pLV2-EF1a-3×FLAG-MYRFL-IRES-Pu

pLV2-EF1a-3×FLAG-MYRFL-IRES-Puro plasmid

PVT96864

pLV2-EF1a-3×FLAG-MYRFL-IRES-Puro plasmid Information

Plasmid name: pLV2-EF1a-3×FLAG-MYRFL(human)-IRES-Puro
Alias: NM_182530.3;bcm1377; C12orf15; C12orf28
Gene lenght: 2730bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-3×FLAG-MYRFL-IRES-Puro plasmid Description

pLV2-EF1a-3×FLAG-MYRFL-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1a-3×FLAG-MYRFL-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaATTAGCTT GGTACgaatt 3541 cgccaccatg gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa 3601 ggatgacgat gacaagggtg gaggcggatc aatggatgtg gtaggcgaaa atgaggccct 3661 gcagcagttc tttgaagctc agggagccaa tggtactctg gagaacccag ccctggacac 3721 aagtctgttg gaggaatttc tgggcaatga ctttgatttg ggggccttgc aacgccagct 3781 cccagacacc ccgccctatt ctgcatccga ctcatgctct cctccgcagg tcaaaggtgc 3841 atgctaccca accctgaggc ccacagctgg gaggactcca gctccttttc tccaccccac 3901 agctgctcct gcgatgccgc ccatgcaccc gctgcagagc acctctggaa tgggcgactc 3961 ctgccaaatc cacggaggct ttcacagctg ccactcaaac gccagtcatc ttgccacccc 4021 cctggaccaa tccgtgtcct cccatctggg gataggttgt tcttaccctc agcagcctct 4081 gtgtcacagc cctggagcct cattgcctcc aaccaagaag agaaagtgca cgcaggcact 4141 ggaggactcc ggggaatgcc gagtgtgggc ctgccactgc agaccgatga caagtaggag 4201 tcgcagcagt gaagtccagg accctgacag tgagggacag aacagaatgc ctacagacca 4261 gtgctctcct gctctgaagt ggcaaccatg ccatagtgtt ccttggcaca gcttattaaa 4321 cagtcattat gaaaaactac ctgatgtggg ctatcgagtt gtaacagaca aaggatttaa 4381 tttttcacca gcagatgaag cttttgtttg ccaaaagaag aatcatttcc agataaccat 4441 tcacatccaa gtttggggaa gtccaaaatt tgttgaaacc gagatgggcc taaagccaat 4501 agaaatgttt tacttgaaag tttttggcac taaggtggaa gctaccaatc aaataattgc 4561 tattgaacag tcccaagcag ataggagcaa aaagattttc aatcctgtta aaattgacct 4621 actggctgac caggtcacca aagtaacact gggacgatta cacttcagcg aaaccacagc 4681 aaataatatg agaaaaaagg gaaaaccaaa tccagaccag agatacttca tgttggtggt 4741 tggactgtat gctgctaacc aagaccagtt ctatctgttg tctgcccaca tctctgaaag 4801 gatcattgta agggcctcta accctgggca gtttgaaaat gacagtgatg cattgtggca 4861 gcgaggacaa gttccagaat ctattgtctg tcacggtcga gtaggaatca acacagatgc 4921 gccggacgaa gccctggttg tctgtggcaa catgaaagtg atggggacca tcatgcatcc 4981 ctctgacagc cgggcaaagc agaatatcca ggaggttgac acgaatgaac agctgaaaag 5041 aatagcccaa atgagaattg ttgaatatga ctacaaacct gaatttgcat ctgcaatggg 5101 aataaacact gcccatcaaa caggaatgat tgcccaggag gtgcaagaaa tcctgcccag 5161 agcagtaaga gaggttggtg atgtcacctg cggaaacgga gagaccttgg agaacttcct 5221 catggtggat aaggaccaga tctttatgga aaatgtaggt gcagtgaagc aactgtgcaa 5281 actaactaac aaccttgagg aaagaataga agagttagaa atatggaaca gaaagctggc 5341 ccggctaaag cggctcagta gttggaagtc atcagccagt gaagcaagca caatcagcaa 5401 atctagcaga gccgttagtg catcttctcc aagaagggcc gttcataaaa aaaacaacaa 5461 ggtttatttt tcaggaaaaa gacaggcgtg tcctaattgg gttttccaga ccttggttat 5521 aactctgatt gctgtgatgg cattttgtgc tttgacgata gttgccctct atatacttag 5581 cttaaaagat caagaccgac gtgtcccaaa tctccctcca agcaatatca caagctcaca 5641 ggagccagct ctgctgccca cagcctcctc ctcagcacct aatacatccc tggtaaccac 5701 accggcctcc ttacaagtac ctgaaattac tttctgtgaa atcctgccgt gtcaggagac 5761 ttattgctgc cccatccggg gaatgaaaga agtctcttca agtcctgtgc aaagacaatc 5821 tgaggagaag gaattccatc agaggcgatg gtcagaagac aaaagcaaat cagttttggc 5881 aagaaatgca ctcagcggcc ctgactggga gagtgactgg attgatacaa ccatcagttc 5941 tattcagatt atggaaatcc agcaaataat agatcatcag tattgcattc aaagcctcca 6001 gtgcggatct ggaaattata attacaatat tcctgttaat aaacacacac ccaccaatgt 6061 caagttctct ctggaaataa acacaacaga gccattgata gtcttccagt gcaaattcac 6121 ccttggaaat atatgtttcc atagtaaaag gggaaccaaa gggctggaaa gccacagaga 6181 aatctcccag gagatgacac agggatatca gcacatttgg agcctccctg tagccccatt 6241 ttctgacagc atgttccatt tccgtgtagc tgcaccggat ttagctgact gttcaactga 6301 tccttatttt gctgggatat tcttcaccga ttacttcttt tacttctatc gacgctgtgc 6361 ctaaactagt tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt 6421 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc 6481 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc 6541 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag 6601 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg 6661 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat 6721 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga 6781 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc 6841 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg 6901 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga 6961 tgataagctt gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg 7021 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg 7081 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg 7141 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 7201 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 7261 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 7321 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 7381 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 7441 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 7501 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 7561 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct 7621 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 7681 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 7741 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 7801 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 7861 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 7921 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 7981 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 8041 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 8101 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 8161 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc 8221 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 8281 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 8341 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga 8401 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 8461 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat 8521 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag 8581 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg 8641 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg 8701 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac 8761 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc 8821 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag 8881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 8941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 9001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 9061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat 9121 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 9181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 9961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 10021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 10981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 11341 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 11401 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 11461 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac 11521 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc 11581 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt 11641 agt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.