pLV3-CMV-Ier3-3×FLAG-CopGFP-Pu

pLV3-CMV-Ier3-3×FLAG-CopGFP-Puro plasmid

PVT95072

pLV3-CMV-Ier3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Ier3(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_133662.2;cI-3; gly96; IEX-1
Gene lenght: 480bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ier3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Ier3-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Ier3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt gccactcgcg caaccatctc 2341 cacaccatga ctggcctgag ggccccttct ccagctccct ccaccggccc ggaactccgg 2401 cggggctctg gtcccgaaat tttcaccttc gaccctctcc cggagcgggc cgtggtgtcc 2461 accgcgcgtt tgaacacttc tcgcgggcac cgaaaacgca gccgaagggt gctctaccct 2521 cgagtggtcc ggcgccagct accaaccgag gaacccaaca ttgccaagag ggtcctcttt 2581 ctcctgttcg ccatcatctt ctgccagatt ttgatggctg aagagggtgt gtcgcagccc 2641 ctggctccgg aggatgctac cagcgccgtg acacctgagc ccatttctgc gcccattact 2701 gcgcccccgg tcctcgagcc tttgaacctg acctcggagt cctcggacta tgcgctggat 2761 cttaaagctt ttctccagca acatccggcg gccttcggat ccGACTACAA AGACCATGAC 2821 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGta gcggccgcGa 2881 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 2941 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 3001 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 3061 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 3121 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 3181 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 3241 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 3301 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 3361 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 3421 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 3481 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 3541 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 3601 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 3661 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 3721 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 3781 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 3841 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 3901 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 3961 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 4021 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 4081 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 4141 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 4201 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 4261 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 4321 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4381 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4441 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 4501 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 4561 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 4621 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 4681 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 4741 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 4801 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 4861 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4921 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4981 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5041 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5101 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5161 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5221 aattccgtgg tgttgtcggg gaaATCATCG TCCTTTCCTt ggctgctcgc ctgtgttgcc 5281 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5341 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctTcg ccttcgccct 5401 cagacgagtc ggatctccct ttggGccgcc tccccgcctg gtacctttaa gaccaatgac 5461 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 5521 aattcactcc caacgaaGat aagatctgct ttttgcttgt actgggtctc tctggttaga 5581 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 5641 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 5701 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 5761 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 5821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 5941 tgtctggctc tagctaTCCC GCCCCTAACT CCGCCCAtcc cgcccctaac tccgcccagt 6001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 6061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 6121 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 6181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 6241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 6301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 6361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 6421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6961 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 7021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 7261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 7321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 7381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 7441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 7501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 7561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 7621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 7681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 7741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 7801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 7861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 7921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 7981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 8221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 8281 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 8341 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 8401 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 8461 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 8521 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 8581 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 8641 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 8701 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 8761 tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.