pCMV3-FAM171A2-HA-Hyg plasmid

pCMV3-FAM171A2-HA-Hyg plasmid

PVT96969

pCMV3-FAM171A2-HA-Hyg plasmid Information

Plasmid name: pCMV3-FAM171A2(human)-HA-Hyg
Alias: NM_198475.3
Gene lenght: 2478bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV3-FAM171A2-HA-Hyg plasmid Description

pCMV3-FAM171A2-HA-Hyg is a plasmid suitable for protein expression in mammalian cells.

pCMV3-FAM171A2-HA-Hyg plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG AGTACATTTA TATTGGCTCA TGTCCAATAT 241 GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT 301 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG 361 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA 421 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT 481 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA 541 AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT 601 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG 661 GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG 721 GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC 781 CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT 841 TAGTGAACCG TCAGATCCTC ACTCTCTTCC GCATCGCTGT CTGCGAGGGC CAGCTGTTGG 901 GCTCGCGGTT GAGGACAAAC TCTTCGCGGT CTTTCCAGTA CTCTTGGATC GGAAACCCGT 961 CGGCCTCCGA ACGGTACTCC GCCACCGAGG GACCTGAGCG AGTCCGCATC GACCGGATCG 1021 GAAAACCTCT CGAGAAAGGC GTCTAACCAG TCACAGTCGC AAGGTAGGCT GAGCACCGTG 1081 GCGGGCGGCA GCGGGTGGCG GTCGGGGTTG TTTCTGGCGG AGGTGCTGCT GATGATGTAA 1141 TTAAAGTAGG CGGTCTTGAG ACGGCGGATG GTCGAGGTGA GGTGTGGCAG GCTTGAGATC 1201 CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATT ACTTCTGCGC TAAGATTGTC 1261 AGTTTCCAAA AACGAGGAGG ATTTGATATT CACCTGGCCC GATCTGGCCA TACACTTGAG 1321 TGACAATGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGG TCCAAGTTTA 1381 AACTTTAATA CGACTCACTA TAGGGGCCGC CACCAAGCTT GGTACCGCTA GCGGATCCAT 1441 GCCGCCGGCG AGTGGCCCCA GCGTCCTCGC GCGGCTGTTG CCGCTGCTGG GGCTGCTGCT 1501 CGGCAGCGCC TCCCGGGCTC CCGGCAAGTC GCCGCCGGAG CCCCCCAGCC CGCAGGAGAT 1561 CCTGATCAAG GTGCAGGTGT ATGTGAGCGG GGAGCTGGTG CCCCTGGCCC GGGCCTCAGT 1621 GGATGTGTTT GGGAACCGGA CTCTGCTGGC AGCTGGCACC ACAGACTCAG AGGGTGTGGC 1681 CACCCTGCCC CTCAGTTATC GCTTGGGCAC CTGGGTGCTG GTCACTGCTG CCCGCCCTGG 1741 CTTCCTCACC AACTCTGTGC CCTGGCGTGT TGACAAGCTG CCCTTGTATG CGTCTGTCAG 1801 CCTCTACCTG CTCCCTGAGC GGCCGGCCAC GCTCATCCTC TATGAGGACC TGGTGCACAT 1861 TCTCCTAGGC TCTCCCGGTG CCCGCTCCCA GCCCTTGGTG CAGTTCCAGC GCCGGGCTGC 1921 CCGCCTGCCT GTCAGCTCCA CCTACAGCCA GCTCTGGGCG TCACTTACGC CTGCCAGCAC 1981 CCAGCAGGAA ATGCGGGCTT TCCCTGCCTT CCTGGGCACT GAGGCCTCCA GCTCAGGCAA 2041 TGGCTCCTGG CTGGAGCTGA TGCCCCTGAC TGCTGTGAGC GTGCACCTGC TGACAGGTAA 2101 TGGGACAGAG GTGCCGCTCT CAGGCCCCAT TCACCTGTCC CTGCCCGTGC CCTCCGAGAC 2161 TCGTGCCCTC ACCGTGGGCA CCAGCATTCC AGCCTGGAGA TTTGACCCCA AGAGTGGGCT 2221 GTGGGTGCGC AATGGCACTG GTGTAATCCG GAAGGAAGGC CGGCAGCTCT ACTGGACCTT 2281 CGTCTCCCCC CAGCTGGGGT ACTGGGTGGC CGCCATGGCC TCCCCCACGG CTGGGCTGGT 2341 CACCATCACG TCGGGCATCC AGGACATCGG CACCTACCAC ACCATCTTCT TGCTCACCAT 2401 CCTGGCAGCC CTGGCCCTGC TGGTGCTTAT CCTGCTGTGT CTGCTCATCT ACTACTGCCG 2461 GAGGCGCTGC CTGAAGCCGA GGCAACAGCA CCGCAAGCTG CAGCTCTCGG GGCCCTCTGA 2521 CGGTAACAAA CGAGACCAGG CCACCTCGAT GTCCCAGCTC CACCTCATCT GTGGGGGACC 2581 CCTGGAACCC GCCCCGTCGG GGGACCCCGA GGCTCCGCCT CCAGGCCCCC TCCACTCGGC 2641 CTTCTCCAGC TCCCGGGACT TGGCCTCCTC CCGGGATGAC TTCTTCCGCA CCAAGCCGCG 2701 CTCTGCCAGC CGCCCGGCCG CCGAGCCTTC GGGTGCCCGC GGGGGCGAGA GCGCCGGGCT 2761 CAAGGGCGCT CGCTCGGCCG AGGGCCCCGG CGGGCTGGAG CCCGGCCTAG AGGAGCACCG 2821 GCGGGGGCCC TCGGGGGCTG CGGCCTTCCT GCACGAGCCG CCCTCGCCGC CGCCGCCCTT 2881 CGACCACTAC CTGGGCCACA AGGGGGCGGC CGAGGGCAAG ACCCCCGACT TCCTGCTGTC 2941 GCAGTCGGTG GACCAGCTGG CGCGGCCGCC GTCGCTGGGC CAGGCGGGGC AGCTCATCTT 3001 CTGCGGCTCC ATCGACCACC TCAAGGACAA CGTCTACCGC AACGTCATGC CCACCCTGGT 3061 GATCCCCGCG CACTACGTGC GCCTCGGCGG CGAGGCGGGC GCCGCCGGCG TGGGCGACGA 3121 GCCGGCCCCG CCGGAGGGCA CGGCACCCGG CCCGGCGCGC GCTTTTCCCC AGCCCGACCC 3181 CCAGCGCCCG CAGATGCCGG GCCACTCGGG CCCGGGGGGC GAGGGCGGCG GGGGCGGCGG 3241 CGAGGGCTGG GGGGCCGGGC GCGCGGCGCC CGTCAGTGGC TCAGTCACCA TCCCTGTGCT 3301 ATTCAACGAG TCCACCATGG CGCAGCTCAA CGGGGAGCTG CAGGCCCTGA CCGAGAAGAA 3361 GCTGCTGGAA CTGGGCGTGA AGCCGCACCC GCGCGCCTGG TTCGTGTCCC TCGACGGGCG 3421 CTCCAACTCG CAAGTGCGCC ACTCTTACAT CGACCTGCAG GCGGGCGGCG GGGCACGCAG 3481 CACCGACGCC AGCCTGGACT CGGGCGTAGA TGTCCACGAG GCGCGGCCCG CGCGCCGCCG 3541 GCCCGCGAGG GAGGAGCGGG AGCGCGCCCC GCCTGCCGCG CCGCCGCCGC CGCCCGCGCC 3601 CCCGCGCCTG GCGCTCAGCG AGGACACGGA GCCCAGCAGC AGCGAGAGCC GCACGGGCCT 3661 CTGCTCTCCG GAGGACAACT CGCTGACGCC GCTGCTGGAC GAGGTGGCGG CGCCCGAGGG 3721 CCGGGCGGCC ACGGTACCCC GGGGGCGGGG CCGCAGCCGC GGGGACAGCT CCCGCAGCAG 3781 CGCCAGCGAG CTGCGGCGCG ACTCGCTCAC CAGCCCGGAG GACGAGCTGG GGGCGGAGGT 3841 GGGCGACGAG GCGGGAGACA AGAAGAGCCC GTGGCAGCGG CGGGAGGAGC GGCCGCTGAT 3901 GGTGTTCAAC GTCAAGCCCG GGGGTGGAGG CTCTTATCCT TACGACGTGC CTGACTACGC 3961 CTAAACTCGA GTCTAGAGCG