pCMV-Peg10-HNRNPA1 plasmid

pCMV-Peg10-HNRNPA1 plasmid

PVT96990

pCMV-Peg10-HNRNPA1 plasmid Information

Plasmid name: pCMV-Peg10-HNRNPA1(human)
Alias: -
Gene lenght: 2591bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-Peg10-HNRNPA1 plasmid Description

pCMV-Peg10-HNRNPA1 is a plasmid suitable for protein expression in mammalian cells.

pCMV-Peg10-HNRNPA1 plasmid Sequence

1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat 241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt 301 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg 361 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag 421 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct 481 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc 541 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat 601 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg 661 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct 721 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta 781 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg 841 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgc tgggtcccga 901 ctgcccacct cctcctcctc cccctccccc caacaacaac aacaacaaca actccaagca 961 caccggccat aagagtgcgt gtgtccccaa catgaccgaa cgaagaaggg acgagctctc 1021 tgaagagatc aacaacttaa gagagaaggt catgaagcag tcggaggaga acaacaacct 1081 gcagagccag gtgcagaagc tcacagagga gaacaccacc cttcgagagc aagtggaacc 1141 cacccctgag gatgaggatg atgacatcga gctccgcggt gctgcagcag ctgctgcccc 1201 accccctcca atagaggaag agtgcccaga agacctccca gagaagttcg atggcaaccc 1261 agacatgctg gctcctttca tggcccagtg ccagatcttc atggaaaaga gcaccaggga 1321 tttctcagtt gatcgtgtcc gtgtctgctt cgtgacaagc atgatgaccg gccgtgctgc 1381 ccgttgggcc tcagcaaagc tggagcgctc ccactacctg atgcacaact acccagcttt 1441 catgatggaa atgaagcatg tctttgaaga ccctcagagg cgagaggttg ccaaacgcaa 1501 gatcagacgc ctgcgccaag gcatggggtc tgtcatcgac tactccaatg ctttccagat 1561 gattgcccag gacctggatt ggaacgagcc tgcgctgatt gaccagtacc acgagggcct 1621 cagcgaccac attcaggagg agctctccca cctcgaggtc gccaagtcgc tgtctgctct 1681 gattgggcag tgcattcaca ttgagagaag gctggccagg gctgctgcag ctcgcaagcc 1741 acgctcgcca ccccgggcgc tggtgttgcc tcacattgca agccaccacc aggtagatcc 1801 aaccgagccg gtgggaggtg cccgcatgcg cctgacgcag gaagaaaaag aaagacgcag 1861 aaagctgaac ctgtgcctct actgtggaac aggaggtcac tacgctgaca attgtcctgc 1921 caaggcctca aagtcttcgc cggcgggaaa ctccccggcc ccgctgtaga gggaccttca 1981 gcgaccgggc cagaaataat aaggtcccca caagatgatg cctcatctcc acacttgcaa 2041 gtgatgcttc agatacatct gccaggcagg cacacactct tcgtacgagc tatgattgat 2101 tcaggggcat ctggtaactt catagatcac gagtatgtag ctcagaacgg aattcctttg 2161 agaataaagg actggcccat cttggttgaa gctatcgatg ggaggcctat agcttccggt 2221 cccgtggtgc atgaaacaca tgatcttatt gtcgatcttg gggatcaccg cgaagtcctc 2281 tcatttgacg taacccagtc ccctttcttc cccgtggtcc tcggagtgcg gtggctgagt 2341 actcacgatc ctaatattac atggagcacc cggtcaatcg tatttgatag tgagtactgt 2401 aggtaccact gtcgaatgta tagtcctatt ccccctagtt tgcctccccc agctcctcaa 2461 ccacccctgt actatcccgt ggacggatat agggtgtacc agcccgttcg atattactac 2521 gtacaaaatg tttatacacc tgttgatgag cacgtctacc cagaccatag actcgtcgac 2581 cctcacatcg aaatgattcc aggagcccat agtatacctt cagggcacgt atactctctg 2641 tccgaacctg aaatggcagc tcttcgagat tttgtggcaa gaaatgtaaa agatgggcta 2701 attactccaa cgattgcacc taatggagcc caagttctcc aggtgaagag ggggtggaaa 2761 ctgcaagttt cttatgattg ccgagctcca aacaatttta ctatccagaa tcagtatcct 2821 cgcctatcta ttccaaattt agaagaccaa gcacacctgg caacgtacac tgaattcgta 2881 cctcaaatac ctggatacca aacatacccc acatatgccg cgtacccgac ctacccagta 