pLV3-CMV-Stat5a-3×Myc-CopGFP-P

pLV3-CMV-Stat5a-3×Myc-CopGFP-Puro plasmid

PVT96854

pLV3-CMV-Stat5a-3×Myc-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Stat5a(mouse)-3×Myc-CopGFP-Puro
Alias: NM_011488.3;AA959963; STAT5
Gene lenght: 2382bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Stat5a-3×Myc-CopGFP-Puro plasmid Description

pLV3-CMV-Stat5a-3×Myc-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Stat5a-3×Myc-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg cgggctggat tcaggcccag cagcttcagg gagatgccct 2341 gcgccagatg caagtgttgt atgggcagca tttccccatc gaggtccggc actacctggc 2401 ccagtggatc gagagccagc cgtgggatgc tattgacttg gataatcccc aggaccgagg 2461 tcaggccacc caactcctgg agggcctggt gcaggagctg cagaagaagg cggagcacca 2521 ggtgggggaa gatgggtttt tgctgaagat caagctgggg cactatgcca cacagctcca 2581 gaacacgtat gaccgctgtc ccatggagct ggttcgctgt atccgtcaca ttctgtacaa 2641 cgaacagagg ctggttcgcg aagccaacaa ttgcagctcc cctgctggtg tcctggttga 2701 cgccatgtcc cagaagcacc ttcagatcaa ccaaaggttt gaggagctgc gcctgatcac 2761 acaggacacg gagaacgagc tgaagaagct gcagcagacc caagagtact tcatcatcca 2821 gtaccaggag agcctgcgga tccaagctca gtttgcccag ctgggccagc tgaaccccca 2881 ggagcgcatg agcagggaga cggccctcca gcagaagcaa gtgtccctgg agacctggct 2941 gcagcgagag gcacagacac tgcagcagta ccgagtggag ctggctgaga agcaccagaa 3001 gaccctgcag ctgctgcgga agcagcagac catcatcctg gacgacgagc tgatccagtg 3061 gaagcggaga cagcagctgg ccgggaacgg gggtcccccc gagggcagcc tggacgtgct 3121 gcagtcctgg tgtgagaagc tggccgagat catctggcag aaccggcagc agatccgcag 3181 ggctgagcac ctgtgccagc agctgcccat cccaggcccc gtggaggaga tgctggctga 3241 ggtcaacgcc accatcacgg acatcatctc agctctggtc accagcacgt tcatcatcga 3301 gaagcagcct cctcaggtcc tgaagaccca gaccaagttt gcggccaccg tgcgcctgct 3361 ggtgggggga aagctgaatg tgcacatgaa ccccccgcag gtgaaggcga ccatcatcag 3421 cgagcagcag gccaagtccc tgctcaagaa tgagaacacc cgcaatgagt gcagcggcga 3481 gatcctgaac aactgttgcg tcatggagta ccaccaggcc actggcacgc tcagcgccca 3541 cttcagaaac atgtcactga aaagaatcaa gcgcgccgac aggcgtggtg cagagtcggt 3601 gacggaggag aagttcacag tcctgtttga gtctcagttc agcgttggca gcaacgagct 3661 ggtgttccag gtgaagaccc tgtccctccc tgtggtcgtt atcgtccatg gcagccagga 3721 ccacaatgct actgccaccg tgctgtggga caatgccttt gctgagccgg gcagggtgcc 3781 atttgctgtg cctgacaagg tgctgtggcc gcagctgtgt gaagcgctca acatgaaatt 3841 caaggctgaa gtacagagca accggggctt gaccaaagag aacctcgtgt tcctggcaca 3901 gaaactgttc aacatcagca gcaaccacct cgaggactac aacagcatgt ctgtgtcctg 3961 gtcccagttc aaccgggaga acttgcccgg ctggaactac accttctggc agtggttcga 4021 cggggtgatg gaggtgctga agaagcacca taagccccat tggaatgatg gggctatcct 4081 gggtttcgtg aacaagcaac aggcccacga cctgctcatc aacaagccgg acgggacctt 4141 cctgctgcgc ttcagtgact cggaaatcgg gggcatcacc attgcttgga agtttgactc 4201 tccggaccga aacctctgga atctgaagcc attcacgacg cgagatttct ccattcggtc 4261 cctggccgac cggctggggg acctgaacta ccttatctac gtgttcccag accgacccaa 4321 ggacgaggtc tttgccaagt attacactcc tgtacttgcg aaagcagttg acggatacgt 4381 gaagccacag atcaagcaag tggtccctga gttcgtcaat gcatccacag atgccggagc 4441 cagcgccacc tacatggacc aggctccttc cccagtcgtg tgccctcaac ctcactacaa 4501 catgtaccca cccaaccctg accctgtcct tgaccaagat ggcgagtttg acctggatga 4561 gagcatggat gttgccaggc acgtggaaga acttttacgc cggcccatgg acagtctcga 4621 cgcccgcctc tccccacctg ctggtctctt cacctccgct agaagctccc tgtccggatc 4681 cGAACAAAAA CTAATATCAG AGGAAGATTT GGAACAGAAA CTAATCAGTG AAGAAGATTT 4741 AGAGCAAAAG TTAATTTCCG AGGAGGACTT ATAgcggccg cGaaggatct gcgatcgctc 4801 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 4861 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 4921 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 4981 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 5041 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 5101 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 5161 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 5221 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 5281 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 5341 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac 5401 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg 5461 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag 5521 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc 5581 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga 5641 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga 5701 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat 5761 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag 5821 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca 5881 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc 5941 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca 6001 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt 6061 ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac 6121 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct 6181 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 6241 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 6301 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 6361 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 6421 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 6481 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 6541 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 6601 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 6661 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 6721 ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga 6781 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 6841 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 6901 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 6961 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 7021 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 7081 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 7141 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 7201 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 7261 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc 7321 cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 7381 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa 7441 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 7501 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7561 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7621 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 7681 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 7741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 7801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 7861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7921 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 7981 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg 8041 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 8101 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 8161 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 8221 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 8281 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 8341 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 8401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 8461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 8521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 8581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 8641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 8701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 8761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 8821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 8881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 8941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 9001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 9061 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 9121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 9181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 9241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 9301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 9361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 9421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 9481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 9541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 9601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 9661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 9721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 9781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 9841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 9901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 9961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 10021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 10081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 10141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 10201 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt 10261 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 10321 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 10381 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 10441 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca 10501 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 10561 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 10621 tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.