GCCGCCGAAT TCGGGCCCGT TTAAACCCGC TGATCAGCCT 4021 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA 4081 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT 4141 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG 4201 ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG 4261 AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG 4321 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 4381 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC AGGCTTTCCC CGTCAAGCTC 4441 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 4501 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 4561 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 4621 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 4681 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG 4741 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 4801 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 4861 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 4921 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 4981 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 5041 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CTCGGGAGCT TGTATATCCA TTTTCGGATC 5101 TGATCAGCAC GTGATGAAAA AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT 5161 CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC 5221 TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG 5281 TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA 5341 AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA 5401 GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC 5461 GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT 5521 CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA 5581 TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA 5641 GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA 5701 CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA 5761 CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG 5821 GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT 5881 TGCAGGATCG CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA 5941 GAGCTTGGTT GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT 6001 CGTCCGATCC GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT 6061 CTGGACCGAT GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG 6121 TCCGAGGGCA AAGGAATAGC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA 6181 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCTGGCTGG ATGATCCTCC AGCGCGGGGA 6241 TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 6301 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 6361 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA 6421 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 6481 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 6541 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 6601 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 6661 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 6721 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 6781 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 6841 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 6901 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 6961 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 7021 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 7081 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 7141 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 7201 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 7261 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 7321 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 7381 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 7441 GATCTTTTCT ACGGGGTCTG AGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 7501 AAATATGTAT CCGCTCATGA ATTAATTCTT AGAAAAACTC ATCGAGCATC AAATGAAACT 7561 GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG 7621 AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA 7681 TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT 7741 CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGTTTATGCA 7801 TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT 7861 CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT 7921 TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT 7981 CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCAG 8041 GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG 8101 GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG 8161 CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC 8221 GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT 8281 CAGCATCCAT GTTGGAATTT AATCGCGGCC TAGAGCAAGA CGTTTCCCGT TGAATATGGC 8341 TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGACCAAA 8401 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAT CCGCGCACAT 8461 TTCCCCGAAA AGTGCCACCT GACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.