pLV3-CMV-PAN2-3×FLAG-CopGFP-Pu

pLV3-CMV-PAN2-3×FLAG-CopGFP-Puro plasmid

PVT96894

pLV3-CMV-PAN2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PAN2(human)-3×FLAG-CopGFP-Puro
Alias: NM_001127460.4;USP52
Gene lenght: 3606bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PAN2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PAN2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PAN2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga actttgaggg tctggaccct 2341 ggactggcag aatatgcccc agccatgcat tctgccctgg accctgtctt ggatgcccac 2401 ctgaacccaa gtctgctaca gaatgtggag ctggacccag agggagtggc cttggaggct 2461 cttcccgtcc aggaatcagt gcacataatg gaaggtgtct actctgaatt gcacagcgtg 2521 gtggctgaag tgggtgtacc tgtttccgtc tcccactttg acttgcacga ggagatgctg 2581 tgggtgggga gccacggggg ccatgccact tcattttttg gcccagcctt ggagcgctac 2641 tcatcctttc aagtcaatgg cagtgatgat attcggcaga tccagagcct ggagaatggt 2701 atcctttttc tcaccaagaa caacctcaag tatatggccc gtgggggcct cattatattt 2761 gattacctgc tggatgagaa tgaggatatg cacagtctcc tactgactga cagcagcact 2821 ctactcgttg gtgggctgca gaatcacata atagagattg atcttaacac tgtccaggag 2881 actcagaagt atgcagtaga gacgcctgga gtcaccatca tgagacagac aaatcgcttc 2941 ttcttctgcg gccacacgtc tggcaaggtt tccctgagag acctccgtac ttttaaggtg 3001 gaacatgagt ttgatgcctt ctcaggaagt ctgtcagact ttgatgtgca tggcaacctg 3061 ctagctgcct gtggcttctc cagccgcctc actggcctgg cctgcgaccg tttcctcaag 3121 gtgtatgatt tgcgcatgat gcgtgccatc acaccacttc aagtacatgt ggatcctgcc 3181 ttcttgcgct tcattcctac atatacttct cgtcttgcta tcatctctca gtcagggcag 3241 tgccaattct gtgaacccac aggcctggcc aacccagccg atatctttca tgtgaatcct 3301 gtggggcctc tgctaatgac atttgatgtg tcagccagca agcaggctct ggcctttggg 3361 gattctgagg gctgtgtgca cctctggact gattccccgg agccttcctt caacccctac 3421 tcccgtgaga ctgagtttgc tttgccgtgt ctcgtggact cactgcctcc tctggactgg 3481 agccaggacc tgctgcctct ttccctcatc cctgtcccac tcaccactga cacacttctc 3541 tctgattggc ctgctgccaa ctctgctcca gctcccaggc gagcaccacc cgtggatgca 3601 gagattctgc gcaccatgaa gaaggtgggc ttcattggct atgcgcccaa tccccgcacc 3661 aggctgcgca atcagatacc ctacagactc aaggagtcag acagtgaatt tgacagcttc 3721 agccaggtca ctgagtcacc agtaggacga gaagaggaac cacatctcca catggtttct 3781 aagaaatacc gcaaggtgac catcaaatat tccaagctag ggctggagga ctttgacttc 3841 aaacactaca ataagacctt gtttgctgga ttagagcccc acattcccaa cgcctactgt 3901 aactgcatga tccaggtgct ctatttcctg gagcctgtac gctgtctaat tcaaaaccac 3961 ctttgccaga aggagttctg tctggcatgt gagctgggct tcctgtttca catgttggac 4021 ctctctcgtg gtgacccttg ccagggcaat aattttcttc gggcattccg tactattcct 4081 gaggcctcag ccctcggtct aatcctggct gactcagatg aggcctcagg caagggcaat 4141 ctggccaggc tcattcagag gtggaatcgc ttcattctca ctcaactgca tcaagatatg 4201 caggagctgg aaataccaca ggcttatcga ggtgctggag gcagcagctt ttgctcatcg 4261 ggggactctg ttattgggca gctcttcagc tgtgagatgg agaactgcag cctctgccgc 4321 tgtggcagtg agaccgtgcg agcctcatcc actctgcttt tcacactctc ctaccctgat 4381 ggtagcaaaa gtgataaaac tgggaagaac tatgactttg ctcaggtgct gaagcgaagc 4441 atctgcctgg accagaatac acaggcctgg tgtgacacct gtgaaaagta ccagcccacg 4501 attcagaccc gcaacatccg ccatctgcca gatattcttg tcatcaattg tgaggtgaac 4561 agctcaaaag aggctgattt ctggagaatg caggctgagg ttgccttcaa gatggcagta 4621 aagaaacacg gtggggaaat ctccaagaac aaggaatttg ctttggctga ttggaaggaa 4681 ctagggagtc cagagggtgt gctggtgtgt ccctccattg aggagttgaa gaacgtctgg 4741 cttcctttct ccattcgcat gaagatgacc aaaaacaaag ggctggatgt ttgcaattgg 4801 actgatgggg atgagatgca gtggggccca gccagggcag aggaggagca tggtgtctat 4861 gtgtatgacc tgatggctac tgtggtacac atcctggact cacgcacagg gggcagcctg 4921 gtggctcaca tcaaagttgg agagacctac caccagcgca aggagggcgt tactcaccag 4981 cagtggtatc tgttcaatga ctttcttatt gaacctattg ataagcatga agctgtgcag 5041 tttgacatga attggaaagt acctgcaatc ctttattatg tcaaacggaa tctcaattcc 5101 agatacaacc tgaacatcaa gaaccctatt gaggcaagtg tcttgctggc tgaagcctcg 5161 ctggcacgga agcagcggaa aacacatact acctttattc cactgatgct gaatgagatg 5221 ccacagattg gggacctggt gggtctggat gctgagtttg tcacccttaa tgaggaggaa 5281 gcagagttac gcagtgatgg taccaagtct accattaaac caagccagat gtcagtagcc 5341 aggattacct gtgttcgggg ccagggaccc aatgagggta tccccttcat tgatgactac 5401 atctctaccc aggagcaggt ggtggattac ttgactcaat actcgggtat aaagcctggt 5461 gacctcgatg ccaaaatttc ctccaagcac ctaacaactc tcaagtctac ctacttaaag 5521 cttcgttttc tcattgacat tggagtcaag tttgtgggtc atggcctgca gaaggacttc 5581 cgggtcatca acctgatggt gcccaaggac caagtccttg acactgtcta cctgttccat 5641 atgccccgaa aacgaatgat ttccctgcga ttccttgctt ggtactttct ggacctgaag 5701 attcaagggg aaacccatga cagtattgag gatgcccgca cagcccttca gctgtaccga 5761 aagtatctgg agctaagcaa aaatggcact gagcctgagt ctttccacaa ggtgctcaag 5821 ggtctttatg agaagggcag aaagatggac tggaaggtgc ctgagcctga gggccaaaca 5881 agtcccaaga atgcagctgt cttctcctca gtgctggcgc tcggatccGA CTACAAAGAC 5941 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 6001 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 6061 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 6121 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 6181 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 6241 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 6301 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 6361 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 6421 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 6481 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 6541 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 6601 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 6661 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 6721 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 6781 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 6841 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 6901 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 6961 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 7021 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 7081 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 7141 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 7201 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 7261 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 7321 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 7381 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 7441 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 7501 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 7561 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 7621 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 7681 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 7741 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 7801 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 7861 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 7921 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 7981 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 8041 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 8101 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 8161 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 8221 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 8281 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 8341 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 8401 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 8461 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 8521 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 8581 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 8641 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 8701 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 8761 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 8821 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 8881 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 8941 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 9001 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 9061 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 9121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 9181 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 9241 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 9301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 9361 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 9421 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 9481 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 9541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 9601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 9661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 9721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 9781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 9841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 9901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 9961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 10021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 10081 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 10141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 10201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 10261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 10321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 10381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 10441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 10501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 10561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 10621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 10681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 10741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 10801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 10861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 10921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 10981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 11041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 11101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 11161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 11221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 11281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 11341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 11401 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 11461 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 11521 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 11581 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 11641 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 11701 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 11761 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 11821 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 11881 ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.