pLV3-CMV-ERBB-3×FLAG-CopGFP-Pu

pLV3-CMV-ERBB-3×FLAG-CopGFP-Puro plasmid

PVT96901

pLV3-CMV-ERBB-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ERBB(Macaca)-3×FLAG-CopGFP-Puro
Alias: XM_01515940.2
Gene lenght: 4026bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: macaque
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ERBB-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ERBB-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ERBB-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga gggcgaacgg cgctctgcag 2341 gtgctgggct tgcttttcaa cctggcccgg ggctccgagg tgggcaactc tcaggcagtg 2401 tgtcctggga ctctgaatgg cctgagtgtg accggcgatg ctgagaacca ataccagaca 2461 ctgtacaagc tctacgagag gtgtgaggtg gtgatgggga acctcgagat tgtgctcacg 2521 ggacacaatg ctgacctctc cttcctgcag tggattcgag aagtgacagg ctatgtcctc 2581 gtggccatga atgagttctc tactctacca ttgcccaacc tccgagtggt gcgagggacc 2641 caggtctacg atgggaagtt tgccatcttc gtcatgttga actataacac caactccagc 2701 cacgctctgc gccagctccg cttgactcag ctcaccgaga ttctgtcagg gggtgtttat 2761 attgagaaga atgataagct ttgtcacatg gacacaattg actggaagga catcgtgagg 2821 gaccaagacg ctgagatagt ggtgaaggac aatggcagaa gctgtcccct ctgtcatgag 2881 gtttgcaagg ggcgatgctg gggtcctgga ccagaagact gccaaacatt gaccaagacc 2941 atctgtgctc ctcagtgtaa tggtcactgc tttgggccca accccaacca gtgctgccat 3001 gatgagtgtg ccgggggctg ctcaggccct caggacacag actgctttgc ctgccggcac 3061 ttcaatgaca gtggagcctg tgtacctcgc tgtccacagc ctcttgtcta caacaagcta 3121 actttccagc tggaacccaa tccccacacc aagtatcagt atggaggagt ttgtgtagcc 3181 agctgtcccc ataactttgt ggtggatcaa acatcctgtg ttagggcctg tcctcctgac 3241 aagatggaag tagataaaaa tggactcaag atgtgtgagc cttgtggggg actatgtccc 3301 aaagcctgtg agggcacagg ctctgggagc cgcttccaga ctgtggactc gagcaacatc 3361 gatggatttg tgaactgcac caagatcttg ggcaacctgg actttctgat caccggcctc 3421 aatggagacc cctggcacaa gatccctgcc ctggacccag agaagctcaa tgtcttccgg 3481 acagtacggg agatcacagg ttacctgaac atccagtcct ggccacccca catgtacaac 3541 ttcagtgttt tttccaactt gacaaccatt ggaggcagaa gcctctacaa ccggggcttc 3601 tcattgttga tcatgaagaa cttgaatgtc acatctctgg gcttccgatc cctgaaggaa 3661 attagtgctg ggcgtatcta tataagtgcc aataggcagc tctgctacca ccactctttg 3721 aactggacca aggtgcttcg ggggcctaca gaagagcgac tagacatcaa gcataatcgt 3781 ccgcgtagag actgcgtggc agagggcaaa gtgtgtgacc cactgtgctc ctctggggga 3841 tgctggggcc caggccctgg tcagtgcttg tcctgtcgaa actacagccg aggaggtgtc 3901 tgtgtgaccc actgcaactt tctgaatggg gagcctcgag agtttgccca tgaggctgaa 3961 tgcttctcct gccacccgga atgccaaccc atggagggca ctgccacatg caatggctcg 4021 ggctctgata cttgtgctca atgtgcccat tttcgagatg ggccccactg tgtgagcagc 4081 tgcccccatg gagtcctagg tgccaagggc cccatctaca agtacccaga tgttcagaat 4141 gaatgtcggc cctgccatga gaactgcacc caggggtgta aaggaccaga gcttcaagac 4201 tgtttaggac aaacactggt gctgattggc aaaacccatc tgacaatggc tttgacagtg 4261 atagcaggat tggtagtgat tttcatgatg ctgggcggca cttttctcta ctggcgtggg 4321 cgccggattc agaataaaag ggctatgagg cgatacttgg aacggggtga gagcatagag 4381 cctctggacc ccagtgagaa ggctaacaaa gtcttggcca gaatcttcaa agagacagag 4441 ttaaggaagc ttaaagtgct tggctcgggt gtctttggaa ctgtgcacaa aggagtgtgg 4501 atccctgagg gtgaatcaat caagattcca gtctgcatta aaatcattga ggacaagagt 4561 ggacggcaaa gttttcaagc tgtgacagat catatgctgg ccattggcag cctggaccat 4621 gcccacattg tgcggctgct gggactatgc ccagggtcat ctctgcagct tgtcactcag 4681 tacttgcctc tgggttctct gctggatcat gtgagacaac accggggggc actggggcca 4741 cagctgctgc tcaactgggg agtacaaatt gccaagggta tgtactacct tgaagaacat 4801 ggtatggtgc atagaaacct ggctgcccga aatgtgctac tcaagtcacc cagtcaggtt 4861 caggtggcag attttggtgt ggctgacctg ctgcctcctg atgataagca gctgctatac 4921 agtgaggcca agactccaat taagtggatg gccctcgaga gtatccactt tgggaaatac 4981 acacaccaga gtgatgtctg gagctatggt gtgacagttt gggagttgat gacctttggg 5041 gcagagccct atgcagggct gcgactggct gaagtaccag acctgctaga gaagggggag 5101 cggttggcac agccccagat ctgcacaatt gatgtctaca tggtgatggt caagtgttgg 5161 atgattgatg agaacattcg cccaaccttt aaagaactag ccaatgagtt caccaggatg 5221 gcccgagacc caccacggta tctggtcata aagagagaga gtgggcctgg aatagcccct 5281 gggccagagc cccatggtct gacaaacaag aagctagagg aagtagagct ggagccagaa 5341 ctagacctag acttagactt ggaagcagag gaggacaacc tggcaaccac cacactgggc 5401 tctgccctca gcctaccagt tggaacactt aatcggccac gtgggagcca gagcctttta 5461 agtccatcat ctggatacat gcccatgaac cagggtaatc ttggggaggc ttgccaggag 5521 tctgcagttt ctgggagcag tgaatggtgc ccccgtccag tctctctaca cccaatgcca 5581 cggggatgcc tggcatcaga gtcatcagag ggccatgtaa caggctctga ggctgagctc 5641 caggagaaag tgtcaacgtg taggagccgg agcaggagcc ggagcccacg gccacgcgga 5701 gatagcgcct accattccca gcgccacagt ctgcttactc ctgttacccc actctcccca 5761 ccagggttag aggaagagga tgtcaacggt tatgtcatgc cagatacaca cctcaaaggt 5821 actccctcct cccgggaagg caccctttct tcagtgggtc tcagttctgt cctgggtact 5881 gaagaagaag atgaagatga ggagtatgaa tacatgaacc ggaggagaag gcacagtcca 5941 cctcgtcccc ctaggccaag ttcccttgag gagctgggtt atgagtacat ggatgtgggg 6001 tcagacctca gtgcttctct gggcagcaca cagagttgcc cactccaccc tgtaccagtc 6061 atgcccactg ctggcacaac tccagatgaa gactatgaat atatgaatcg gcaacgaggt 6121 ggaagtggtc ctgggggtga ttatgcagcc atgggggcct gcccagcatc tgagcaaggg 6181 tatgaagaga tgagagcttt tcaggggcct ggacatcagg ccccccatgt ccattacgcc 6241 cacctaaaaa ctctacgtag cttagaggct acagactctg cctttgataa ccctgattac 6301 tggcatagca ggcttttccc caaggctaat gcccagagaa cgggatccGA CTACAAAGAC 6361 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 6421 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 6481 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 6541 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 6601 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 6661 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 6721 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 6781 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 6841 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 6901 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 6961 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 7021 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 7081 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 7141 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 7201 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 7261 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 7321 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 7381 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 7441 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 7501 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 7561 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 7621 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 7681 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 7741 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 7801 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 7861 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 7921 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 7981 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 8041 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 8101 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 8161 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 8221 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 8281 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 8341 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 8401 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 8461 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 8521 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 8581 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 8641 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 8701 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 8761 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 8821 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 8881 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 8941 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 9001 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 9061 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 9121 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 9181 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 9241 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 9301 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 9361 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 9421 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 9481 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 9541 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 9601 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 9661 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 9721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 9781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 9841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 9901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 9961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 10021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 10081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 10141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 10201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 10261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 10321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 10381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 10441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 10501 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 10561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 10621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 10681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 10741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 10801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 10861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 10921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 10981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 11041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 11101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 11161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 11221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 11281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 11341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 11401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 11461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 11521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 11581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 11641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 11701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 11761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 11821 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 11881 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 11941 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 12001 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 12061 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 12121 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 12181 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 12241 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 12301 ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.