10501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 10561 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 10621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 10681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 10741 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 10801 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 10861 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 10921 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 10981 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 11041 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 11101 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 11161 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 11221 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 11281 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 11341 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 11401 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.