pCMV-CP-Neo plasmid

pCMV-CP-Neo plasmid

PVT90202

pCMV-CP-Neo plasmid Information

Plasmid name: pCMV-CP(human)-Neo
Alias: NM_000096.4;AB073614; CP-2
Gene lenght: 3198bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-CP-Neo plasmid Description

pCMV-CP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CP-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg aagattttga tacttggtat ttttctgttt ttatgtagta ccccagcctg 961 ggcgaaagaa aagcattatt acattggaat tattgaaacg acttgggatt atgcctctga 1021 ccatggggaa aagaaactta tttctgttga cacggaacat tccaatatct atcttcaaaa 1081 tggcccagat agaattggga gactatataa gaaggccctt tatcttcagt acacagatga 1141 aacctttagg acaactatag aaaaaccggt ctggcttggg tttttaggcc ctattatcaa 1201 agctgaaact ggagataaag tttatgtaca cttaaaaaac cttgcctcta ggccctacac 1261 ctttcattca catggaataa cttactataa ggaacatgag ggggccatct accctgataa 1321 caccacagat tttcaaagag cagatgacaa agtatatcca ggagagcagt atacatacat 1381 gttgcttgcc actgaagaac aaagtcctgg ggaaggagat ggcaattgtg tgactaggat 1441 ttaccattcc cacattgatg ctccaaaaga tattgcctca ggactcatcg gacctttaat 1501 aatctgtaaa aaagattctc tagataaaga aaaagaaaaa catattgacc gagaatttgt 1561 ggtgatgttt tctgtggtgg atgaaaattt cagctggtac ctagaagaca acattaaaac 1621 ctactgctca gaaccagaga aagttgacaa agacaacgaa gacttccagg agagtaacag 1681 aatgtattct gtgaatggat acacttttgg aagtctccca ggactctcca tgtgtgctga 1741 agacagagta aaatggtacc tttttggtat gggtaatgaa gttgatgtgc acgcagcttt 1801 ctttcacggg caagcactga ctaacaagaa ctaccgtatt gacacaatca acctctttcc 1861 tgctaccctg tttgatgctt atatggtggc ccagaaccct ggagaatgga tgctcagctg 1921 tcagaatcta aaccatctga aagccggttt gcaagccttt ttccaggtcc aggagtgtaa 1981 caagtcttca tcaaaggata atatccgtgg gaagcatgtt agacactact acattgccgc 2041 tgaggaaatc atctggaact atgctccctc tggtatagac atcttcacta aagaaaactt 2101 aacagcacct ggaagtgact cagcggtgtt ttttgaacaa ggtaccacaa gaattggagg 2161 ctcttataaa aagctggttt atcgtgagta cacagatgcc tccttcacaa atcgaaagga 2221 gagaggccct gaagaagagc atcttggcat cctgggtcct gtcatttggg cagaggtggg 2281 agacaccatc agagtaacct tccataacaa aggagcatat cccctcagta ttgagccgat 2341 tggggtgaga ttcaataaga acaacgaggg cacatactat tccccaaatt acaaccccca 2401 gagcagaagt gtgcctcctt cagcctccca tgtggcaccc acagaaacat tcacctatga 2461 atggactgtc cccaaagaag taggacccac taatgcagat cctgtgtgtc tagctaagat 2521 gtattattct gctgtggaac ccactaaaga tatattcact gggcttattg ggccaatgaa 2581 aatatgcaag aaaggaagtt tacatgcaaa tgggagacag aaagatgtag acaaggaatt 2641 ctatttgttt cctacagtat ttgatgagaa tgagagttta ctcctggaag ataatattag 2701 aatgtttaca actgcacctg atcaggtgga taaggaagat gaagactttc aggaatctaa 2761 taaaatgcac tccatgaatg gattcatgta tgggaatcag ccgggtctca ctatgtgcaa 2821 aggagattcg gtcgtgtggt acttattcag cgccggaaat gaggccgatg tacatggaat 2881 atacttttca ggaaacacat atctgtggag aggagaacgg agagacacag caaacctctt 2941 ccctcaaaca agtcttacgc tccacatgtg gcctgacaca gaggggactt ttaatgttga 3001 atgccttaca actgatcatt acacaggcgg catgaagcaa aaatatactg tgaaccaatg 3061 caggcggcag tctgaggatt ccaccttcta cctgggagag aggacatact atatcgcagc 3121 agtggaggtg gaatgggatt attccccaca aagggagtgg gaaaaggagc tgcatcattt 3181 acaagagcag aatgtttcaa atgcattttt agataaggga gagttttaca taggctcaaa 3241 gtacaagaaa gttgtgtatc ggcagtatac tgatagcaca ttccgtgttc cagtggagag 3301 aaaagctgaa gaagaacatc tgggaattct aggtccacaa cttcatgcag atgttggaga 3361 caaagtcaaa attatcttta aaaacatggc cacaaggccc tactcaatac atgcccatgg 3421 ggtacaaaca gagagttcta cagttactcc aacattacca ggtgaaactc tcacttacgt 3481 atggaaaatc ccagaaagat ctggagctgg aacagaggat tctgcttgta ttccatgggc 3541 ttattattca actgtggatc aagttaagga cctctacagt ggattaattg gccccctgat 3601 tgtttgtcga agaccttact tgaaagtatt caatcccaga aggaaactgg aatttgccct 3661 tctgtttcta gtttttgatg agaatgaatc ttggtactta gatgacaaca tcaaaacata 3721 ctctgatcac cccgagaaag taaacaaaga tgatgaggaa ttcatagaaa gcaataaaat 3781 gcatgctatt aatggaagaa tgtttggaaa cctacaaggc ctcacaatgc acgtgggaga 3841 tgaagtcaac tggtatctga tgggaatggg caatgaaata gacttacaca ctgtacattt 3901 tcacggccat agcttccaat acaagcacag gggagtttat agttctgatg tctttgacat 3961 tttccctgga acataccaaa ccctagaaat gtttccaaga acacctggaa tttggttact 4021 ccactgccat gtgaccgacc acattcatgc tggaatggaa accacttaca ccgttctaca 4081 aaatgaagac accaaatctg gctgaCTCGA GTCTAGAGGG CCCGTTTAAA CCCGCTGATC 4141 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 4201 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 4261 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 4321 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA 4381 GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT 4441 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 4501 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 4561 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 4621 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 4681 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 4741 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 4801 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT 4861 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 4921 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 4981 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 5041 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 5101 TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 5161 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 5221 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 5281 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 5341 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 5401 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 5461 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 5521 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 5581 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 5641 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 5701 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 5761 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 5821 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 5881 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 5941 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 6001 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 6061 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 6121 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 6181 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 6241 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 6301 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 6361 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 6421 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 6481 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 6541 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 6601 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 6661 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 6721 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 6781 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 6841 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 6901 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 6961 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 7021 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 7081 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 7141 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 7201 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 7261 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 7321 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 7381 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 7441 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 7501 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 7561 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 7621 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 7681 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 7741 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 7801 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 7861 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 7921 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 7981 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 8041 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 8101 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 8161 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 8221 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 8281 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 8341 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 8401 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 8461 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 8521 CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG 8581 GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.