pLV3-CMV-3×FLAG-ALG8-EF1a-CopG

pLV3-CMV-3×FLAG-ALG8-EF1a-CopGFP plasmid

PVT96876

pLV3-CMV-3×FLAG-ALG8-EF1a-CopGFP plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-ALG8(human)-EF1a-CopGFP
Alias: NM_024079.5;CDG1H; PCLD3
Gene lenght: 1578bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-ALG8-EF1a-CopGFP plasmid Description

pLV3-CMV-3×FLAG-ALG8-EF1a-CopGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-ALG8-EF1a-CopGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagcgc 2281 CACCATGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAAGA 2341 CGATGACGAC AAGCGCGGGT CTAGAatggc ggcgctcaca attgccacgg gtactggcaa 2401 ttggttttcg gctttggcgc tcggggtgac tcttctcaaa tgccttctca tccccacata 2461 ccattccaca gattttgaag tacaccgaaa ctggcttgct atcactcaca gtttgccaat 2521 atcacagtgg tattatgagg caacttcaga gtggacgttg gattaccccc ctttctttgc 2581 atggtttgag tatatcctgt cacatgttgc caaatatttt gatcaagaaa tgctgaatgt 2641 ccataatttg aattactcca gctcaaggac cttacttttc cagagatttt ccgtcatctt 2701 tatggatgta ctctttgtgt atgctgtccg tgagtgctgt aaatgcattg atggaaaaaa 2761 agtgggtaaa gaacttacag aaaagccaaa atttattctg tcggtattac ttctgtggaa 2821 cttcgggtta ttaattgtgg accatattca ttttcagtac aatggctttt tatttggatt 2881 aatgctactc tccattgcac gattatttca gaaaaggcat atggaaggag catttctctt 2941 tgctgttctc ctacatttca agcatatcta cctctatgta gcaccagctt atggtgtata 3001 tctgctgcga tcctactgtt tcactgcaaa taaaccagat gggtctattc gatggaagag 3061 tttcagcttt gttcgtgtta tttccctggg actggttgtt ttcttagttt ctgctctttc 3121 attgggtcct ttcctggcct tgaatcagct gcctcaagtc ttttcccgac tctttccttt 3181 caagaggggc ctctgtcatg catattgggc tccaaacttc tgggctttgt acaatgcttt 3241 ggacaaagtg ctgtctgtca tcggtttgaa attgaaattt cttgatccca acaatattcc 3301 caaggcctca atgacaagtg gtttggttca gcagttccaa cacacagtcc ttccctcagt 3361 gactcccttg gcaaccctca tctgcacact gattgccata ttgccctcta ttttctgtct 3421 ttggtttaaa ccccaagggc ccagaggctt tctccgatgt ctaactcttt gtgccttgag 3481 ctcctttatg tttgggtggc atgttcatga aaaagccata cttctagcaa ttctcccaat 3541 gagccttttg tctgtgggaa aagcaggaga cgcttcgatt tttctgattc tgaccacaac 3601 aggacattat tccctctttc ctctgctctt cactgcacca gaacttccca ttaaaatctt 3661 actcatgtta ctattcacca tatatagtat ttcgtcactg aagactttat tcagaaaaga 3721 aaaacctctt tttaattgga tggaaacttt ctacctgctt ggcctggggc ctctggaagt 3781 ctgctgtgaa tttgtattcc ctttcacctc ctggaaggtg aagtacccct tcatcccttt 3841 gttactaacc tcagtgtatt gtgcagtagg catcacatat gcttggttca aactgtatgt 3901 ttcagtattg attgactctg ctattggcaa gacaaagaaa caaTAggatc cgcggccgcC 3961 TAATTAATTA AGGCCGCGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg 4021 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta 4081 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc 4141 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa 4201 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc 4261 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc 4321 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc 4381 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg 4441 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag 4501 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct 4561 gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct 4621 ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac 4681 caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta 4741 ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg 4801 cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt 4861 cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt 4921 ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca 4981 cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg 5041 cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca 5101 ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca 5161 cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt 5221 cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc 5281 caccggatct cgctaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac 5341 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 5401 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 5461 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 5521 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 5581 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 5641 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 5701 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 5761 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 5821 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 5881 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 5941 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc 6001 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 6061 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 6121 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 6181 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 6241 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 6301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 6361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 6421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 6481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 6541 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt 6601 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6661 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6721 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6781 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6841 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6901 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8701 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8761 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 8821 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 8881 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 8941 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9001 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9061 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9121 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9181 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.