pLV3-CMV-Dnajb6-2-3×FLAG-CopGF

pLV3-CMV-Dnajb6-2-3×FLAG-CopGFP-Puro plasmid

PVT96892

pLV3-CMV-Dnajb6-2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Dnajb6(mouse)-2-3×FLAG-CopGFP-Puro
Alias: NM_001037941.3;HSJ-2; mDj4; Mrj
Gene lenght: 783bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Dnajb6-2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Dnajb6-2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Dnajb6-2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg tggattacta tgaagtgctg 2341 ggcgtgcaga gacatgcctc acctgaggac attaaaaagg cgtatcgaaa acaggcactt 2401 aaatggcacc cggacaaaaa tcctgaaaat aaagaagaag cagagcggaa gttcaaacaa 2461 gtagctgagg catatgaagt gttatcggat gctaaaaagc gggacatcta cgacaaatat 2521 ggcaaagaag gattaaatgg tggtggagga ggaggtggaa ttcattttga cagtccattt 2581 gagtttggct tcacattccg gaacccagat gatgtcttca gggaattttt tggtggaagg 2641 gacccatttt catttgactt ctttggtctt ctaggggact tccattttca ggcagtaagg 2701 attttcgcac aggaaaagaa gttgctccat ggctactacg tgtatgaggt gaccgtgcag 2761 cctactggtg gattcgagga agtggccagt gaggacttgg aacctagtga agaactagac 2821 cagccctgtt ttgaggagtc agtagaagac gccctgagtg acgagcacag tgaggttttg 2881 gatgtgatat ccagtgagga gctggacctt agtgaagatg agtcaagtga aggctgtagc 2941 tggggccagg gcggacttct cagtgaggac ctggacctcg tctccagcga ggaggaagcc 3001 agtctcgggg atgcggagga gctcagcgag gagtatgagg agctgctgag cgaggaggag 3061 cacagtgggc cggaccccga ggcgctgctt gttcaacaag gatccGACTA CAAAGACCAT 3121 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 3181 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3241 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 3301 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 3361 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 3421 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 3481 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 3541 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 3601 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 3661 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 3721 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 3781 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 3841 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 3901 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 3961 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 4021 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 4081 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 4141 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 4201 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 4261 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 4321 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 4381 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 4441 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 4501 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 4561 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 4621 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4681 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4741 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 4801 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 4861 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4921 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4981 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 5041 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 5101 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 5161 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5221 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5281 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5341 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5401 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5461 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5521 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 5581 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5641 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 5701 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 5761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 5821 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 5881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 5941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6241 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 6301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6421 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 6481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 8521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 8581 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 8641 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8701 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8761 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8821 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8881 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8941 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 9001 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 9061 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.