pCMV-EGFP-Foxp3-Myc-FLAG-Neo p

pCMV-EGFP-Foxp3-Myc-FLAG-Neo plasmid

PVT96965

pCMV-EGFP-Foxp3-Myc-FLAG-Neo plasmid Information

Plasmid name: pCMV-EGFP-Foxp3(mouse)-Myc-FLAG-Neo
Alias: NM_001199347.2;JM2; scurfin; sf
Gene lenght: 1287bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-Foxp3-Myc-FLAG-Neo plasmid Description

pCMV-EGFP-Foxp3-Myc-FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-Foxp3-Myc-FLAG-Neo plasmid Sequence

1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCgccacca tggtgagcaa 1021 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 1081 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 1141 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 1201 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 1261 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 1321 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 1381 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 1441 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 1501 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 1561 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 1621 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 1681 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggGaG GCGGAGGTAG 1741 TGGAGGAGGG GGAAGCATGC CCAACCCTAG GCCAGCCAAG CCTATGGCTC CTTCCTTGGC 1801 CCTTGGCCCA TCCCCAGGAG TCTTGCCAAG CTGGAAGACT GCACCCAAGG GCTCAGAACT 1861 TCTAGGGACC AGGGGCTCTG GGGGACCCTT CCAAGGTCGG GACCTGCGAA GTGGGGCCCA 1921 CACCTCTTCT TCCTTGAACC CCCTGCCACC ATCCCAGCTG CAGCTGCCTA CAGTGCCCCT 1981 AGTCATGGTG GCACCGTCTG GGGCCCGACT AGGTCCCTCA CCCCACCTAC AGGCCCTTCT 2041 CCAGGACAGA CCACACTTCA TGCATCAGCT CTCCACTGTG GATGCCCATG CCCAGACCCC 2101 TGTGCTCCAA GTGCGTCCAC TGGACAACCC AGCCATGATC AGCCTCCCAC CACCTTCTGC 2161 TGCCACTGGG GTCTTCTCCC TCAAGGCCCG GCCTGGCCTG CCACCTGGGA TCAATGTGGC 2221 CAGTCTGGAA TGGGTGTCCA GGGAGCCAGC TCTACTCTGC ACCTTCCCAC GCTCGGGTAC 2281 ACCCAGGAAA GACAGCAACC TTTTGGCTGC ACCCCAAGGA TCCTACCCAC TGCTGGCAAA 2341 TGGAGTCTGC AAGTGGCCTG GTTGTGAGAA GGTCTTCGAG GAGCCAGAAG AGTTTCTCAA 2401 GCACTGCCAA GCAGATCATC TCCTGGATGA GAAAGGCAAG GCCCAGTGCC TCCTCCAGAG 2461 AGAAGTGGTG CAGTCTCTGG AGCAGCAGCT GGAGCTGGAA AAGGAGAAGC TGGGAGCTAT 2521 GCAGGCCCAC CTGGCTGGGA AGATGGCGCT GGCCAAGGCT CCATCTGTGG CCTCAATGGA 2581 CAAGAGCTCT TGCTGCATCG TAGCCACCAG TACTCAGGGC AGTGTGCTCC CGGCCTGGTC 2641 TGCTCCTCGG GAGGCTCCAG ACGGCGGCCT GTTTGCAGTG CGGAGGCACC TCTGGGGAAG 2701 CCATGGCAAT AGTTCCTTCC CAGAGTTCTT CCACAACATG GACTACTTCA AGTACCACAA 2761 TATGCGACCC CCTTTCACCT ATGCCACCCT TATCCGATGG GCCATCCTGG AAGCCCCGGA 2821 GAGGCAGAGG ACACTCAATG AAATCTACCA TTGGTTTACT CGCATGTTCG CCTACTTCAG 2881 AAACCACCCC GCCACCTGGA AGAATGCCAT CCGCCACAAC CTGAGCCTGC ACAAGTGCTT 2941 TGTGCGAGTG GAGAGCGAGA AGGGAGCAGT GTGGACCGTA GATGAATTTG AGTTTCGCAA 3001 GAAGAGGAGC CAACGCCCCA ACAAGTGCTC CAATCCCTGC CCTACGCGTA CGCGGCCGCT 3061 CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA 3121 TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG 3181 GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG 3241 TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT 3301 TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA 3361 CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC 3421 TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT 3481 TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG 3541 GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT 3601 GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA 3661 TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG 3721 CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA 3781 CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA 3841 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 3901 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 3961 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 4021 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 4081 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 4141 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 4201 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 4261 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 4321 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 4381 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 4441 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 4501 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 4561 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 4621 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 4681 AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC 4741 CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT 4801 TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG 4861 CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA 4921 GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG 4981 GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA 5041 ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC 5101 AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT 5161 CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA 5221 GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA 5281 TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC 5341 CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC 5401 CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT 5461 CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA 5521 TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC 5581 GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT 5641 CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA 5701 GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT 5761 GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG 5821 CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC 5881 CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA 5941 TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA 6001 AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA 6061 ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC 6121 GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC 6181 ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA 6241 TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA 6301 CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA 6361 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 6421 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 6481 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 6541 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 6601 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 6661 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 6721 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 6781 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 6841 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.