pCMV-EMC1-miniTurboID-Flag pla

pCMV-EMC1-miniTurboID-Flag plasmid

PVT97008

pCMV-EMC1-miniTurboID-Flag plasmid Information

Plasmid name: pCMV-EMC1(human)-miniTurboID-Flag
Alias: NM_015047.3;CAVIPMR; KIAA0090
Gene lenght: 2979bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-EMC1-miniTurboID-Flag plasmid Description

pCMV-EMC1-miniTurboID-Flag is a plasmid suitable for protein expression in mammalian cells.

pCMV-EMC1-miniTurboID-Flag plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTCTCG AGGCCACCat ggcggctgag tgggcttctc gtttctggct 961 ttgggctacg ctgctgattc ctgcggccgc ggtctacgaa gaccaagtgg gcaagtttga 1021 ttggagacag caatatgttg ggaaggtcaa gtttgcctcc ttggaatttt cccctggatc 1081 caagaagttg gttgtagcca cagagaagaa tgtgattgca gcattaaatt cccgaactgg 1141 ggagatcttg tggcgccatg ttgacaaggg cacggcagaa ggggctgtgg atgccatgct 1201 gctgcacgga caggatgtga tcactgtgtc caatggaggc cgaatcatgc gttcctggga 1261 gactaacatc gggggcctga actgggagat aaccctggac agtggcagtt tccaggcact 1321 tgggctggtt ggcctgcagg agtctgtaag gtacatcgca gtcctgaaga agactacact 1381 tgccctccat cacctctcca gtgggcacct caagtgggtg gaacatctcc cagaaagtga 1441 cagcatccac taccagatgg tgtattctta cggctctggg gtggtgtggg ccctcggagt 1501 tgttcccttc agccatgtga acattgtcaa gtttaatgtg gaagatggag agattgttca 1561 gcaggttagg gtttcaactc cgtggctgca gcacctgtct ggagcctgtg gtgtggtgga 1621 tgaggctgtc ctggtgtgtc ctgacccgag ctcacgttcc ctccaaactt tggctctgga 1681 gacggaatgg gagttgagac agatcccact gcagtctctc gacttagaat ttggaagtgg 1741 attccaaccc cgggtcctgc ctacccagcc caacccagtg gacgcttccc gggcccagtt 1801 cttcctgcac ttgtccccaa gccactatgc tctgctgcag taccattatg gaacgctgag 1861 tttgcttaaa aacttcccac agactgccct agtgagcttt gccaccactg gggagaagac 1921 ggtggctgca gtcatggcct gtcggaatga agtgcagaaa agtagcagtt ctgaagatgg 1981 gtcaatgggg agcttttcgg agaagtctag ttcaaaggac tctctggctt gcttcaatca 2041 gacctacacc attaacctat acctcgtgga gacaggtcgg cggctgctgg acaccacgat 2101 aacatttagc ctggaacaga gcggcactcg gcctgagcgg ctgtatatcc aggtgttctt 2161 gaagaaggat gactcagtgg gctaccgggc tttggtgcag acagaggatc atctgctact 2221 tttcctgcag cagttggcag ggaaggtggt gctgtggagc cgtgaggagt ccctggcaga 2281 agtggtgtgc ctagagatgg tggacctccc cctgactggg gcacaggccg agctggaagg 2341 agaatttggc aaaaaggcag atggcttgct ggggatgttc ctgaaacgcc tctcgtctca 2401 gcttatcctg ctgcaagcat ggacttccca cctctggaaa atgttttatg atgctcggaa 2461 gccccggagt cagattaaga atgagatcaa cattgacacc ctggccagag atgaattcaa 2521 cctccagaag atgatggtga tggtaacagc ctcaggcaag ctttttggca ttgagagcag 2581 ctctggcacc atcctgtgga aacagtatct acccaatgtc aagccagact cctcctttaa 2641 actgatggtc cagagaacta ctgctcattt cccccatccc ccacagtgca ccctgctggt 2701 gaaggacaag gagtcgggaa tgagttctct gtatgtcttc aatcccattt ttgggaagtg 2761 gagtcaggta gctcccccag tgctgaagcg ccccatcttg cagtccttgc ttctcccagt 2821 catggatcaa gactacgcca aggtgttgct gttgatagat gatgaataca aggtcacagc 2881 ttttccagcc actcggaatg tcttgcgaca gctacatgag cttgcccctt ccatcttctt 2941 ctatttggtg gatgcagagc agggacggct gtgtggatat cggcttcgaa aggatctcac 3001 cactgagctg agttgggagc tgaccattcc cccagaagta cagcggatcg tcaaggtgaa 3061 ggggaaacgc agcagtgagc acgttcattc ccagggccgt gtgatggggg accgcagtgt 3121 gctctacaag agcctgaacc ccaacctgct ggccgtggtg acagagagca cagacgcgca 3181 ccatgagcgc acctttattg gcatcttcct cattgatggc gtcactgggc gtatcattca 3241 ctcctctgtg cagaagaaag ccaaaggccc tgtccatatc gtgcattcag agaactgggt 3301 ggtgtaccag tactggaaca ccaaggctcg gcgcaacgag tttaccgtac tggagctcta 3361 tgagggcact gagcaataca acgccaccgc cttcagctcc ctggaccgcc cccagctgcc 3421 ccaggtcctc cagcagtcct atatcttccc gtcctccatc agtgccatgg aggccaccat 3481 caccgaacgg ggcatcacca gccgacacct gctgattgga ctaccttctg gagcaattct 3541 ttcccttcct aaggctttgc tggatccccg ccgccccgag atcccaacag aacaaagcag 3601 agaggagaac ttaatcccgt attctccaga tgtacagata cacgcagagc gattcatcaa 3661 ctataaccag acagtttctc gaatgcgagg tatctacaca gctccctcgg gtctggagtc 3721 cacttgtttg gttgtggcct atggtttgga catttaccaa actcgagtct acccatccaa 3781 gcagtttgac gttctgaagg atgactatga ctacgtgtta atcagcagcg tcctctttgg 3841 cctggttttt gccaccatga tcactaagag actggcacag gtgaagctcc tgaatcgggc 3901 ctggcgaGAA TTCggTggAg gtggaagcgg tggaggtggt tcaggcggtg gcGGTTCCGG 3961 AGGGGGGGGC GGATCAGGTG GAGGGGGCAG TGGAGGCGGA GGAAGTGGAG GGGGCGGAAG 4021 TGGAGGAGGG GGATCTGGCG GTGGAGGATC AGGCGGTGGC GGTTCTGGCG GGGGTGGATC 4081 TGGTGGCGGT GGAAGTGGCG GCGGTGGCAG CGGATCCATC CCCCTGCTGA ACGCCAAACA 4141 GATCCTGGGC CAGCTGGACG GCGGCTCCGT GGCTGTTCTG CCCGTGGTGG ATAGCACCAA 4201 CCAGTACCTG CTGGATAGAA TTGGCGAGCT GAAGAGCGGC GATGCCTGCA TCGCCGAGTA 4261 TCAGCAGGCC GGCAGGGGCA GCAGGGGCAG GAAATGGTTC AGCCCCTTCG GCGCCAACCT 4321 GTATCTGTCC ATGTTCTGGA GGCTGAAAAG GGGCCCTGCC GCCATCGGCC TGGGCCCTGT 4381 GATCGGAATC GTGATGGCCG AGGCCCTGAG AAAACTGGGC GCCGACAAGG TGAGGGTGAA 4441 ATGGCCCAAC GACCTGTACC TGCAGGATAG GAAGCTGGCC GGCATCCTGG TGGAGCTGGC 4501 CGGAATCACC GGCGACGCCG CTCAGATCGT GATCGGCGCC GGCATCAACG TGGCCATGAG 4561 GAGGGTGGAG GAGAGCGTGG