pLV3-CMV-Nln-3×FLAG-CopGFP-Pur

pLV3-CMV-Nln-3×FLAG-CopGFP-Puro plasmid

PVT97212

pLV3-CMV-Nln-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Nln(mouse)-3×FLAG-CopGFP-Puro
Alias: NM 029447.2;4930472G13Rik
Gene lenght: 2112bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Nln-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Nln-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Nln-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tcaccctgtg cctttcggct 2341 ctgcgaggcc tccacagagc tggtggttcc aggattcggc tcagaatgac gttggggagg 2401 gaggcggcat ctcctcttca ggccatgtct tcctacaccg ccgcaggcag gaatgttctc 2461 agatgggacc tttctccaga gcagatcagg acgaggacag aggagctcat agcacagacc 2521 aagcaggtgt atgataccgt ggggacaatc aacctggagg atgtaactta cgagaactgt 2581 ctgcaggtgc tggctgacat agaagtgaag tacatagtgg aaaggaccat gctggacttc 2641 cctcagcacg tgtcgtctga cagagaagtg cgggctgcaa gcacagaagc ggacaaaagg 2701 ctttctcgtt ttgatattga gatgagcatg agagaagatg tatttcagag aattgttcat 2761 ttacaagaaa cgtgcgattt ggagaagata aagcctgagg ccaggcgata cttggaaaaa 2821 tcaattaaaa tggggaaaag gaatggactc catcttccag aacacgtaaa aaatgaaatc 2881 aaatcaatga agaagagaat gagtgagcta tgcattgact tcaacaaaaa cctcaatgag 2941 gatgacacgt cccttgtatt ttccaaggct gaacttggtg ctcttcccga cgacttcatt 3001 gacagtttag aaaaaacaga tgaagacaag tacaaagtta ccttaaagta tccccactat 3061 tttcctgtca tgaagaaatg ctgtgtccct gaaactcgaa ggaagatgga aatggctttt 3121 cataccaggt gcaaagagga aaacaccata attctgcaac agctcctgcc gctgcgagca 3181 caagtggcca aactgctcgg ctacaacacc catgctgact ttgtccttga actgaacact 3241 gcaaagagca caagccatgt ggcaaccttt ctagatgatt taagccagaa attaaaaccc 3301 ttgggtgagg ctgaacggga gtttattttg agcttgaaga agaaggaatg cgaagagcga 3361 ggttttgcat acgacgggaa gatcaacgca tgggacctcc attactacat gacccagaca 3421 gaggagctca agtactctgt ggaccaggag tccctcaagg agtactttcc cattgaggtg 3481 gtcactgaag gcctgctaag catctaccag gagctgttgg gactttcatt tgaacaagtg 3541 gctgatgccc atgtttggaa taagagtgtt tcactttaca ccgtgaagga taaagctact 3601 ggagaagtgc tggggcagtt ctacctggac ctctatccaa gggaaggaaa atacaaccat 3661 gcggcctgct ttggtctcca gccaggctgc cttctccctg atggcagtcg gatgatgtct 3721 gtggctgccc tggtggtcaa cttctctcag cccatagcag gccggccctc tctcctgaga 3781 cacgacgaag tgcgaactta cttccatgag tttggtcacg tcatgcatca gatctgtgca 3841 cagactgact ttgcacgatt tagtggaaca aatgtggaaa ctgactttgt agaggtgcca 3901 tcacaaatgc ttgaaaactg ggtgtgggac attgactccc tgcgaaaact gtcaaaacat 3961 tatagagacg gacaccctat cacagacgag ctgctggaga agcttgtggc ttcgagactg 4021 gtcaacacag gtcttctgac gcttcgccaa attgttttga gcaaagttga ccagtccctc 4081 cataccaatg cgtcgctgga tgccgcgagc gaatacgcta aatactgcac agaaattttg 4141 ggagttgcag ctacgccagg cacaaatatg ccagctacct ttggacattt ggcaggaggg 4201 tatgacggcc agtattatgg atatctttgg agtgaagtct tttccatgga catgtttcac 4261 agctgtttca gaaaagaagg gattatgaat ccagaggttg gaatgaaata cagaaaccta 4321 atcctgaagc ctggggggtc cctggacggc atggacatgc tccagaattt cttgcaacgt 4381 gagccaaacc agaaagcatt cctgatgagt cgaggcctga atgcttcggg atccGACTAC 4441 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 4501 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4561 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4621 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4681 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4741 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4801 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4861 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4921 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4981 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 5041 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 5101 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5161 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5221 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 5281 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 5341 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5401 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5461 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5521 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5581 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5641 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5701 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5761 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5821 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5881 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5941 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6001 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6061 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6121 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6181 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6241 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6301 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6361 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6421 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6481 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6541 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6601 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6661 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6721 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6781 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6841 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 6901 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6961 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 7021 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 7081 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 7141 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 7201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 7381 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 7441 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 7501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 7561 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 7621 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 7681 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 7741 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 7801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 8341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 8401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8581 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 8641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 9301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 9361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9901 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9961 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 10021 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 10081 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 10141 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 10201 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 10261 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 10321 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 10381 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.