pLV3-CMV-LMX1B-3×FLAG-CopGFP-P

pLV3-CMV-LMX1B-3×FLAG-CopGFP-Puro plasmid

PVT96916

pLV3-CMV-LMX1B-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-LMX1B(human)-3×FLAG-CopGFP-Puro
Alias: NM_002316.4;FSGS10; LMX1.2; NPS1
Gene lenght: 1185bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LMX1B-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-LMX1B-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-LMX1B-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg atatagcaac aggtcccgag 2341 tcgctggaga ggtgcttccc tcgcgggcag acggactgcg ccaagatgtt ggacggcatc 2401 aagatggagg agcacgccct gcgccccggg cccgccactc tgggggtgct gctgggctcc 2461 gactgcccgc atcccgccgt ctgcgagggc tgccagcggc ccatctccga ccgcttcctg 2521 atgcgagtca acgagtcgtc ctggcacgag gagtgtttgc agtgcgcggc gtgtcagcaa 2581 gccctcacca ccagctgcta cttccgggat cggaaactgt actgcaaaca agactaccaa 2641 cagctcttcg cggccaagtg cagcggctgc atggagaaga tcgcccccac cgagttcgtg 2701 atgcgggcgc tggagtgcgt gtaccacctg ggctgcttct gctgctgcgt gtgtgaacgg 2761 cagctacgca agggcgacga attcgtgctc aaggagggcc agctgctgtg caagggtgac 2821 tacgagaagg agaaggacct gctcagctcc gtgagccccg acgagtccga ctccgtgaag 2881 agcgaggatg aagatgggga catgaagccg gccaaggggc agggcagtca gagcaagggc 2941 agcggggatg acgggaagga cccgcggagg cccaagcgac cccggaccat cctcaccacg 3001 cagcagcgaa gagccttcaa ggcctccttc gaggtctcgt cgaagccttg ccgaaaggtc 3061 cgagagacac tggcagctga gacgggcctc agtgtgcgcg tggtccaggt ctggtttcag 3121 aaccaaagag caaagatgaa gaagctggcg cggcggcacc agcagcagca ggagcagcag 3181 aactcccagc ggctgggcca ggaggtcctg tccagccgca tggagggcat gatggcttcc 3241 tacacgccgc tggccccacc acagcagcag atcgtggcca tggaacagag cccctacggc 3301 agcagcgacc ccttccagca gggcctcacg ccgccccaaa tgccagggaa cgactccatc 3361 ttccatgaca tcgacagcga tacctcctta accagcctca gcgactgctt cctcggctcc 3421 tcagacgtgg gctccctgca ggcccgcgtg gggaacccca tcgaccggct ctactccatg 3481 cagagttcct acttcgcctc cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3541 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 3601 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3661 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3721 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3781 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3841 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3901 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3961 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4021 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4081 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4141 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4201 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 4261 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4321 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4381 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4441 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4501 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4561 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 4621 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 4681 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 4741 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 4801 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 4861 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 4921 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 4981 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5041 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5101 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5161 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5221 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5281 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5341 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5401 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5461 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5521 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 5581 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5641 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5701 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5761 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5821 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5881 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5941 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 6001 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6061 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 6121 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6181 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6241 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6301 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6361 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6421 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6481 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6541 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6601 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6661 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 6721 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 6781 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 6841 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6901 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6961 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7021 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7081 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7141 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7201 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7261 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7321 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7381 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7441 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7501 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7561 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7621 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7681 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7741 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7801 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7861 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7921 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7981 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 8041 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8101 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8161 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8221 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8281 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8341 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8401 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8461 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8521 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8581 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8641 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 8701 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 8761 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8821 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8881 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8941 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 9001 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 9061 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 9121 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9181 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9241 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9301 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9361 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9421 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.