pLV3-CMV-Amfr-3×Myc-CopGFP-Pur

pLV3-CMV-Amfr-3×Myc-CopGFP-Puro plasmid

PVT95671

pLV3-CMV-Amfr-3×Myc-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Amfr(mouse)-3×Myc-CopGFP-Puro
Alias: NM_011787.3;gp78
Gene lenght: 1917bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Amfr-3×Myc-CopGFP-Puro plasmid Description

pLV3-CMV-Amfr-3×Myc-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Amfr-3×Myc-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgc cgctgctctt cctcgagcgc ttcccgtggc ccagcctccg 2341 cacctacacg ggcctcagcg gcctggcctt gctgggcacc atcgtcagcg cctaccgcgc 2401 cctcagtcag ccggaggacg gctcgggtga gccggagccg ctaacggccc cactgcagcc 2461 cgaggcgcgc ctgaccgccg gcggcccccg ggcccgcgac gtggcccagt acctgctgtc 2521 ggacagcctg ttcgtgtggg ttctggtgaa tactgcttgc tgtgttttga tgttggtggc 2581 taagcttatc cagtgtattg tgttcggccc tcttcgagtg agtgaaagac agcatctcaa 2641 agacaagttt tggaatttta ttttctacaa gttcattttc atttttggag tgctgaatgt 2701 ccagactgtg gaggaagtgg tgatgtggtg cctctggttt gctggacttg tcttcctgca 2761 tctgatggtt cagctctgca aagacagatt tgaatacctt tccttttctc ccaccactcc 2821 aatgagcagc catgggagag tcctgtccct gctgattgct atgctgctct cctgctgtgg 2881 actggcagtc gtctgctgtg tcacaggcta cacacatggc atgcacacct tggctttcat 2941 ggctgcagag tctcttcttg tgaccgtgag aacagctcat gtgattttaa gatatgtaat 3001 tcacctctgg gacctcaacc acgaagggac gtgggaagga aagggaacct atgtgtatta 3061 caccgacttt gtcatggagc tcgctctcct gtccctggac ctcatgcacc acatccacat 3121 gctgttattt ggcaacatct ggttatctat ggctagcttg gtcatcttta tgcaacttcg 3181 ttacctgttc catgaggtac agcgtcgaat ccgtcgacac aagaattatt tgcgagtggt 3241 aggaaacatg gaagccaggt ttgctgttgc aactccagag gaactagctg tcaataatga 3301 tgactgtgca atctgctggg actccatgca ggctgcaagg aaactgccct gtggacatct 3361 ttttcacaac tcctgccttc gttcttggct agaacaagac acctcttgtc caacatgcag 3421 aatgtctctt aatattgctg atggcagtcg tgccagggaa gaccatcaag gagagaacct 3481 ggacgagaac ttggttcctg tagcagctgc cgaaggcagg cctcgcttga accagcacaa 3541 tcacttcttc cacttcgatg ggtcccggat tgcaagctgg ctgccaagtt tttcagttga 3601 agtgatgcac actaccaaca ttcttggcat cacgcaggca agcaactctc agctaaatgc 3661 catggctcat cagattcagg agatgttccc tcaggtcccg taccaccttg tgctccagga 3721 cctccagatg acacgctccg tggagataac aacagataac atcttagaag gacggattca 3781 agtacctttc cccacgcagc ggtcggatag cctgaggcct gcactgaaca gcccagtgga 3841 gaggcccagc cctgacctgg aggagggaga agcttctgtc cagacggagc gtgtaccact 3901 ggacctcagt cctcgcctag aggagacctt ggacttcagt gaggtagaac tagagcctat 3961 tgaggtggaa gactttgagg cccgtggaag ccgcttctcc aagtctgctg atgagagaca 4021 gcgcatgtta gttcagcgca aggatgacct cctgcagcag gctcgaaagc ggttcttaaa 4081 caaaagttct gaagatgatg gggcctcaga gagactcctc ccttctgaag gcacatcctc 4141 tgaccccgtg accctgcgcc gaaggatgct ggcagccgct gctgagcgga gacttcagag 4201 gcaacggaca accggatccG AACAAAAACT AATATCAGAG GAAGATTTGG AACAGAAACT 4261 AATCAGTGAA GAAGATTTAG AGCAAAAGTT AATTTCCGAG GAGGACTTAT AgcggccgcG 4321 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc 4381 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt 4441 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc 4501 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac 4561 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg 4621 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac 4681 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct 4741 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca 4801 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc 4861 gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga 4921 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg 4981 cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt 5041 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc 5101 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg 5161 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc 5221 cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat 5281 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga 5341 taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag 5401 cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa 5461 cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg 5521 catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca 5581 gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg 5641 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta 5701 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 5761 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 5821 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 5881 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 5941 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 6001 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 6061 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 6121 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 6181 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 6241 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct 6301 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 6361 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6421 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6481 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6541 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 6601 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 6661 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 6721 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 6781 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc 6841 tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac 6901 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 6961 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga 7021 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 7081 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 7141 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 7201 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 7261 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 7321 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 7381 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7441 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7501 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg 7561 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 7621 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 7681 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 7741 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 7801 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 7861 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 7921 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 7981 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 8041 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 8101 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 8161 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 8221 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 8281 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 8341 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 8401 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 8461 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 8521 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 8581 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 8641 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 8701 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 8761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 8821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 8881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 8941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 9001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 9061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 9121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 9181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 9241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 9301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 9361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 9421 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 9481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 9541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 9601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 9661 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 9721 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 9781 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 9841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 9901 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 9961 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 10021 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 10081 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 10141 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.