pLV2-CMV-GOLIM4-EGFP-IRES-Puro

pLV2-CMV-GOLIM4-EGFP-IRES-Puro plasmid

PVT97186

pLV2-CMV-GOLIM4-EGFP-IRES-Puro plasmid Information

Plasmid name: pLV2-CMV-GOLIM4(human)-EGFP-IRES-Puro
Alias: NM_014498.5;GIMPC; GOLPH4; GPP130; P138，Inducing agents should be added during extraction
Gene lenght: 2088bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): EPI400
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV2-CMV-GOLIM4-EGFP-IRES-Puro plasmid Description

pLV2-CMV-GOLIM4-EGFP-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-GOLIM4-EGFP-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcGC CACCatggga 2821 aacgggatgt gctcccgaaa gcagaagcgg attttccaga cgctgctgct gctgaccgtc 2881 gtgttcggct ttctctacgg cgcgatgctc tactacgagc tgcagacgca gctgcggaaa 2941 gccgaggcgg tggcgctcaa gtaccagcag caccaggagt ccctctccgc ccagttacaa 3001 gttgtatatg aacacagatc aagattagag aaatccttgc aaaaagaaag acttgaacat 3061 aaaaaagcaa aggaagattt tcttgtttat aagttagaag cacaagaaac attaaataaa 3121 ggaaggcaag attccaatag cagatacagt gcactgaatg tccaacatca gatgttgaaa 3181 agccaacacg aggagctaaa gaaacagcac agtgacttgg aagaggaaca tcgcaaacaa 3241 ggggaagact tcagtagaac atttaatgac cataagcaaa aatacttgca gctccagcaa 3301 gaaaaagaac aagaactttc taagctaaaa gagactgtat acaatttgag agaagagaat 3361 agacaactaa ggaaagcaca ccaagacata catacacagc ttcaagatgt caagcaacag 3421 cataagaatt tactctccga gcatgaacaa cttgtagtga ctttggaaga ccacaagagt 3481 gcactagctg ctgcacagac tcaagttgca gaatataaac aactgaaaga tactctgaat 3541 aggattccaa gccttcgaaa acctgatcca gcagaacagc aaaatgtgac ccaggtggca 3601 cattctccac aaggttacaa cacagcaagg gagaagccaa cccgagaggt gcaggaggtg 3661 tctcgaaata atgatgtgtg gcagaaccat gaagcagttc ctggaagagc agaagacaca 3721 aaactctatg ctcccaccca taaggaggca gaatttcagg ctcccccaga gccaatccaa 3781 caagaagtgg aacgcagaga acctgaggag catcaggtgg aagaggagca cagaaaggcc 3841 ctggaggagg aagaaatgga gcaggtcggg caagcagaac atcttgagga ggaacacgat 3901 ccatcaccag aggagcagga tcgggagtgg aaagagcagc atgagcaacg agaagcagcc 3961 aacctcctgg aagggcacgc gcgtgctgag gtgtaccctt cagccaagcc aatgatcaaa 4021 ttccaatcac cctatgagga acagttggaa cagcagagac tggcagtgca gcaggtggag 4081 gaggcccagc agctgcggga acaccaggaa gctttgcacc agcagaggct gcaggggcac 4141 ttactacggc agcaggaaca gcagcagcag caggtggcaa gagagatggc cctgcagagg 4201 caggctgagc ttgaggaggg ccggccgcag caccaggagc agctccggca gcaagctcat 4261 tatgatgcta tggataatga tatcgttcag ggagcagagg accagggaat ccaaggagag 4321 gaaggagcct atgaaagaga caaccagcac caagatgaag cagaaggaga tccaggtaat 4381 agacatgagc ctcgtgaaca aggaccccga gaagccgacc cagaatctga ggcagatagg 4441 gcagctgtag aagatataaa cccagcagat gaccctaata atcaaggtga ggatgaattt 4501 gaagaagccg agcaagtgag agaagaaaat ttgccagatg aaaatgaaga gcaaaaacaa 4561 agtaatcaaa agcaagagaa tacagaagtg gaggaacatt tggtgatggc aggaaatcca 4621 gaccagcagg aggacaatgt tgatgaacag taccaggaag aggcagaaga ggaggttcag 4681 gaagatttga ctgaagagaa aaaaagggaa ctggagcata atgctgaaga gacctatggt 4741 gaaaatgatg aaaatactga tgataaaaat aatgatggag aagagcaaga agttcgagat 4801 gacaaccgcc ccaaaggccg agaggaacac tacgaggagg aagaagagga ggaagaagac 4861 ggggctgcag ttgctgagaa atcacatcga agagctgaaa tgctcgagGG CGGTGGAGGA 4921 AGCggcggag gtggatctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 4981 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 5041 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 5101 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 5161 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 5221 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 5281 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 5341 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 5401 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 5461 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 5521 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 5581 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 5641 gacgagctgt acaagtagac tagttctaga gcggccgcgg atcccgcccc tctccctccc 5701 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 5761 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 5821 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 5881 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 5941 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 6001 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 6061 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 6121 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat 6181 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 6241 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga 6301 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 6361 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 6421 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 6481 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 6541 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 6601 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 6661 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 6721 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 6781 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 6841 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct 6901 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 6961 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 7021 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 7081 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 7141 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 7201 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 7261 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 7321 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 7381 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 7441 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 7501 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc 7561 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt 7621 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 7681 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct 7741 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg 7801 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc 7861 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct 7921 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc 7981 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc 8041 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg 8101 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc 8161 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 8221 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 8281 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 8341 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 8401 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat 8461 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 8521 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 8581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 8641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 8701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 8761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 8821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 8881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 8941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 9001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 9061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 9121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 9181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 9241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 9301 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 9361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 9421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 9481 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 9541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 9601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 9661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 9721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 9781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 9841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 9901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 9961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 10021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 10081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 10141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 10201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 10261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 10321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 10381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 10441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 10501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 10561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 10621 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 10681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 10741 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 10801 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 10861 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 10921 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag 10981 tagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.