pLV3-CMV-HOXC13-3×FLAG-CopGFP-

pLV3-CMV-HOXC13-3×FLAG-CopGFP-Puro plasmid

PVT90313

pLV3-CMV-HOXC13-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HOXC13(human)-3×FLAG-CopGFP-Puro
Alias: NM_017410.3;ECTD9; HOX3; HOX3G
Gene lenght: 990bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HOXC13-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HOXC13-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HOXC13-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga cgacttcgct gctcctgcat 2341 ccacgctggc cggagagcct tatgtacgtc tatgaggaca gcgcggcgga gagcggcatc 2401 ggcggcggcg gcggaggagg aggcggcggc acgggcggag cggggggtgg ctgcagcgga 2461 gcgagccccg gcaaagcccc gagcatggat ggtctgggca gcagctgccc ggccagccac 2521 tgccgcgacc tgcttccgca ccccgtgctg ggccgcccgc cggctcccct gggcgcccct 2581 cagggcgccg tctatacgga catcccggcc ccggaggcgg cgcgccagtg tgccccgccg 2641 cccgcacccc ccacctcgtc cagcgccacc ctgggctacg gctacccctt cgggggcagc 2701 tactacggct gccgcctgtc gcacaacgtg aacctgcagc agaagccttg cgcctaccac 2761 ccgggcgata aatacccgga gccgtcgggc gccctgcccg gtgacgacct gtcctctagg 2821 gccaaggagt tcgccttcta ccccagcttc gccagctcct accaggcgat gcccggctac 2881 ctggacgtgt cggtggtgcc cgggatcagc gggcacccgg agccgcgtca cgacgccctc 2941 atccccgtcg aaggctacca gcactgggct ctctccaatg gctgggacag tcaggtgtac 3001 tgctccaagg agcagtcgca gtccgcccac ctctggaagt ctcccttccc agacgtggtt 3061 cccctgcagc ccgaggtgag cagctaccgg cgcgggcgca agaaacgcgt gccctacact 3121 aaggtgcagc tgaaggagct agagaaggaa tacgcggcta gcaagttcat caccaaagag 3181 aagcgccggc gcatctccgc caccacgaac ctctctgagc gccaggtaac catctggttc 3241 cagaaccggc gggtcaaaga gaagaaggtg gtcagcaaat cgaaagcgcc tcatctccac 3301 tccaccggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3361 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3421 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3481 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3541 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3601 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3661 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3721 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3781 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3841 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 3901 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 3961 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4021 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4081 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4141 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4201 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4261 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4321 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4381 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4441 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4501 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4561 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4621 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4681 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4741 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4801 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4861 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4921 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4981 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5041 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5101 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5161 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5221 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5281 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5341 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5401 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5461 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5521 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5581 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5641 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5701 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 5761 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5821 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5881 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 5941 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6001 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6061 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6121 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6181 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6241 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6301 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6361 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6421 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6481 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6541 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6601 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 6661 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6721 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6781 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6841 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6901 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6961 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7021 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7081 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7141 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7201 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7261 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7381 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7441 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7501 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7561 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7621 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7681 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7741 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7801 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7861 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7921 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7981 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8041 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8101 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8161 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8221 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8281 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8341 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8401 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8461 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8521 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8581 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8641 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8701 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8761 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8821 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 8881 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 8941 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9001 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9061 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9121 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9181 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9241 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.