2941 ggattcgcct ggtacccagt gggacgagac ggacaaggaa gatcactata tgtacctgtg 3001 atgatcactt ggaatccaca ctggtaccgc cagcctccgg taccacagta cccgccgcca 3061 cagccgccgc ctccaccacc accaccgccg ccgcctccat cttacagtac cctgCGTGGT 3121 GGCTTTGGTG GCAGCCGTGG TGGTGGTGGA TATGGTGGCA GTGGGGATGG CTATAATGGA 3181 TTTGGTAATG ATGGTGGTTA TGGAGGAGGC GGCCCTGGTT ACTCTGGAGG AAGCAGAGGC 3241 TATGGAAGTG GTGGACAGGG TTATGGAAAC CAGGGCAGTG GCTATGGCGG GAGTGGCAGC 3301 TATGACAGCT ATAACAACGG AGGCGGAGGC GGCTTTGGCG GTGGTAGTGG AAGCAATTTT 3361 GGAGGTGGTG GAAGCTACAA TGATTTTGGG AATTACAACA ATCAGTCTTC AAATTTTGGA 3421 CCCATGAAGG GAGGAAATTT TGGAGGCtat ccgtatgatg ttccggatta tgcatgagaa 3481 ttcaccccac cagtgcaggc tgcctatcag aaagtggtgg ctggtgtggc taatgccctg 3541 gcccacaagt atcactaagc tcgctttctt gctgtccaat ttctattaaa ggttcctttg 3601 ttccctaagt ccaactacta aactggggga tattatgaag ggccttgagc atctggattc 3661 tgcctaataa aaaacattta ttttcattgc aatgatgtat ttaaattatt tctgaatatt 3721 ttactaaaaa gggaatgtgg gaggtcagtg catttaaaac ataaagaaat gaagagctag 3781 ttcaaacctt gggaaaatac actatatctt aaactccatg aaagaaggtg aggctgcaaa 3841 cagctaatgc acattggcaa cagcccctga tgcctatgcc ttattcatcc ctcagaaaag 3901 gattcaagta gaggcttgat ttggaggtta aagttttgct atgctgtatt ttacattact 3961 tattgtttta gctgtcctca tgaatgtctt ttcactaccc atttgcttat cctgcatctc 4021 tcagccttga ctccactcag ttctcttgct tagagatacc acctttcccc tgaagtgttc 4081 cttccatgtt ttacggcgag atggtttctc ctcgcctggc cactcagcct tagttgtctc 4141 tgttgtctta tagaggtcta cttgaagaag gaaaaacagg gggcatggtt tgactgtcct 4201 gtgagccctt cttccctgcc tcccccactc acagtgaccc ggaatccctc gacatggcag 4261 tctagcacta gtgcggccgc agatctgctt cctcgctcac tgactcgctg cgctcggtcg 4321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4861 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 4921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5041 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5161 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5221 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5281 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5341 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5401 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 5461 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5521 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5581 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5641 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5701 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5761 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5821 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5881 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5941 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 6001 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 6061 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 6121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 6181 ttccgcgcac atttccccga aaagtgccac ctgacgtgga tcccctgagg gggcccccat 6241 gggctagagg atccggcctc ggcctctgca taaataaaaa aaattagtca gccatgagct 6301 tggcccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc 6361 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 6421 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 6481 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 6541 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 6601 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 6661 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 6721 gcagtacatc tacgtattag tcatcgctat taccatg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.