pLV3-CMV-SLC14A1-3×FLAG-CopGFP

pLV3-CMV-SLC14A1-3×FLAG-CopGFP-Puro plasmid

PVT96866

pLV3-CMV-SLC14A1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SLC14A1(human)-3×FLAG-CopGFP-Puro
Alias: NM_015865.7;HsT1341; HUT11; HUT11A; JK; Jk(a); Jk(b);RACH1; RACH2; UT-B1; UT1; UTE
Gene lenght: 1167bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLC14A1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SLC14A1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SLC14A1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aggacagccc cactatggtt 2341 agagtggaca gccccactat ggttaggggt gaaaaccagg tttcgccatg tcaagggaga 2401 aggtgcttcc ccaaagctct tggctatgtc accggtgaca tgaaagaact tgccaaccag 2461 cttaaagaca aacccgtggt gctccagttc attgactgga ttctccgggg catatcccaa 2521 gtggtgttcg tcaacaaccc cgtcagtgga atcctgattc tggtaggact tcttgttcag 2581 aacccctggt gggctctcac tggctggctg ggaacagtgg tctccactct gatggccctc 2641 ttgctcagcc aggacaggtc attaatagca tctgggctct atggctacaa tgccaccctg 2701 gtgggagtac tcatggctgt cttttcggac aagggagact atttctggtg gctgttactc 2761 cctgtatgtg ctatgtccat gacttgccca attttctcaa gtgcattgaa ttccatgctc 2821 agcaaatggg acctccccgt cttcaccctc cctttcaaca tggcgttgtc aatgtacctt 2881 tcagccacag gacattacaa tccattcttt ccagccaaac tggtcatacc tataactaca 2941 gctccaaata tctcctggtc tgacctcagt gccctggagt tgttgaaatc tataccagtg 3001 ggagttggtc agatctatgg ctgtgataat ccatggacag ggggcatttt cctgggagcc 3061 atcctactct cctccccact catgtgcctg catgctgcca taggatcatt gctgggcata 3121 gcagcgggac tcagtctttc agccccattt gaggacatct actttggact ctggggtttc 3181 aacagctctc tggcctgcat tgcaatggga ggaatgttca tggcgctcac ctggcaaacc 3241 cacctcctgg ctcttggctg tgccctgttc acggcctatc ttggagtcgg catggcaaac 3301 tttatggctg aggttggatt gccagcttgt acctggccct tctgtttggc cacgctattg 3361 ttcctcatca tgaccacaaa aaattccaac atctacaaga tgcccctcag taaagttact 3421 tatcctgaag aaaaccgcat cttctacctg caagccaaga aaagaatggt ggaaagccct 3481 ttgggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 3541 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 3601 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3661 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3721 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3781 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 3841 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 3901 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 3961 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 4021 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 4081 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 4141 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 4201 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 4261 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 4321 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 4381 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 4441 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 4501 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 4561 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 4621 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4681 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 4741 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 4801 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 4861 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 4921 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4981 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5041 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5101 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5161 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5221 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5281 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5341 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5401 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5461 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5521 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 5581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 5641 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 5701 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 5761 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 5821 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 5881 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 5941 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 6001 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 6061 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 6121 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 6181 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 6241 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 6301 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6361 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6421 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6481 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 6541 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6601 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 6661 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6781 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 6841 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6901 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6961 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7021 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7081 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7141 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7921 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7981 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8041 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8101 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 8161 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8221 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8281 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8341 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 8401 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8461 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8521 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8581 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8641 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8701 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8761 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8821 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8881 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8941 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 9001 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 9061 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 9121 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 9181 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 9241 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 9301 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 9361 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 9421 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.