pLV3-CMV-8×His-TRAF2-mCherry-B

pLV3-CMV-8×His-TRAF2-mCherry-Blast plasmid

PVT97069

pLV3-CMV-8×His-TRAF2-mCherry-Blast plasmid Information

Plasmid name: pLV3-CMV-8×His-TRAF2(human)-mCherry-Blast
Alias: NM_021138.4;MGC:45012; RNF117; TRAP; TRAP3
Gene lenght: 1503bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-8×His-TRAF2-mCherry-Blast plasmid Description

pLV3-CMV-8×His-TRAF2-mCherry-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-8×His-TRAF2-mCherry-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAgccacca tgCACCATCA CCATCACCAT CACCATatgg ctgcagctag cgtgaccccc 2341 cctggctccc tggagttgct acagcccggc ttctccaaga ccctcctggg gaccaagctg 2401 gaagccaagt acctgtgctc cgcctgcaga aacgtcctcc gcaggccctt ccaggcgcag 2461 tgtggccacc ggtactgctc cttctgcctg gccagcatcc tcagctctgg gcctcagaac 2521 tgtgctgcct gtgttcacga gggcatatat gaagaaggca tttctatttt agaaagcagt 2581 tcggccttcc cagataatgc tgcccgcagg gaggtggaga gcctgccggc cgtctgtccc 2641 agtgatggat gcacctggaa ggggaccctg aaagaatacg agagctgcca cgaaggccgc 2701 tgcccgctca tgctgaccga atgtcccgcg tgcaaaggcc tggtccgcct tggtgaaaag 2761 gagcgccacc tggagcacga gtgcccggag agaagcctga gctgccggca ttgccgggca 2821 ccctgctgcg gagcagacgt gaaggcgcac cacgaggtct gccccaagtt ccccttaact 2881 tgtgacggct gcggcaagaa gaagatcccc cgggagaagt ttcaggacca cgtcaagact 2941 tgtggcaagt gtcgagtccc ttgcagattc cacgccatcg gctgcctcga gacggtagag 3001 ggtgagaaac agcaggagca cgaggtgcag tggctgcggg agcacctggc catgctactg 3061 agctcggtgc tggaggcaaa gcccctcttg ggagaccaga gccacgcggg gtcagagctc 3121 ctgcagaggt gcgagagcct ggagaagaag acggccactt ttgagaacat tgtctgcgtc 3181 ctgaaccggg aggtggagag ggtggccatg actgccgagg cctgcagccg gcagcaccgg 3241 ctggaccaag acaagattga agccctgagt agcaaggtgc agcagctgga gaggagcatt 3301 ggcctcaagg acctggcgat ggctgacttg gagcagaagg tcttggagat ggaggcatcc 3361 acctacgatg gggtcttcat ctggaagatc tcagacttcg ccaggaagcg ccaggaagct 3421 gtggctggcc gcatacccgc catcttctcc ccagccttct acaccagcag gtacggctac 3481 aagatgtgtc tgcgtatcta cctgaacggc gacggcaccg ggcgaggaac acacctgtcc 3541 ctcttctttg tggtgatgaa gggcccgaat gacgccctgc tgcggtggcc cttcaaccag 3601 aaggtgacct taatgctgct cgaccagaat aaccgggagc acgtgattga cgccttcagg 3661 cccgacgtga cttcatcctc ttttcagagg ccagtcaacg acatgaacat cgcaagcggc 3721 tgccccctct tctgccccgt ctccaagatg gaggcaaaga attcctacgt gcgggacgat 3781 gccatcttca tcaaggccat tgtggacctg acagggctcg GaGGTGGAGG TAGTGGAGGA 3841 GGCGGAtcAg gtggaggcgg atcaGAATTC gccaccatgg tgagcaaggg cgaggaggat 3901 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 3961 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4021 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4081 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4141 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4201 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4261 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4321 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4381 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4441 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4501 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4561 ggcggcatgg acgagctgta caagtagGGA TCCGCGGCCG CGAAGGATCT GCGATCGCTC 4621 CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG 4681 GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT 4741 CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT 4801 CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG 4861 GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT 4921 GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT 4981 AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG 5041 ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC 5101 GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGGCCACCA 5161 TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA 5221 ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC 5281 GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG 5341 TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA 5401 ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC 5461 ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC 5521 GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAGT CGACAATCAA CCTCTGGATT 5581 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 5641 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 5701 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 5761 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 5821 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 5881 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 5941 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT 6001 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 6061 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 6121 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGTAC CTTTAAGACC AATGACTTAC 6181 AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT 6241 CACTCCCAAC GAAAATAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG 6301 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC 6361 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA 6421 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT 6481 ATTATTCAGT ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT 6541 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA 6601 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC 6661 TGGCTCTAGC TATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT 6721 GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 6781 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG ACTTTTGCAG AGACGGCCCA AATTCGTAAT 6841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 6901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 6961 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 7021 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 7081 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 7141 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 7201 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 7261 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 7321 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 7381 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 7441 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 7501 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 7561 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 7621 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 7681 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 7741 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 7801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 7861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 7921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 7981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 8041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 8101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 8161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 8221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 8281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 8341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 8401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 8461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 8521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 8581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 8641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 8701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 8761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 8821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 8881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 8941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 9001 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT 9061 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 9121 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 9181 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 9241 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 9301 GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT 9361 CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC 9421 CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.