TGAATCAGGG CTGGATCACC CTGCAGGAGG CCGGCATCAA 4621 TCTGGACAGA AATACCCTGG CCGCCATGCT GATCAGAGAG CTGAGGGCCG CCCTGGAGCT 4681 GTTCGAGCAG GAGGGCCTGG CCCCCTACCT GTCCAGGTGG GAGAAGCTGG ATAACTTCAT 4741 CAACAGGCCT GTGAAGCTGA TCATTGGCGA CAAGGAGATA TTCGGCATTA GCAGAGGCAT 4801 CGATAAACAG GGCGCCCTGC TGCTGGAGCA GGACGGAGTG ATCAAGCCCT GGATGGGCGG 4861 CGAGATCAGC CTGAGAAGCG CCGAGAAGGG CGGCGACTAC AAGGACCACG ACGGCGACTA 4921 CAAGGACCAC GACATCGACT ACAAGGACGA CGACGACAAG TAAGGGCCCG TTTAAACCCG 4981 CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 5041 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 5101 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG 5161 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC 5221 TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG 5281 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC 5341 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC 5401 CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 5461 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC 5521 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC 5581 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 5641 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG 5701 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 5761 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 5821 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 5881 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 5941 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG 6001 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 6061 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 6121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 6181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 6241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 6301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 6361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 6421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 6481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 6541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 6601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 6661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 6721 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 6781 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 6841 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 6901 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 6961 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 7021 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 7081 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 7141 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 7201 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 7261 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 7321 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 7381 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 7441 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 7501 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 7561 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 7621 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 7681 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 7741 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 7801 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 7861 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 7921 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 7981 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 8041 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 8101 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 8161 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 8221 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 8281 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 8341 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 8401 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 8461 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 8521 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 8581 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 8641 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 8701 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 8761 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 8821 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 8881 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 8941 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 9001 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 9061 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 9121 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 9181 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 9241 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 9301 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 9361 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.